不同方式运动对去负荷大鼠比目鱼肌收缩性能的影响*

doi:10.12047/j.cjap.6273.2022.067

中国应用生理学杂志 ›› 2022, Vol. 38 ›› Issue (4): 356-360.doi: 10.12047/j.cjap.6273.2022.067

不同方式运动对去负荷大鼠比目鱼肌收缩性能的影响^*

胡世亮¹, 杨文超¹, 左鲁玉¹, 蒋强², 张树玲¹, 孙君志^1△

1.成都体育学院运动医学与健康研究所, 四川成都 610000;
2.吉利学院, 四川成都 610000

收稿日期:2022-02-14 修回日期:2022-02-14 出版日期:2022-07-28 发布日期:2022-11-23
通讯作者: ^△Tel: 028-85095371; E-mail: jzhsun@qq.com
基金资助:
^*国家科技支撑计划课题(2012BAK21B01); 四川省科技应用基础研究项目(2019YJ0544); 运动医学四川省重点实验室暨国家体育总局运动医学重点实验室项目(2021-A205)

Effects of different types of exercise on contractile properties and expressions of MuRF1, PGC-1α /FNDC5 in soleus muscle of unloaded rats

HU Shi-liang¹, YANG Wen-chao¹, ZUO Lu-yu¹, JIANG Qiang², ZHANG Shu-ling¹, SUN Jun-zhi^1△

1. Institute of Sports Medicine and Health, Chengdu Sports University, Chengdu 610000;
2. Geely University of China, Chengdu 610000, China

Received:2022-02-14 Revised:2022-02-14 Online:2022-07-28 Published:2022-11-23

摘要/Abstract

摘要： 目的: 探究有氧与抗阻运动对去负荷性肌萎缩大鼠比目鱼肌收缩特性及蛋白MuRF1,PGC-1α和FNDC5表达的影响以及可能的分子生物学机制。方法: 将雄性Wistar大鼠随机分为恢复组(CT)、有氧运动组(A)、抗阻运动组(R)和对照组(C),每组6只。对照组不作任何实验处理,其余3组先进行2周尾部悬吊,而后恢复组安静恢复,有氧组与抗阻组进行2周运动干预。运动方案:有氧组大鼠采用65%最大摄氧量(VO₂max)对应的跑台速度,60 min/d,5 日/周;抗阻组大鼠负重65%最大有意负重(MVCC)爬梯,3次为一组,共5组,每次休息1 min,每组间歇2 min,5 日/周。最后一次运动后禁食24 h,取比目鱼肌观察组织学变化、测试收缩性能并检测MuRF1,PGC-1α和FNDC5表达情况。结果: 与对照组相比,恢复组大鼠体重、比目鱼肌湿重、肌纤维平均横截面积与肌收缩性能都明显降低(P<0.01),PGC-1α/FNDC5表达明显降低(P<0.01)和MuRF1表达明显升高(P<0.01);与恢复组相比,有氧组和抗阻组大鼠体重、比目鱼肌湿重、肌纤维平均横截面积与肌收缩性能都明显升高(P<0.01),PGC-1α/FNDC5表达明显升高(P<0.01)和MuRF1表达明显降低(P<0.01)。与有氧组相比,抗阻组大鼠比目鱼肌PGC-1α表达显著升高(P<0.05)且MuRF1表达显著降低(P<0.05)。结论: 有氧和抗阻运动可明显提高肌收缩性能,上调PGC-1α/FNDC5的表达,抑制MuRF1蛋白表达,表明有氧与抗阻运动改善去负荷性肌萎缩的分子机制可能与PGC-1α和MuRF1蛋白相关。

