有氧运动对高糖高脂膳食大鼠腓肠肌Nrf2-SOD的影响*

doi:10.12047/j.cjap.6004.2020.102

中国应用生理学杂志 ›› 2020, Vol. 36 ›› Issue (5): 481-485.doi: 10.12047/j.cjap.6004.2020.102

有氧运动对高糖高脂膳食大鼠腓肠肌Nrf2-SOD的影响^*

刘玉倩^1,2, 张静³, 高丽娜³, 王海涛^1,2△

1.岭南师范学院体育科学学院,
2.运动与健康研究所, 广东湛江 524048;
3.河北师范大学体育学院, 河北石家庄 050024

收稿日期:2020-01-08 修回日期:2020-07-09 发布日期:2021-02-25
通讯作者: ^△Tel: 17875191037; E-mail: haitaoyq@126.com
基金资助:
^*河北省高等学校自然科学重点项目(ZD2017048);岭南师范学院人才专项(ZL2008,ZL2009)

Effects of aerobic exercise on Nrf2-SOD pathway in the gastrocnemius of rats with high-glucose and high-fat diet

LIU Yu-qian^1,2, ZHANG Jing³, GAO Li-na³, WANG Hai-tao^1,2△

1. School of Sport and Exercise Science, Lingnan Normal University, Zhanjiang 524048;
2. In stitute of Exercise and Health, Lingnan Normal University, Zhanjiang 524048;
3. Department of Physical Education, Hebei Normal University, Shijiazhuang 050024, China

Received:2020-01-08 Revised:2020-07-09 Published:2021-02-25

摘要/Abstract

摘要： 目的: 探讨有氧运动预防大鼠胰岛素抵抗中Nrf2及SOD的变化。方法: 24只12月龄SD大鼠随机分为对照组(C)、高糖高脂IR组(IR)和高糖高脂IR并运动组(IRE)。IRE进行递增负荷跑台运动,运动6周。检测腓肠肌T-SOD、CAT、MDA、GSH/GSSG,ELISA法检测腓肠肌8-OHdG含量,Western blot检测腓肠肌Nrf2和GLUT4表达。结果: ①IRE组HOMA-IR明显低于IR组(P＜0.05);IRE组肌糖原明显高于IR组(P＜0.01);②IRE组T-SOD和CAT、GSH/GSSG明显高于IR组(P＜0.01);IRE组8-OHdG和MDA明显低于IR组(P＜0.01);③IRE组腓肠肌Nrf2和GLUT4明显高于IR组 (P＜0.01) 。结论: 有氧运动可激活大鼠腓肠肌Nrf2-SOD通路,提高抗氧化酶活性和糖摄取能力,预防IR发生。

Abstract: Objective: To investigate the changes of nuclear factor erythroid 2-related factor 2 (Nrf2) and superoxide dismutase (SOD) in the prevention of insulin resistance in skeletal muscle of rats by aerobic exercise. Methods: Twenty-four 12-month old Sprague-Dawley (SD) rats were randomly divided into control (C), insulin resistance model (IR) and insulin resistance model and exercise (IRE) groups. IR was induced by high- glucose and high-fat diet. Rats of IRE received the increasing load running on the treadmill for 6 W. The activity of total superoxide dismutase (T-SOD), catalase (CAT), Malonaldehyde (MDA) and glutathione / L-glutathione oxidized (GSH/GSSG) were measured by experiment kits. The content of 8-hydroxy-2 deoxyguanosine (8-OHdG) was measured by ELISA Kits. The expressions of Nrf2 and Glucose transporters 4 (GLUT4) in gastrocnemius were detected by Western Blot. Results: ①Compared with IR, the content of HOMA-IR was decreased in IRE (P＜0.05), while the content of muscle glycogen was increased in IRE (P＜0.01). ② The activities of T-SOD, CAT, and GSH/GSSG in IRE were significantly increased compared with those in IR (P＜0.01). Compared with IR, the contents of 8-OHdG and MDA in IRE were significantly decreased (P＜0.01). ③ The expressions of Nrf2 and GLUT4 in gastrocnemius of IRE rats were increased than those in IR (P＜0.01). Conclusion: The Nrf2 pathway was activated in gastrocnemius of rats after aerobic exercise, which promoted the activity of antioxidant enzymes, and prevented IR induced by high- glucose and high-fat diet.

Key words: Nrf2, aerobic exercise, gastrocnemius, insulin resistance, rat

中图分类号:

G804.5

刘玉倩, 张静, 高丽娜, 王海涛. 有氧运动对高糖高脂膳食大鼠腓肠肌Nrf2-SOD的影响^*[J]. 中国应用生理学杂志, 2020, 36(5): 481-485.

LIU Yu-qian, ZHANG Jing, GAO Li-na, WANG Hai-tao. Effects of aerobic exercise on Nrf2-SOD pathway in the gastrocnemius of rats with high-glucose and high-fat diet[J]. CJAP, 2020, 36(5): 481-485.

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有氧运动对高糖高脂膳食大鼠腓肠肌Nrf2-SOD的影响^*

Effects of aerobic exercise on Nrf2-SOD pathway in the gastrocnemius of rats with high-glucose and high-fat diet

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