有氧运动联合螺旋藻多糖对糖尿病大鼠学习记忆能力的影响及其机制*

doi:10.12047/j.cjap.6065.2021.058

摘要/Abstract

摘要： 目的:研究有氧运动联合螺旋藻多糖对糖尿病大鼠学习记忆能力及对海马脑组织p75^NTR信号相关蛋白的影响。方法:采用高糖高脂饮食喂养4周配合低剂量腹腔注射的方法复制Ⅱ型糖尿病大鼠实验模型。成模后随机分为:模型组(B组)、运动+糖尿病组(C组)、螺旋藻多糖+糖尿病组(D组)、运动+螺旋藻多糖+糖尿病组(E组),另设正常对照组(A组)。共5组,每组12只。C组和E组施加6周的有氧游泳训练,D组和E组给予螺旋藻多糖灌胃6周,A组不施加任何干预。用Morris水迷宫检测大鼠学习记忆能力;Tunel染色法检测神经元细胞凋亡情况;ELISA法检测BDNF含量,Western blot法检测p75^NTR和cleaved caspase-3蛋白表达,免疫组化法检测cleaved caspase-3表达变化。同时观察大鼠随机血糖、血胰岛素等指标的变化。结果:①与A组比较,B组不同时间点上的体重均显著降低(P＜0.01);与B组比较,C、D、E组在不同时间点上的体重差异均无统计学意义(P均＞0.05)。与A组比较,B组的血糖和胰岛素水平显著升高(P＜0.01);与B组比较,干预各组的血糖和胰岛素水平显著降低(P＜0.05或P＜0.01)。②与A组比较,B组寻找平台的逃避潜伏期明显延长(P＜0.01),目标象限时间和穿越平台次数显著减少(P＜0.01);与B组比较,各干预组逃避潜伏期均显著缩短(P＜0.05或P＜0.01),穿越平台次数均显著增加(P＜0.05或P＜0.01),其中以E组效果最好。③与B组比较,干预各组海马神经细胞凋亡减少,p75^NTR、cleaved caspase-3蛋白表达显著降低(P＜0.05或P＜0.01),BDNF含量明显增加(P＜0.05或P＜0.01)。其中以E组效果更为明显。结论:有氧运动与螺旋藻多糖能有效改善糖尿病大鼠学习记忆,其中以两者联合组的效果更为显著,其机制可能与其更好的调节p75^NTR信号相关蛋白的表达,一定程度抑制细胞凋亡,从而有效改善Ⅱ型糖尿病学习记忆,发挥神经保护作用有关。

关键词: 有氧运动, 螺旋藻多糖, p75^NTR信号, 细胞凋亡, 学习记忆

Abstract: Objective: To investigate the effects of aerobic exercise plus spirulina polysaccharide(SP) supplement on the related protein expressions of p75^NTRsignal in hippocampal and the improvement of learning and memory of type 2 diabetes rats. Methods: The model of type 2 diabetic rats was established by fed high-fat diet for four weeks together with intraperitoneal injecting a low dose of STZ. The model rats were randomly divided into diabetic model group(B),diabetic exercise group(C),diabetic+SP group(D)and diabetic exercise+SP group(E), another normal control group(A)without any intervention was set up,12 rats in each group. The rats in Group C and E were treated with intervention of swimming training for six weeks. The rats in Group D and E were treated with SP intragastrically for 6 weeks. Learning and memory abilities were observed by Morris water maze test. The hippocampus cell apoptosis was observed by Tunnel staining, and BDNF content and the expressions of p75^NTR, cleaved caspase-3 of hippocampus were tested by ELISA, Western blot and immunohistochemistry, respectively. At the same time, the changes of blood glucose and levels of serum insulin were examined. Results: ①Compared with Group A at different time points, the body weight of Group B was decreased significantly(P＜0.01). Compared with Group B at different time points, the body weight of Group C,D and E had no difference (P＞0.05). Compared with Group A, levels of the blood glucose and serum insulin Group B were increased significantly(P＜0.01).Compared with Group B, the levels in the intervention groups were decreased significantly (P＜0.01); ②Compared with Group A, the escape latencies of Group B were prolonged significantly(P＜0.01), platform quadrant residence duration and the times of crossing platform were decreased (P＜0.01). Compared with Group B, the escape latencies of the intervention groups were shortened (P＜0.05 or P＜0.01), and the times of crossing platform were increased (P＜0.05 or P＜0.01). ③Compared with Group B, the neural cells apoptosis of the intervention rats was decreased, and the protein expressions of p75^NTR and cleaved caspase-3 were decreased (P＜0.05 or P＜0.01), however the expression of BDNF was increased significantly (P＜0.05 or P＜0.01). Conclusion: Aerobic exercise and SP supplement can improve the learning-memory ability of type 2 diabetes rats, and the improvement effect of exercise combined with SP is markedly better than that of exercise and SP alone, the mechanism might be related to better regulating p75^NTR signal related protein expressions and then inhibiting apoptosis in hippocampus of rats with type 2 diabetes.

