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CJAP ›› 2017, Vol. 33 ›› Issue (6): 488-492.doi: 10.12047/j.cjap.5601.2017.116

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Expressions of synaptophysin and BDNF/Trk-B in cerebellum of APPswe/PS1dE9 transgenic mice

DONG Xue-fan, WANG Ting, LI Tian, JIAO Juan-juan, QU Xue-song, QI Jin-shun, YANG Wei   

  1. Department of Physiology, Key Laboratory of Cellular Physiology, Ministry of Education, Shanxi Medical University, Taiyuan 030001, China
  • Received:2016-11-28 Revised:2017-05-23 Online:2017-11-28 Published:2018-06-19
  • Contact: 杨威,Tel:13293910485;E-mail:vividmail@163.com E-mail:vividmail@163.com
  • Supported by:
    山西省回国留学人员科研资助项目(2016060)

Abstract: Objective: To observe the expressions of synaptophysin and BDNF/Trk-B in cerebellum of APPswe/PS1dE9 transgenic mice.Methods: The healthy 9-month old APP/PS1 male mice (n1) and the same wild type male mice(n2) were divided into two groups, APP/PS1 group and wild-type(WT) group. The expressions of synaptophysin and brain-derived neurotrophic factor/tyrosine kinase B (BDNF/Trk-B) in cerebellum were determined by Western blot (n1=6; n2=6) and immunohistochemical(n1=4; n2=4).The possible synaptic changes in APP/PS1 group were observed by using electron microscopy.Results: Compared with WT group, the expressions of synaptophsin and BDNF/Trk-B in cerebellum were decreased in APP/PS1 group. Increased width of the synaptic cleft and decreased thickness of postsynaptic density were also observed.Conclusion: In APP/PS1 group, expressions of synaptophsin and BDNF/Trk-B in cerebellum were decreased; changes in ultrastructure of synapses seemed to be widespread alterations. These findings suggest a possible association between expression of BDNF/TrkB and synaptic plasticity in AD cerebellum.

Key words: APP/PS1 transgenic mouse, cerebellum, synaptophysin, BDNF, Trk-B, synapse

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