Effects of cold exposure on estrous cycle of female C57BL/6 mice

doi:10.12047/j.cjap.5876.2019.098

CJAP ›› 2019, Vol. 35 ›› Issue (5): 454-456.doi: 10.12047/j.cjap.5876.2019.098

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Effects of cold exposure on estrous cycle of female C57BL/6 mice

ZHAO Hai^1,2, LI Xi²⁺, ZHANG Li², WU Shuai², ZHANG Yong-qiang², LI Jun³, ZHANG Ge-xiang^1△, YANG Dan-feng^2△

1. School of Public Health, Lanzhou University, Lanzhou 730000;
2. Institute of Environmental and Operational Medicine, Academy of Military Medical Sciences, Academy of Military Sciences, Tianjin 300050;
3. Chinese PLA 32110, Heihe, China

Received:2019-05-09 Online:2019-09-28 Published:2020-01-02

Abstract

Abstract: Objective: To study the effects of cold exposure on estrous cycle of female C57BL/6 mice. Methods: Twelve healthy female C57BL/6 mice were randomly divided into two groups: control group and cold exposure group, 6 in each group. Cold exposure group was exposed to 4℃, 4 h per day, while control group stayed in normal conditions. Vaginal smears were used to observe the estrous cycle. After 2 weeks, blood and uteri were collected from each mouse after anesthetized and weighted. Serum levels of estradiol(E2), follicle-stimulating hormone(FSH), luteinizing hormone(LH), prolactin(Prl) and progesterone(P) were determined by using mouse ELISA kits. The uterus and ovary pathological slices were prepared to observe the structural changes. Results: Compared with the control group, body weight gain showed no significant differences (P＞0.05). The cold group had significant lower coefficients of uterus and the diestrus phase was significantly increased after cold exposure (P＜0.01). Serum level of FSH in cold group was higher and Prl was lower significantly (P＜0.01). Pathological examination of uterus and ovary showed that uterine glands of cold group were expanded and the amount of follicles was decreased significantly. Conclusion: Cold exposure might increase mouse estrous cycle and affect their reproductive function.

Key words: cold exposure, estrous cycle, C57BL/6 mice, uterus, ovary

ZHAO Hai, LI Xi, ZHANG Li, WU Shuai, ZHANG Yong-qiang, LI Jun, ZHANG Ge-xiang, YANG Dan-feng. Effects of cold exposure on estrous cycle of female C57BL/6 mice[J]. CJAP, 2019, 35(5): 454-456.

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Effects of cold exposure on estrous cycle of female C57BL/6 mice

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