长期中小强度有氧运动对大鼠左心室肌蛋白质组差异表达的影响*

doi:10.12047/j.cjap.6081.2021.091

中国应用生理学杂志 ›› 2021, Vol. 37 ›› Issue (6): 673-677.doi: 10.12047/j.cjap.6081.2021.091

长期中小强度有氧运动对大鼠左心室肌蛋白质组差异表达的影响^*

郭远盘¹, 史绍蓉^2△, 徐哲³, 王娟⁴, 黄思敏⁵, 万莉莉⁶, 雷雄⁶, 彭勇⁷

1.长沙师范学院体育科学学院, 湖南长沙 410010;
2.湖南师范大学体育学院, 湖南长沙 410012;
3.湖南城市学院体育学院, 益阳 413039;
4.北京体育大学, 海淀 100084;
5.肇庆学院体育与健康学院, 广东肇庆 526061;
6.中南大学湘雅医院, 湖南长沙 410008;
7.南京体育学院运动健康科学系, 江苏南京 210014

收稿日期:2020-04-24 修回日期:2021-03-01 出版日期:2021-11-28 发布日期:2021-11-25
通讯作者: ^△Tel: 13975167236; E-mail: Shisrfl@126.com
基金资助:
^*湖南省科技厅计划项目(2007FJ3075);湖南省教育厅科学研究项目(19C0145)

Effects of long-term moderate-small intensity aerobic exercise on differential expression of proteome in left ventricular muscle of rats

GUO Yuan-pan¹, SHI Shao-rong^2△, XU Zhe³, WANG Juan⁴, HUANG Si-min⁵, WAN Li-li⁶, LEI Xiong⁶, PENG Yong⁷

1. P. E. Dept.,Changsha Normal University, Changsha 410010;
2. P. E. Dept., Hunan Normal University, Changsha 410012;
3. P. E. Dept., Hunan City University, Yiyang 413039;
4. Beijing Sport University, Beijing 100084;
5. Sports and Health Department, Zhaoqing University, Zhaoqing 526061;
6. Xiangya Hospital, Central South University, Changsha 410008;
7. Nanjing Sport Institute, Nanjing 210014, China

Received:2020-04-24 Revised:2021-03-01 Online:2021-11-28 Published:2021-11-25

摘要/Abstract

摘要： 目的:探讨长期中小强度有氧运动对大鼠左室肌蛋白质组差异性表达的影响,筛选出对中小强度有氧运动刺激敏感的目标蛋白质,丰富运动健身的基础理论及为慢性心血管疾病的康复治疗提供新的思路和实验依据。方法:20只雄性SD大鼠随机分为运动组(E组)和对照组(C组)(n=10)。建立大鼠长期中小强度有氧运动跑台训练模型,采用双向凝胶电泳(2-DE)对大鼠左心室肌全蛋白样品进行分离。采用串联飞行时间质谱蛋白质仪对部分分离后差异表达量上调大于5倍或下调超过80%的蛋白质点进行质谱鉴定。结果:与C组比较,E组大鼠心脏重量指数(HWI)增加了32.0%,具有显著性差异(P＜0.05);与C组比较,E组有71个蛋白质点表达上调≥2倍或下调≥50%,对4个表达上调≥5倍或下调大于等于80%的蛋白质点进行质谱鉴定,鉴定出3个蛋白质和1个未知蛋白质。结论:长期中小强度有氧运动后,大鼠心脏发生了良好的适应性改变;大鼠左心室肌蛋白质组发生了明显变化,长期中小强度有氧运动能有效增强大鼠心肌抗氧化能力。

关键词: 蛋白质组, 左心室肌, 中小强度有氧运动, 双向凝胶电泳, 质谱鉴定

Abstract: Objective: To investigate the effects of long-term moderate-small intensity aerobic exercise on the differential expression of proteome in left ventricular muscle of rats, and to screen the target proteins sensitive to moderate-small intensity aerobic exercise stimulation. This study will enrich the basic theory of exercise and fitness and provide new ideas and experimental basis for the rehabilitation treatment of chronic cardiovascular disease. Methods: Twenty male SD rats were randomly divided into exercise group and control group (n=10). The treadmill training model of long-term moderate-small intensity aerobic exercise was established, and the whole protein samples of left ventricular muscle were extracted and separated by two-dimensional gel electrophoresis (2-DE). The two-dimensional gel electrophoresis map was analyzed by Bio PD quest image analysis software. The protein spots with differential expression more than 5 times or down-regulated over 80% after exercise were identified by tandem time-of-flight mass spectrometry (ULGRAFL-FLEX-TOF/TOF). Results: Compared with the group C, the heart weight index of the group E was increased by 32.0%, and the difference was significant (P＜0.05). Compared with the group C, there were 71 protein spots expression were up-regulated≥2 times or down-regulated≥50% in the group E. 4 protein spots expression were up-regulated≥5 times or down-regulated≥80% were identified by mass spectrometry, 3 proteins and 1 unknown protein were identified. Conclusion: After long-term moderate-small intensity aerobic exercise, the rats heart had a good adaptive change, and the proteome of left ventricular muscle changed significantly. Long-term moderate-small intensity aerobic exercise can effective enhance the ability of myocardial antioxidation.

Key words: proteome, left-ventricular muscle, moderate-small intensity aerobic exercise, two dimensional gel electrophoresis, mass spectrometry identification

中图分类号:

G804

郭远盘, 史绍蓉, 徐哲, 王娟, 黄思敏, 万莉莉, 雷雄, 彭勇. 长期中小强度有氧运动对大鼠左心室肌蛋白质组差异表达的影响^*[J]. 中国应用生理学杂志, 2021, 37(6): 673-677.