关键词: 有氧运动, 抗阻运动, 去负荷, 大鼠, 比目鱼肌, 收缩性能

Abstract: Objective: To investigate the effects of aerobic versus resistance exercise on soleus muscle contractile properties and the expressions of MuRF1, PGC-1α and FNDC5 in amyotrophic rats after unloading, and the possible molecular biological mechanisms. Methods: Male Wistar rats were randomly divided into recovery group (CT), aerobic exercise group (A), resistance exercise group (R) and control group (C), with 6 rats in each group. The control group did not receive any treatment. The other three groups underwent tail suspension for 2 weeks, and then the recovery group recovered quietly. The aerobic group and the resistance group underwent a 2-week exercise intervention. Exercise plan: the aerobic group rats were treated with treadmill speed corresponding to 65% maximum oxygen uptake (VO₂max), 60 min/d, 5 d/w; the rats in the resistance group were allowed to climb the ladder with 65% of the maximum voluntary weight-bearing (MVCC) for 3 times, with a total of 5 sets. Each time had a rest of 1 min, with an interval of 2 min among sets, and 5 d/w. Fasting for 24 hours after the last exercise, the soleus muscle samples were collected to observe the histological changes, test the contractility, and detect MuRF1 and PGC-1α and FNDC5 expressions. Results: compared with the control group, the body weight, soleus muscle wet weight, average cross-sectional area of muscle fibers and muscle contractility of the recovery group were decreased significantly(P<0.01), and the expression of MuRF1 was increased significantly(P<0.01). Compared with the recovery group, the body weight, wet weight of soleus muscle, the average cross-sectional area of muscle fiber and muscle contractility of rats in aerobic group and resistance group were increased (P<0.01), the expression of PGC-1α/FNDC5 was increased (P<0.01) and the expression of MuRF1 was decreased significantly (P<0.01). Compared with the aerobic group, the expression of PGC-1α in soleus muscle of rats in the resistance group was increased (P<0.05), while the expression of MuRF1 was decreased (P<0.05). Conclusion: Aerobic and resistance exercise can significantly improve muscle contractility, upregulate the expression of PGC-1α/FNDC5, and inhibit the expression of MuRF1, indicating that the molecular mechanisms of aerobic and resistance exercise to improve unloaded muscular atrophy may be related to PGC-1α and MuRF1.

Key words: aerobics, resistance training, unloaded, rats, soleus, contractile properties

中图分类号:

G804.2

胡世亮, 杨文超, 左鲁玉, 蒋强, 张树玲, 孙君志. 不同方式运动对去负荷大鼠比目鱼肌收缩性能的影响^*[J]. 中国应用生理学杂志, 2022, 38(4): 356-360.

HU Shi-liang, YANG Wen-chao, ZUO Lu-yu, JIANG Qiang, ZHANG Shu-ling, SUN Jun-zhi. Effects of different types of exercise on contractile properties and expressions of MuRF1, PGC-1α /FNDC5 in soleus muscle of unloaded rats[J]. CJAP, 2022, 38(4): 356-360.