Key words: aerobic exercise, spirulina polysaccharide, p75^NTR signal, cell apoptosis, learning and memory

中图分类号:

G804
R49

朱洪竹, 朱梅菊, 张莹. 有氧运动联合螺旋藻多糖对糖尿病大鼠学习记忆能力的影响及其机制^*[J]. 中国应用生理学杂志, 2021, 37(6): 665-672.

ZHU Hong-zhu, ZHU Mei-ju, ZHANG Ying. The effects of aerobic exercise plus spirulina polysaccharide supplement on learning and memory function of diabetes rats and its mechanism[J]. CJAP, 2021, 37(6): 665-672.

参考文献

[1] McCrimmon RJ, Ryan CM, Frier BM. Diabetes and cognitive dysfunction[J]. Lancet, 2012, 379 (9833): 2291-2299.
[2] Domínguez RO, Pagano MA, Marschoff ER, et al. Alzheimer disease and cognitive impairment associated with diabetes mellitus type 2: associations and a hypothesi[J]. Neurol, 2014, 29(9): 567-572.
[3] 李和教, 周吉银, 刘远志. 2型糖尿病认知功能障碍的作用机制研究进展[J]. 生理科学进展, 2018, 49(2): 110-114.
[4] 林智君, 陈煜森, 钟望涛, 等. 螺旋藻多糖对阿尔茨海默病小鼠脑线粒体呼吸链酶活性的影响[J]. 中华脑科疾病与康复杂志, 2016, 6(2): 79-82.
[5] 常雪莹, 苏仪, 张汝南, 等. 螺旋藻多糖对糖尿病大鼠血糖及胰岛素水平的影响[J]. 职业与健康, 2016, 32 (17): 2348-2350.
[6] 朱洪竹, 朱梅菊, 曾志刚, 等. 有氧运动加螺旋藻补充对2型糖尿病大鼠肾脏TLR4/ NF-κB-p65 的表达及炎性因子的影响[J]. 天然产物研究与开发, 2017, 29(7): 1119-1126.
[7] 徐求波, 黄涛, 季浏. 跑台训练增强大鼠的学习记忆及其海马BDNF基因表达[J]. 北京体育大学学报, 2011, 34(4): 51-53.
[8] Longhi L, Perego C, Ortolano F, et al. Tumor necrosis factor in traumatic brain injury: Effects of genetic deletion of p55 or p75 receptor [J]. J Cereb Blood Flow Metab, 2013, 33(8): 1182-1189.
[9] Sltys Z, Janeczko K, Orzylowska-Sliwinska O, et al. Morphological transformations of cells immunopositive for GFAP, TrkA of p75 in the CA1 hippocampal area following transient global ischemia in the rat. A quantitative study[J]. Brain Res, 2003, 987(2): 186-193.
[10] 张惠莉. 骨髓间充质干细胞移植改善慢性脑缺血大鼠空间学习记忆能力和BDNF及P75NTR表达的影响[D]. 郑州大学, 2013.
[11] Reed MJ, Meszaros K, Entes LJ, et al. A new rat model of type 2 diabetes: the fat fed, streptozotoc in treated rat[J]. Metabolism, 2000, 49: 1390-1394.
[12] Ploug T, Stallknecht BM, Pedersen D, et al. Effeet of endurance training on glucose transport capacity and glucose transporter expression in rat skeletal muscle [J]. A m J Physiol, 1990, 259: 778-786.
[13] Liu YW, Zhu X, Yang QQ, et al. Suppression of methylglyoxal hyperactivity by mangiferin can prevent diabetes-associated cognitive decline in rats [J]. Psychopharmacology (Berl), 2013, 228 (4): 585-594.
[14] Li J, Liu Y, Liu B, et al. Mechanisms of aerobic exercise upregulating the expression of hippocampal synaptic plasticity-associated proteins in diabetic rats [J]. Neural Plast, 2019, 2019(3): 1-12.
[15] 张风河, 魏奉才, 杨丕山. p75NTR对小鼠面神经损伤后脑内神经元再生的影响[J]. 口腔颌面外科杂志, 2007, 17(2): 139-142.
[16] Wu LL, Cai WP, Lei X, et al. NRAL mediates cisplatin resistance in hepatocellular arcinoma via miR-340-5p/Nrf2 axis[J]. J Cell Communica Signaling, 2018, 12: 1-14.
[17] 杨德森, 干国平, 李浩浩, 红景天苷对阿尔茨海默病模型大鼠学习记忆能力和海马组织Aβ含量及p75NTR表达的影响[J]. 医药导报, 2017, 36(2): 141-144.
[18] 高长越, 方传勤, 许志强. 阻断内源性 P75NTR 对阿尔茨海默病小鼠海马神经干细胞增殖与学习记忆的影响[J]. 第三军医大学学报, 2010, 32(11): 1152-1154.
[19] 李翰, 王松涛, 常芸. 有氧运动和白藜芦醇对糖尿病大鼠海马神经元凋亡的影响[J]. 中国体育科技, 2018, 54(5): 77-87.
[20] 李艳荣. 有氧运动对慢性疲劳综合征建模大鼠海马 ERK/CREB/BDNF 信号通路的影响[J]. 山东体育学院学报, 2020, 36(2): 97-105.
[21] 朱洪竹, 张莹, 朱梅菊, 等. 螺旋藻对运动疲劳大鼠海马损伤的保护作用及机制研究[J]. 中国应用生理学杂志, 2018, 34(6): 562-567.
[22] 崔晶晶, 高俊虹, 王玉敏, 等. 针药结合增效机制研究的新思路探讨[J]. 针刺研究, 2010, 35 (2): 146-150.
[23] 崔笑梅, 曹建民, 张静, 等. 有氧运动联合黑果枸杞对高脂膳食大鼠心肌脂代谢某些指标的影响[J]. 中国应用生理学杂志, 2020, 36(4): 301-305.

