Objective To prepare and express the Rta recombinant antigen of Epstein-Barr virus and to establish an ELISA method in order to facilitate the screening of nasopharyngeal carcinoma (NPC).Methods The target gene BRLF1 which coded for Rta was ampliflied by PCR with a specific primer. The recombinant vector pPICZαA-BRLF1 was electronically transformed into pichia pastoris GS115 to express Rta protein so as to screen the His+Mut+ phenotype transformant before proteins were purified and the immunogenicity of the product was proved by Western blot. ELISA was established with the IgG/Rta antibody and was applied to the detection of 300 patients with NPC and 200 healthy people to assess the capability of detection.Results The pPICZαA-BRLF1 recombinant plasmid was successfully constructed and the target protein was secreted and expressed with high-performance (50%). Protein electrophoresis demonstrated a corresponding band in 66 KDa. Western blot showed a good immune-competence with the IgG monoclonal antibody of Rta. The Rta recombinant antigen was used for establishment of the ELISA method. The positive rate of this method was significantly higher than that of IgA/VCA Kit for screening NPC (P<0.01). Conclusion A eukaryotic expression vector of the Rta recombinant antigen of Epstein-Barr virus has been constructed that can ensure the high-performance of foreign proteins with good immunogenicity for the development of IgG/Rta ELISA methods. The detection capability can be improved by combining other antigens and this method is applicable to large-scale screening and diagnosis of NPC.

Objective To investigate the protective effect of branched-chain amino acid(BCAA)on muscle atrophy in weightlessness-simulated rats.Methods Weightlessness was simulated by a tail-suspended model in rats. SD rats were randomly divided into 3 groups: ground control group (GC), tail-suspended control group (TC) and tail-suspended with BCAA intervention group (TBI).All the rats were successively fed with normal diets. The rats in GC and TC groups received pure water, and those in TBI group were given pure water and intervened with BCAA solution via intragastric administration respectively. The biochemical indices of serum and soleus (SOL) were detected after 4 weeks.Results Compared with GC group, the muscle wet weight(MWW) of and the ratio of MWW to body weight of rats in the TC group were significantly decreased(P<0.01),the level of serum BUN and Ub was significantly increased(P<0.05),the level of serum IGF-1、TESTO、SOD、 and GSH-Px was significantly decreased(P<0.05), and the expression of atrogin-1 protein in SOL decreased by 32%. Compared with TC group, the muscle wet weight(MWW) and the ratio of MWW to body weight were significantly increased (P<0.01) in TBI group,so was the serum level of IGF-1、TESTO、Ub, SOD and GSH-Px,,but the serum BUN and the expression of atrogin-1 protein in SOL were decreased,while the level of SOD and GSH-Px was increased significantly(P<0.05). (P<0.05).Conclusion BCAA may promote the synthesis of SOL protein, but inhibit its breakdown to some extent,alleviating SOL atrophy in tail-suspended rats. The mechanism potentially lies in facilitating the secretion of anabolic hormone and antioxidation ability,and inhibiting the expression of atrogin-1 protein.

Objective To evaluate the effect of quercetin on axonal growth and functional recovery in mice after spinal cord injury.Methods Twenty-four C57BL/6 female mice were randomly and evenly divided into two groups. Complete compression injury at the spinal cords of T10 was induced in both groups . The mice in therapeutic group were administered with quercetin (50 mg/kg) while control group received the same dose of saline by intraperitoneal injection at 30min before injury, and immediately after injury, and then administered once a day from 1 to 10 days post-injury. The mice in control group received the same dose of saline with an equally long duration. Biotinylated dextran amine (BDA) was injected into the sensorimotor cortex at 14 days post-injury to trace the corticospinal tract (CST). Counterstaining was performed with glial fibrillary acidic protein (GFAP) to label the lesion boundary. The CST retraction and pullulation were observed under a fluorescent microscope. BMS was performed to assess motor functional recovery at 1, 3, 7, 14 and 28 days post-injury.Results Compared with control group, the ratio of total labeled sprouts/axons was significantly increased, the ratio of total labeled retraction bulbs/axons decreased,and the distance of the bundle to lesion border decreased after quercetin treatment at 28 days post-injury. The BMS scores were also significantly improved in therapeutic group (P<0.01).Conclusion Quercetin can promote axonal pullualtion, prevent axonal retraction and improve motor functional recovery after spinal cord injury in mice.

