Changes of lipid levels in mice with hypoxic pulmonary arterial hypertension

doi:10.13459/j.cnki.cjap.2016.05.020

Abstract

Abstract: Objective: To observe the changes of lipid levels in mice with pulmonary hypertension induced by hypoxia. Methods: The animal model of hypoxic pulmonary hypertension was established by exposing the mice to isobaric hypoxic chamberfor 3 weeks (23 h/d, regular chow feed). Twenty male C57BL/6 mice were randomlydivided into normoxia group and hypoxia group (n=10). The concentrations of total cholesterol, low density lipoprotein (LDL) and high density lipoprotein (HDL) in plasma were detected by Elisa method.The mRNA levels of HMG-CoAreductase (HMGCR), low density lipoprotein receptor (LDLR), scavenger receptor class B1 (SR-B1), and sterol regulatory element-binding factor-2 (SREBF2) in liverwere measured by real-time PCR. Results: ① The right ventricular systolic pressure (RVSP) and the weight ratio of right ventricle (RV) to left ventricle plus septum (LV+S) of hypoxia group were significantly higher than those of normoxia group (P<0.05).② The concentrations of HDL and HDL/LDL in plasma were significantly higher in hypoxia group, compared with normoxia group (P<0.05).③The mRNA levels of LDLR and SR-B1in liver were significantly down-regulated in hypoxia group(P<0.05).④RVSP were significantly negative correlated with HDL/LDL, the gene expression of LDLR and SR-B1 (P<0.05). Conclusion: Abnormal lipid metabolism participates in the pathological proceeding of pulmonary hypertension induced by hypoxia.

Key words: lipid metabolism, pulmonary hypertension, hypoxia, mice

FAN Feng-yun, SHEN Wan-ting, ZHENG Qing-qing, GAO Yuan, HU Kai-yuan, FAN Xiao-fang, MAO Sun-zhong, GONG Yong-sheng. Changes of lipid levels in mice with hypoxic pulmonary arterial hypertension[J]. CJAP, 2016, 32(5): 463-465.

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