Home  About Journal Instructions for Authors Editorial Board Subscribe Advertisement Messages Board Contact Us 中文

CJAP ›› 2019, Vol. 35 ›› Issue (5): 454-456.doi: 10.12047/j.cjap.5876.2019.098

• ORIGINAL ARTICLES • Previous Articles     Next Articles

Effects of cold exposure on estrous cycle of female C57BL/6 mice

ZHAO Hai1,2, LI Xi2+, ZHANG Li2, WU Shuai2, ZHANG Yong-qiang2, LI Jun3, ZHANG Ge-xiang1△, YANG Dan-feng2△   

  1. 1. School of Public Health, Lanzhou University, Lanzhou 730000;
    2. Institute of Environmental and Operational Medicine, Academy of Military Medical Sciences, Academy of Military Sciences, Tianjin 300050;
    3. Chinese PLA 32110, Heihe, China
  • Received:2019-05-09 Online:2019-09-28 Published:2020-01-02

Abstract: Objective: To study the effects of cold exposure on estrous cycle of female C57BL/6 mice. Methods: Twelve healthy female C57BL/6 mice were randomly divided into two groups: control group and cold exposure group, 6 in each group. Cold exposure group was exposed to 4℃, 4 h per day, while control group stayed in normal conditions. Vaginal smears were used to observe the estrous cycle. After 2 weeks, blood and uteri were collected from each mouse after anesthetized and weighted. Serum levels of estradiol(E2), follicle-stimulating hormone(FSH), luteinizing hormone(LH), prolactin(Prl) and progesterone(P) were determined by using mouse ELISA kits. The uterus and ovary pathological slices were prepared to observe the structural changes. Results: Compared with the control group, body weight gain showed no significant differences (P>0.05). The cold group had significant lower coefficients of uterus and the diestrus phase was significantly increased after cold exposure (P<0.01). Serum level of FSH in cold group was higher and Prl was lower significantly (P<0.01). Pathological examination of uterus and ovary showed that uterine glands of cold group were expanded and the amount of follicles was decreased significantly. Conclusion: Cold exposure might increase mouse estrous cycle and affect their reproductive function.

Key words: cold exposure, estrous cycle, C57BL/6 mice, uterus, ovary

Contact Email:editor@cjap.ac.cn
Copyright © 2015 CJAP, All Rights Reserved.
Powered by Beijing Magtech Co. Ltd