Objective To investigate the application of ambulatory blood pressure monitoring(ABPM) to medical evaluation of aircrew with early hypertension.Methods According to the 24-hour mean value of ambulatory blood pressure, 32 aircrews with office hypertension were divided into ABPM hypertension group and white coat hypertension (WCH) group, with 30 healthy male pilots as the control group.Results 17 cases (53.13%) were diagnosed with grade 1 hypertension by ABPM and 15 cases(46.88%) with white coat hypertension. Night systolic blood pressure fall rate (nSBPF) and night diastolic blood pressure fall rate (nDBPF) were similar in these three groups. There was statistically significant difference between the three groups in 24 h systolic blood pressure load (24 h SBPL) and 24h diastolic blood pressure load (24 h DBPL).Conclusion ABPM hypertension group and WCH group can maintain normal the circadian rhythm. 24 h SBPL and 24 h DBPL are much more sensitive indexes for classifying the three groups. ABPM can be used as an important reference index of early diagnosis,treatment and medical evaluation of aircrews with hypertension.

Objective To investigate military pilots' self-medication with coldrex and t propose practical and effective countermeasures.Methods A total of 2105 military pilots recuperating in our sanatorium were surveyed with questionnaires.Results The pilots who had self-medication with coldrex accounted for 74.96% of the total. Those who had self-medication with coldrex within 24 hours before flight and felt drowsy at work accounted for 0.10%,those who had self-medication with coldrex despite their knowledge that self-medication was prohibited during flight preparation accounted for 1.19%, those who took coldrex before flight because of their ignorance of related medication rules accounted for 6.32%, and those who tood the drug without any knowledge of the side-effect of coldrex accounted for 12.49%. 92.31% of the 13 types of coldrex taken by pilots were antihistamine drugs with obvious sedative effects, such as chlorpheniramine maleate and diphenhydramine hydrochloride .Conclusion Military pilots' self-medication with coldrex is currently common, posing a great risk to flight safety. Carrying out Education on medication safety should be carried out among pilots and handbooks on safe medication should be published for them to strengthen pilots' awareness of self-protection and to ensure flight safety.

Objective To investigate the knowledge and education of sexual health among hygienic sergeantsso as to provide evidence better education on sexual health among PAPs at the grass-roots level.Methods A survey about sexual and reproductive health was conducted among 207 medical sergeants in one college of PAP in 2014. Results The pencentage of hygienic sergeants who expressed a need for sexual and reproductive health knowledge was 56.2%. The main sources of sexual and reproductive health knowledge were books (62.2%), followed by health education lectures (53.2%) and class instructions (51.2%). Lectures on sexual health were their favorite (74.6%). They were eager for some sexual knowledge, but only 5% of the units at the grass-roots level offered sexual education.Conclusion Hygienic sergeants generally hope for sexual health education, but efforts of education at the grass-roots level can hardly meet their requirements. More efforts are needed to make education on sexual and reproductive health more accessible to servicemen.

Objective To investigate the effect of exogenous dextranase (Dex) combined with sodium fluoride (NaF) on the formation of multi-species biofilms.Methods A model of multi-species biofilms was established in vitro. The pH of inchoate biofilms was detected by placing the tip of a pH electrode into the matrix of the biofilms. The number of viable cells in inchoate biofilms was measured by colony count.Results 1. Dextranase had no effect on the pH of inchoate biofilms, but NaF at each tested concentration inhibited acid genesis of biofilms,which was not affected by Dex. 2. All the test agents significantly reduced viable cells on glass slides and 1U/mL ml Dex plus 80μg/mL ml NaF had synergistic effects on the formation of early biofilms.Conclusion Dextranase and sodium fluoride of low concentrations may have synergistic effects against the formation of multi-species biofilms.

Objective To find out about the prevalence of abnormal eruption of mandible wisdom teeth and the incidence of pericoronitis in some troops of PLA so as to provide evidence for intervention .Methods Oral examinations were given to 4372 servicemen in the army and 2936 in the navy from Guangzhou Military Command. The prevalence of abnormal eruption of mandible wisdom teeth and the incidence of pericoronitis were investigated and analyzed.Results The survey Results showed that the prevalence of abnormal mandible wisdom teeth among these servicemen was 37.3%. The incidence of pericoronitis was 25.7%. The absence rate associated with pericoronitis was 9.7%. Only 8.959.0% of these servicemen were willing to have their abnormal wisdom teeth pulled out.Conclusion The prevalence of abnormal mandible wisdom teeth and incidence of pericoronitis are high among officers and soldiers in some troops, impacting the routines of troops. Servicemen should be better informed of these diseases and three-stage measures of prevention should be taken.