有氧运动联合螺旋藻多糖对糖尿病大鼠学习记忆能力的影响及其机制^*

The effects of aerobic exercise plus spirulina polysaccharide supplement on learning and memory function of diabetes rats and its mechanism

PDF (PC)

可视化

摘要/Abstract

引用本文

使用本文

参考文献

相关文章 15

编辑推荐

Metrics

本文评价

[1]	郭远盘, 史绍蓉, 徐哲, 王娟, 黄思敏, 万莉莉, 雷雄, 彭勇. 长期中小强度有氧运动对大鼠左心室肌蛋白质组差异表达的影响^*[J]. 中国应用生理学杂志, 2021, 37(6): 673-677.
[2]	丁璐, 邵淑丽, 何孟奇, 黄鑫, 张伟伟, 张珍珠. 桦木酸对胃癌SGC-7901细胞凋亡的影响^*[J]. 中国应用生理学杂志, 2021, 37(5): 495-499.
[3]	樊小群, 李欢, 朱华雄, 黄健平, 何吕芬. Mir-335-5p靶向G6PD对结肠癌细胞增殖、凋亡的影响[J]. 中国应用生理学杂志, 2021, 37(4): 402-406.
[4]	王瑜, 何施燕, 朱若婷, 柯瑞君, 陈佳玉. 紫草素对肝癌细胞SMMC-7721凋亡的影响及其机制[J]. 中国应用生理学杂志, 2021, 37(4): 415-418.
[5]	樊华, 邵淑丽, 何孟奇, 黄鑫, 张伟伟, 张珍珠. 不同浓度桦木酸对人胃癌MGC-803细胞凋亡的影响[J]. 中国应用生理学杂志, 2021, 37(4): 419-422.
[6]	牛衍龙, 曹建民, 周绮云, 胡戈, 郭娴, 刘健, 郝敏, 张涛, 周海涛. 虾青素复合有氧运动对D-半乳糖诱导大鼠肾脏衰老的干预作用及其机制[J]. 中国应用生理学杂志, 2021, 37(4): 433-438.
[7]	徐兴军, 李珊珊, 刘畅, 张伟伟, 张珍珠, 邵淑丽. 川楝素诱导人胃癌MGC-803细胞凋亡及其机制[J]. 中国应用生理学杂志, 2021, 37(3): 262-265.
[8]	王友华, 陈伟, 马美, 戴高远, 滑艺杰. 不同强度有氧运动对大鼠房室结形态结构及VEGF、VIP、Syn表达的影响[J]. 中国应用生理学杂志, 2021, 37(3): 287-292.
[9]	江涛, 韦雪亮, 肖书奇. 谷氨酰胺对大鼠运动性疲劳的改善作用及其机制[J]. 中国应用生理学杂志, 2021, 37(3): 293-296.
[10]	安方玉, 颜春鲁, 刘永琦, 王彩霞, 苏韫, 石彪, 杨双红, 郭丹, 蒋国凤. 黄芪汤对延缓C离子辐射致大鼠肾组织细胞凋亡的影响及相关机制[J]. 中国应用生理学杂志, 2021, 37(3): 318-323.
[11]	王丹, 高峰, 陈慧, 项伟玲, 骆志显, 金丽琴. 人参归脾丸对脾不统血证大鼠肝损伤的保护作用及其机制^*[J]. 中国应用生理学杂志, 2020, 36(6): 571-575.
[12]	吕紫嫣, 张肖艳, 陈弘, 蒋盼若, 陈佳玉. SmacN7诱导乳腺癌细胞MDA-MB-157凋亡的作用及其机制^*[J]. 中国应用生理学杂志, 2020, 36(6): 642-647.
[13]	高扬, 于亮, 付悦, 王璐, 郭春杰. 4周电针干预对大鼠白色脂肪“棕色化”的影响及其机制^*[J]. 中国应用生理学杂志, 2020, 36(5): 471-477.
[14]	刘玉倩, 张静, 高丽娜, 王海涛. 有氧运动对高糖高脂膳食大鼠腓肠肌Nrf2-SOD的影响^*[J]. 中国应用生理学杂志, 2020, 36(5): 481-485.
[15]	崔笑梅, 曹建民, 张静, 周海涛, 牛衍龙, 胡戈, 郭娴, 李兆岩. 有氧运动联合黑果枸杞对高脂膳食大鼠心肌脂代谢某些指标的影响^*[J]. 中国应用生理学杂志, 2020, 36(4): 301-305.