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CJAP ›› 2016, Vol. 32 ›› Issue (3): 234-237.doi: 10.13459/j.cnki.cjap.2016.03.012

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Effects of acute cold exposure on the expression of AQP-1 and AQP-5 in rat lung tissues

LIN Yang-sheng1, ZHANG Li1, YANG Hua2, ZHANG Ying1, LI Xi1, ZHANG Yong-qiang1, YANG Dan-feng1   

  1. 1. Institute of Health and Environmental Medicine,Academy of Military Medical Sciences, Tianjin 300050;
    2. The 222th Hospital of Chinese People's Liberary Army, Jilin 132000, China
  • Received:2015-12-21 Revised:2016-02-15 Online:2016-05-28 Published:2018-06-12
  • Contact: 杨丹凤,Tel:022-84655058;E-mail:fengdyd@126.com E-mail:fengdyd@126.com
  • Supported by:
    杨丹凤,Tel:022-84655058;E-mail:fengdyd@126.com

Abstract: Objective:To study the effect of acute cold exposure on the expression of aquaporin-1 (AQP-1) and AQP-5 in lung tissues and the changes of ultrastructural pathological changes after cold exposure in rats. Methods:Twelve male Wistar rats were randomly divided into control group (23±2)℃ and cold (-25℃) exposure group, the exposure time was 2 h. Rectal temperatures of the rats were measured immediately after cold exposure. The ultrastructural pathological changes of pulmonary tissue were observed by transmission electronic microscope. The mRNA expression of AQP-1 and AQP-5 was measured by RT-PCR. The protein expression of AQP-1 and AQP-5 was measured by Western blot. Results:The body core temperatures(28.07±4.15)℃ of the cold exposure group were decreased significantly compared with the control group(37.33±0.25)℃ (P<0.05). In acute cold exposure group, the main pathological changes of pulmonary ulstructure included pulmoary epithelial basement membrane thickening, and karyopyknosis of AT-I cells, and vacuolization on the cytoplasm of (AT-Ⅱ) cells. After acute cold exposure, the levels of both mRNA and potein expressions of AQP-5 were decreased significantly (P<0.05) compared with those in the control group. while AQP-1 expression level showed no statistical significance between control group and cold exposure group. Conclusion:There might be some cause and effect relationship between lung tissue damage by cold exposure and the levels of mRNA and potein expressions of AQP-5 decreased after acute cold exposure.

Key words: acute cold exposure, lung, aquaporin, rat

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