Objective To observe the impact of Porphyromonas gingivalis on the expression of nitric oxide synthase in pulmonary epithelial cells infected with influenza virus .Methods Pulmonary epithelial cells were infected with 2 MOIs (multiplicity of infection) of H1N1 or different MOIs of Porphyromonas gingivalis before being divided into groups A-E. Nitric oxide (NO) production was assessed by measuring the accumulation of nitrite in culture supernatants. The level of inducible nitricoxide synthase (iNOS) was detected by Western blot .Results NO production increased in each group at 4h, 8h, 12h, 24h. The level of NO production in group E at 4h, 8h, 12h, 24h was 41.404 3.079, 78.945 8h, 1, 592.9838h, 12, 2360.660h, and 12h umol/L, respectively, and NO production at 8h, 12h, 24h was significantly higher than that in control group (P<0.05). The expression of iNOS in pulmonary epithelial cells infected with Influenza virus was more significantly promoted by Porphyromonas gingivalis at 12h, 24h, and than in control group (P<0.05).Conclusion Porphyromonas gingivalis may increase the expression of iNOS, thus promoting the synthesis of NO.

Objective To investigate the heart rate variability (HRV) of military pilots transferring to another airport.Methods Thirty-six military pilots who had transferred to another airport (Group 1) and another thirty-six military pilots who stayed put (Group 2) were randomly selected. ECG of both groups was recorded for two consecutive hours to measure and analyze their heart rate variability.Results The rate of HRV abnormity of pilots in Group 1 was significantly higher than that in Group 2 (P<0.01). SDNN , rMSSD, TP, LF, HF of pilots in Group 1 were all significantly lower than those in Group 2(P<0.01), but the LF/HF of pilots in Group 1 was significantly higher than that of Group 2 (P<0.05).Conclusion The heart rate variability of military pilots transferring to another airport can be decreased significantly. Some effective measures should be taken to solve this problem.

Objective To analyze the water quality and sanitary conditions of swimming pools in Tianjin between 2011 and 2014 in order to ensure the health of swimmers.Methods Water samples were tested according to Test Methods of Hygienic Standards for Public Places, GB/T 18204-2000"GB/T 18204-2000,and evaluations were conducted based on Health Standards for Swimming Pools, GB 9667-1996"GB 9667-1996.Results A total of 260 water samples of swimming pools were tested between 2011 and 2014, and the total qualified rate was 81.2%. The unqualified indexes mainly involved urea and free residual chlorine. The difference in qualified rates between various swimming pools was of statistical significance. The qualified rate of water quality of swimming pools in hotels was the highest, followed by school swimming pools and public swimming pools.Conclusion The water quality of swimming pools in Tianjin has been improved year by year, but health survaillance of public swimming pools should be strengthened, water sterilization enhanced and water replaced regularly.

Objective To evaluate the therapeutic effect of hyperbaric oxygen combined with traditional Chinese medicine and creatine phosphate sodium on carbon monoxide poisoning.Methods, A total of 120 carbon monoxide poisoning patients in our hospital from June 2012 to December 2014 were divided into the observation group (n=60) and control group (n=60). The patients of control group received hyperbaric oxygen therapy, while those of observation group were treated with traditional Chinese medicine and creatine phosphate sodium plus hyperbaric oxygen therapy. The HDS score, P300 latency, neurologic impairment score and myocardial injury markers were compared between the two groups before and after treatment.Results After treatment, the HDS score of the observation group was significantly higher than that of the control group (P＜0.05), but the latency of P300 was significantly shortened (P＜0.05). NIHSS score and serum cTNI, IMA, CK-MB levels in the observation group were significantly lower than those in the control group (P＜0.05). The therapeutic effect of observation group was significantly better than that of control group (P＜0.05).Conclusion Hyperbaric oxygen combined with traditional Chinese medicine and creatine phosphate sodium can effectively protect myocardium and neurons during the treatment of patients with carbon monoxide poisoning. The therapeutic effect is significantly better than that of hyperbaric oxygen therapy alone.

