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CJAP ›› 2021, Vol. 37 ›› Issue (5): 495-499.doi: 10.12047/j.cjap.6091.2021.060

• ORIGINAL ARTICLES • Previous Articles     Next Articles

Effects of Betulinic acid on apoptosis in human gastric cancer SGC-7901 cells

DING Lu1, SHAO Shu-li1,2, HE Meng-qi1, HUANG Xin 1,2, ZHANG Wei-wei1,2, ZHANG Zhen-zhu1,2   

  1. 1. College of Life Science and Agriculture and Forestry;
    2. Heilongjiang Provincial Key Laboratory of Resistance Gene Engineering and Protection of Biodiversity in Cold Areas, Qiqihar University, Qiqihaer 161000, China
  • Received:2020-05-14 Revised:2021-02-02 Online:2021-09-28 Published:2021-11-24

Abstract: Objective: To investigate the effects of betulinic acid on apoptosis of human gastric cancer SGC-7901 cells. Methods: The human gastric cancer SGC-7901cells were divided in to 4 groups, and each group was set with 3 replicates. The SGC-7901cells in control group were not treated with betulinic acid; the other 3 experimental groups were treated with betulinic acid at the concentrations of 10, 20 and 30 mg/L, respectively; each group was incubated in a 5% carbon dioxide incubator for 48 h. Laser confocal microscope was used to observe morphological changes of SGC-7901 cells; Flow cytometry was applied to determine apoptosis rate and mitochondrial membrane potential. The mRNA and protein levels of Bcl-2, Bax and Caspase-3 were also detected by qRT-PCR and western blot respectively. Results: Compared with the control group, SGC-7901 cells in the treated group at final concentrations of 10, 20 and 30 mg/L shrinked, appeared apoptosis body along with nuclear splitting. The percentage of cells in early and advanced period of apoptosis were markedly increased (P<0.05 or P<0.01), mitochondrial membrane potential was obviously reduced (P<0.05 or P<0.01). qRT-PCR and western blot analysis showed that the mRNA and protein expressions of Bax and Caspase-3 were increased significantly (P<0.01), while the expressions of Bcl-2 were decreased significantly (P<0.01). Conclusion: Within a certain range of concentrations, betulinic acid induces cell apoptosis by regulating the expression of Bcl-2, Bax and Caspase-3 in human gastric cancer.

Key words: betulinic acid, human gastric carcinoma SGC-7901 cells, cell apoptosis

CLC Number: 

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