干燥综合征合并特发性肺纤维化miRNA表达谱的变化及生物标志物的研究*

doi:10.12047/j.cjap.6247.2022.051

中国应用生理学杂志 ›› 2022, Vol. 38 ›› Issue (3): 269-272.doi: 10.12047/j.cjap.6247.2022.051

干燥综合征合并特发性肺纤维化miRNA表达谱的变化及生物标志物的研究^*

赵珊, 张虹, 梅坚, 毕丹艳^△

云南省第一人民医院风湿免疫科, 650032

收稿日期:2021-12-06 修回日期:2022-05-28 出版日期:2022-05-28 发布日期:2022-09-05
通讯作者: ^△Tel: 13888439958; E-mail: 82374314@qq.com
基金资助:
^*云南省科技厅-昆明医科大学应用基础研究联合专项资金项目(2017FE467(-115))

Changes of miRNA expression profile and biomarkers in Sjogren's syndrome complicated with idiopathic pulmonary fibrosis

ZHAO Shan, ZHANG Hong, MEI Jian, BI Dan yan

Received:2021-12-06 Revised:2022-05-28 Online:2022-05-28 Published:2022-09-05

摘要/Abstract

摘要： 目的: 探究干燥综合征合并特发性肺纤维化患者血清中miRNA表达谱之间的差异关系。方法: 选择在云南省第一人民医院确诊为干燥综合征的3例患者作为对照组,平均年龄为(55.67±4.78) 岁,病程为(10.67±1.70)月;3例干燥综合征合并特发性肺纤维化患者作为观察组,平均年龄为(57.67±3.68) 岁,病程为(11.00±2.45) 月;6例患者均为女性。两组基本资料没有差异(P＞0.05)。利用芯片检测两组患者血清中miRNA表达谱的差异。通过GO富集分析,筛选出13个免疫细胞功能相关的聚类;表达差异明显的基因集中于免疫调节的信号通路。采用qRT-PCR验证其中5个表达有差异的miRNA。结果: 芯片检测结果共筛选出差异表达基因13个,其中6个miRNA上调:hsa-miR-6740-5p,hsa-miR-4507,hsa-miR-6775-5p,hsa-miR-4281,hsa-miR-4459,hsa-miR-6089,7个miRNA下调:hsa-miR-6873-3p,hsa-miR-4290,hsa-miR-6858-3p,hsa-miR-574-3p,hsa-miR-92b-3p,hsa-miR-3151-3p,hsa-miR-6886-3p。qRT-PCR结果验证了5个最明显的差异miRNA,结果和芯片趋势一致,差异显著,具有统计学意义。结论: 干燥综合征与干燥综合征合并特发性肺纤维化血清中miRNA表达存在差异,其中miR-6886-3p,miR-6873-3p,miR-574-3p,miR-6740-5p和miR-4507特异性和敏感度较高,可能作为干燥综合征特发性肺纤维化区别于原发性干燥综合征的生物标志物。

关键词: 干燥综合征, 特发性肺纤维化, miRNA, 标志物

Key words: Sjogren's syndrome, idiopathic pulmonary fibrosis, miRNA, biomarkers

中图分类号:

R259

赵珊, 张虹, 梅坚, 毕丹艳. 干燥综合征合并特发性肺纤维化miRNA表达谱的变化及生物标志物的研究^*[J]. 中国应用生理学杂志, 2022, 38(3): 269-272.

ZHAO Shan, ZHANG Hong, MEI Jian, BI Dan yan. Changes of miRNA expression profile and biomarkers in Sjogren's syndrome complicated with idiopathic pulmonary fibrosis[J]. CJAP, 2022, 38(3): 269-272.

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干燥综合征合并特发性肺纤维化miRNA表达谱的变化及生物标志物的研究^*

Changes of miRNA expression profile and biomarkers in Sjogren's syndrome complicated with idiopathic pulmonary fibrosis

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