参考文献

[1] 于亮, 王瑞元, 陈晓萍. 加压运动对去负荷肌萎缩的影响及机制研究进展[J]. 生理科学进展, 2016, 47(3): 227-230.
[2] Vechetti-Junior IJ, Bertaglia RS, Fernandez GJ, et al. Aerobic exercise recovers disuse-induced atrophy through the stimulus of the LRP130/PGC-1α complex in aged rats[J]. J Gerontol A Biol Sci Med Sci, 2016, 71(5): 601-609.
[3] Zhong X, Sun X, Shan M, et al. The production, detection, and origin of irisin and its effect on bone cells[J]. Int J Biol Macromol, 2021, 178: 316-324.
[4] Branden LN, Toshinori Y, Rafael D, et al. Alterations in renin-angiotensin receptors are not responsible for exercise preconditioning of skeletal muscle fibers[J]. Sports Med Health Sci, 2021, 3(3): 148-156.
[5] Akagawa M, Miyakoshi N, Kasukawa Y, et al. Effects of activated vitamin D, alfacalcidol, and low-intensity aerobic exercise on osteopenia and muscle atrophy in type 2 diabetes mellitus model rats[J]. PLoS One, 2018, 13(10): e0204857.
[6] Zhang Y, Pan X, Sun Y, et al. The molecular mechanisms and prevention principles of muscle atrophy in aging[J]. Muscle Atrophy, 2018: 347-368.
[7] Liu Y, Guo C, Liu S, et al. Eight weeks of high-intensity interval static strength training improves skeletal muscle atrophy and motor function in aged rats via the PGC-1α/FNDC5/UCP1 pathway[J]. Clin Interv Aging, 2021, 16: 811-821.
[8] Farsani ZH, Banitalebi E, Faramarzi M, et al. Effects of different intensities of strength and endurance training on some osteometabolic miRNAs, Runx2 and PPARγ in bone marrow of old male wistar rats[J]. Mol Biol Rep, 2019, 46(2): 2513-2521.
[9] Moreira VM, Almeida D, da Silva Franco CC, et al. Moderate exercise training since adolescence reduces Walker 256 tumour growth in adult rats[J]. J Physiol, 2019, 597(15): 3905-3925.
[10] Singulani MP, Stringhetta-Garcia CT, Santos LF, et al. Effects of strength training on osteogenic differentiation and bone strength in aging female Wistar rats[J]. Sci Rep, 2017, 7(1): 1-11.
[11] 高放, 余志斌. 去负荷比目鱼肌高频强直收缩疲劳性的动态变化[J]. 第四军医大学学报, 2004(20): 1831-1833.
[12] 汪军, 周越, 孙君志, 等. 质疑与思考:运动生理学研究的十个问题[J]. 成都体育学院学报, 2021, 47(1): 118-124.
[13] 史华彩, 陈睿, 佘燕玲, 等. Atrolnc-1在制动诱导小鼠后肢肌萎缩中的作用研究[J]. 中国应用生理学杂志, 2021, 37(5): 566-570.
[14] Theilen NT, Jeremic N, Weber GJ, et al. Exercise preconditioning diminishes skeletal muscle atrophy after hindlimb suspension in mice[J]. J Appl Physiol(1985), 2018, 125(4): 999-1010.
[15] Akagawa M, Miyakoshi N, Kasukawa Y, et al. Effects of activated vitamin D, alfacalcidol, and low-intensity aerobic exercise on osteopenia and muscle atrophy in type 2 diabetes mellitus model rats[J]. PloS one, 2018, 13(10): e0204857.
[16] Buso A, Comelli M, Picco R, et al. Mitochondrial adaptations in elderly and young men skeletal muscle following 2 weeks of bed rest and rehabilitation[J]. Front Physiol, 2019, 10: e474.
[17] MacNeil LG, Glover E, Bergstra TG, et al. The order of exercise during concurrent training for rehabilitation does not alter acute genetic expression, mitochondrial enzyme activity or improvements in muscle function[J]. PLoS One, 2014, 9(10): e109189.
[18] 苏艳红, 袁乾坤, 肖蓉, 等. 抗阻训练对增龄大鼠骨骼肌线粒体功能的影响[J]. 中国应用生理学杂志, 2020, 36(2): 165-170.
[19] Hood DA, Tryon LD, Vainshtein A, et al. Exercise and the regulation of mitochondrial turnover[J]. Prog Mol Biol Transl Sci, 2015, 135: 99-127.
[20] Reza MM, Subramaniyam N, Sim CM, et al. Irisin is a pro-myogenic factor that induces skeletal muscle hypertrophy and rescues denervation-induced atrophy[J]. Nat Commun, 2017, 8(1): 1-17.

不同方式运动对去负荷大鼠比目鱼肌收缩性能的影响^*

Effects of different types of exercise on contractile properties and expressions of MuRF1, PGC-1α /FNDC5 in soleus muscle of unloaded rats

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