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| Effect of BRAF Gene Mutation on Colon Cancer and Associated With Gastric Cancer |
| ZHOU Haiyang, WEI Xueming, ZHENG Aimin, DAI Dajiang |
| Department of General Surgery, Air Force General Hospital, Beijing 100142, China |
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Abstract Objective To investigate the association between BRAF mutation and colon cancer with stomach cancer, and between BRAF mutation and gene expression of PRDX6 and PRDX1, and to analyze the role of PRDX1 and PRDX6 gene in colon cancer with other primary cancers.Methods A total of 47 patients with colon cancer complicated by gastric cancer samples were collected. Mutation of BRAF and MLH1 genes were detected by PNAClampTM kit, and chi-square test was used to compare the relationship between mutations and related characteristics. Expressions of PRDX1 and PRDX6 were quantified by qPCR and Western blot in samples with and without BRAF mutation. The plasmid with the BRAF mutation was transferred into human HCT-116 colon cancer cells to investigate the expression levels of PRDX1 and PRDX6.Results BRAF mutation had no correlation with age, cancer location, multiple cancer time intervals and the number of cancer or family history of cancer; while MLH1 mutation had correlation with cancer multiple time intervals and the number of cancer and family cancer history (P<0.05). PRDX1 and PRDX6 expression levels were correlated with the degree of the BRAF mutation and the number of primary cancer in colon cancer (P<0.05). PRDX1 and PRDX6 might be the target of BRAF mutation inhibition.Conclusion BRAF mutation rate in colon cancer complicated by gastric cancer is relatively low in the Chinese population. When using BRAF mutation as a marker for detection, other genes such as MLH1 gene mutation may be as auxiliary. BRAF mutation inhibits PRDX1 and PRDX6, which in turn may affect the number of primary cancer.
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Published: 14 September 2016
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2016, Vol. 34 