GUO Yuan-pan, SHI Shao-rong, XU Zhe, WANG Juan, HUANG Si-min, WAN Li-li, LEI Xiong, PENG Yong. Effects of long-term moderate-small intensity aerobic exercise on differential expression of proteome in left ventricular muscle of rats[J]. CJAP, 2021, 37(6): 673-677.

参考文献

[1] Guo H, Isserlin R, Emili A, et al. Exercise-responsive phosphoproteins in the heart[J]. J Mol Cell Cardiol, 2017, 111: 61-68.
[2] Jayasundara N, Tomanek L, Dowd WW, et al. Proteomic analysis of cardiac response to thermal acclimation in the eurythermal goby fish Gillichthys mirabilis[J]. J Exp Biol, 2015, 218: 1359-1372.
[3] Dorr KM, Conlon FL. Proteomic-based approaches to cardiac development and disease[J]. Curr Opin Chem Biol, 2019, 48: 150-157.
[4] Wang X, Shen X, Weil BR, et al. Quantitative proteomic and phosphoproteomic profiling of ischemic myocardial stunning in swine[J]. Am J Physiol Heart C, 2020, 318(5): H1256-H1271.
[5] Rogers DS, Li ZB, Randall MJ, et al. Identification of candidate proteins for the cardioprotective effects of exercise[J]. Med Sci Sports Exerc, 2003, 35(5): 367-371.
[6] McMullen JR, Jennings GL. Differences between pathological and physiological cardiac hypertrophy: novel therapeutic strategies to treat heart failure[J]. Clin Exp Pharmacol Physiol, 2007, 34(4): 255-262.
[7] 史绍蓉, 刘田, 龚丽, 等. 一次性力竭运动对大鼠心房肌蛋白质组表达的影响[J]. 中国运动医学杂志, 2009, 28(4): 391-394.
[8] 刘建荣. 运动心脏重塑过程中大鼠心房肌的比较蛋白质组学研究 [D]. 湖南师范大学, 2008.
[9] 徐哲, 史绍蓉, 谭军, 等. 中小强度有氧运动后大鼠右心室肌蛋白质组差异表达的研究[J]. 北京体育大学学报, 2010, 33(10): 48-51.
[10] 龚丽, 史绍蓉, 黄鹰, 等. 递增负荷运动对大鼠心房肌蛋白质组研究的影响[J]. 中国康复医学杂, 2008(2): 107-109.
[11] 史绍蓉, 袁爱国, 王娟, 等. 中等强度运动对大鼠心室肌蛋白质组差异表达的影响[J]. 体育科学, 2008, 28(12): 56-61.
[12] 王娟. 中等强度有氧运动大鼠右心室肌蛋白质组的差异性表达 [D]. 湖南师范大学, 2009.
[13] 万莉莉, 史绍蓉, 王娟, 等. 4周不同强度运动对大鼠右心室肌蛋白质组差异表达的影响[J]. 北京体育大学学报, 2012, 35(1): 55-58.
[14] Hsieh YY, Chang CC, Hsu KH, et al. Effect of exercise training on calpain systems in lean and obese Zucker rats [J]. Int J Biol Sci, 2008, 4(5): 300-308.
[15] 袁爱国. 运动心脏重塑过程中大鼠左室肌蛋白质组差异表达的研究[D]. 湖南师范大学, 2008.
[16] Grg A, Obermaier C, Boguth G, et al. The current state of two-dimensional electrophoresis with immobilized pH gradients[J]. Electrophoresis, 2000, 21(6): 1037-1053.
[17] 刘田, 史绍蓉, 龚丽, 等. 长期递增负荷训练后大鼠心室肌蛋白质组的差异性表达[J]. 中国运动医学杂志, 2008, 27(6): 697-701.
[18] 王世强, 常芸, 饶志坚, 等. 运动性心脏重塑:microRNA的调节[J]. 体育科学, 2017, 37(11): 81-90.
[19] 彭勇, 史绍蓉, 黄思敏, 等. 4周中等强度有氧运动诱导大鼠心房肌蛋白质组差异表达的研究[J]. 中国应用生理学杂志, 2018, 34(5): 450-456.
[20] Nomura M, Kato K, Nagasaka A, et al. Serum beta-enolase in acute myocardial infarction [J]. Br Heart J, 1987, 58(1): 29-33.
[21] Alkireidmi MA, Al-Abbasi FA, Mehanna MG, et al. Biochemical markers as diagnostic/prognostic indicators for ischemic disease [J]. Afr Health Sci, 2018, 18(2): 287-294.
[22] 郄涛, 徐鹏, 张丙信, 等. 红景天苷对力竭大鼠心肌细胞凋亡通路的影响[J]. 中国应用生理学杂志, 2019, 35(4): 376-380.
[23] 周宝宏. 鸟苷酸结合蛋白信号转导的分子机制[J]. 生物化学与生物物理进展, 1989, 16(5): 332-337.
[24] 唐杰, 曹传平, 简一平, 等. cAMP依赖的蛋白激酶PKAR_(Iα)亚基在胃癌细胞中的表达及8-溴-环核苷酸对其表达及细胞增殖的影响[J]. 空军总医院学报, 2001, 17(4): 203-205.
[25] Sandrini F, Matyakhina L, Sarlis NJ, et al. Regulatory subunit type I-alpha of protein kinase A (PRKAR1A): a tumor-suppressor gene for sporadic thyroid cancer [J]. Gene Chromosome Canc, 2002, 35(2): 182-192.

长期中小强度有氧运动对大鼠左心室肌蛋白质组差异表达的影响^*

Effects of long-term moderate-small intensity aerobic exercise on differential expression of proteome in left ventricular muscle of rats

PDF (PC)

可视化

摘要/Abstract

引用本文

使用本文

参考文献

相关文章 1

编辑推荐

Metrics

本文评价