Objective To explore the association of procalcitonin(PCT), C-reactive protein(CRP) and lactic acid with recent prognosis in elderly patients of community-acquired pneumonia(CAP).Methods A total of 108 cases of elderly CAP patients in a hospital were selected as subbjects between March 2012 and June 2015. They were divided into the severe group(n=41)and non-severe group(n=67),or alternatively the death group(n=23)and survival group(n=85).CURB-65 scores were calculated within 24 h after admission.Chemiluminescence method and immune turbidimetric method were used respectively to determine serum PCT and CRP levels, and the arterial blood lactic acids were detected using the ABL800 blood gas analyzer.Results The levels of PCT〔(8.87±4.65) ng/ml〕,CRP〔(28.03±11.61) mg/L〕, arterial blood lactate〔(5.40±2.23) mmol/L〕 and CURB-65 scores(4.51±1.39) in the severe group were significantly higher than those in the non-severe group. The levels of PCT〔(9.62±4.36) ng/ml〕,CRP〔(32.90±12.03) mg/L〕, arterial blood lactate〔(6.68±1.81) mmol/L〕 and CURB-65 scores(4.81±1.16)in the death group were significantly higher than those in the survival group. Serum PCT, CRP, arterial blood lactate levels were positively correlated with CURB-65 scores (r=0.703,r=0.631,r=0.544,P<0.05).Conclusion PCT, CRP and lactic acid are of diagnostic value for the severity of elderly patients of CAP,.High levels of PCT , CRP and lactic acid suggest a poor prognosis of CAP.

Objective To investigate the effect of 8-week basic military training(BMT) on army recruits' 'red blood cells and other related indicators.Methods Data were from 50 male recruits of Xinjiang Frontier Armed Police Forces in 2015. The hemogram and serum ferritin of the subjects were measured and recorded at the beginning and end of 8-week BMT respectively.Results After 8-week BMT, hemoglobin concentration(HGB), red blood cell count(RBC), and serum ferritin decreased significantly(P＜0.05,P＜0.01).Conclusion For the army recruits , 8-week BMT can lead to sports anemia, and iron deficiency may be the leading cause.

Objective To explore the relationships between hardiness,general self-efficacy,social support and subjective well-being among soldiers who participated in a large-scale military exercise so as to provide evidence for promoting the hardiness and mental health of soldiers who participated in a large-scale military exercise.Methods The Hardiness Scale for Chinese Adults, General Self-Efficacy Scale (GSES),Perceived Social Support Scale(PSSS) and Subjective Well-being Schedule (GWB) were used among 320 servicemen during a field military exercise. Statistical package SPSS version 18.0 was used for the analysis.Results A total of 309 valid questionnaires were analyzed. The level of hardiness was significantly different between positive and negative emotions(Z=-3.430,P<=0.001). The level of hardiness of soldiers showed significantly positive correlations with general self-efficacy(r=0.541,P<0.01), perceived social support(r=0.266,P<0.01), life satisfaction(r=0.319,P<0.01) and positive emotions(r=0.342,P<0.01). but was negatively correlated with negative emotions(r=-0.144,P<0.05).Conclusion Hardiness is significantly related to general self-efficacy,social support and subjective well-being. Positive and negative emotions alike have some effects on hardiness.

Objective To explore the clinical effect of percutaneous renal lithotripsy (tubeless-PCNL) with a micro channel of indwelling ureteral stents on the treatment of ureteral calculi.Methods The clinical diagnosis and treatment of 120 cases of ureteral calculi patients were analyzed retrospectively in a hospital between 2012 and 2014. Among these cases, 57 received traditional PCNL surgery (traditional group), and 63 received tubeless-PCNL treatment (tubeless-PCNL group). Indications for surgery, postoperative recovery and complications were statistically analyzed between the two groups.Results The operation time(59.3?.2)min, postoperative 24h VAS score(2.40?.81),analgesic usage(6.35%),length of hospital stay(6.3?.5)d of patients in tubeless-PCNL group were significantly lower than those in traditional group, and the difference was statistically significant (P<0.05,P<0.01). There was no significant difference in hemoglobin (P>0.05), HCT or fever between the two groups . The incidence of perirenal effusion(11.1%),postoperative urgent urination(3.2%),hematuria(7.9%),low back pain(4.8%) of patients in tubeless-PCNL group was significantly lower than in traditional group, and the difference was statistically significant(P< 0.05).Conclusion Tubeless-PCNL treatment of upper ureteral calculi has the advantages of a short operation time, quick recovery, low degree of pain and low complication rate.

Objective To investigate the incidence of hypertension among elders and the influencing factors in communities in Jinzhou, Liaoning Province, so as to provide some reference for community prevention of senile hypertension.Methods Between February and December of 2014, 300 elders in Linghe, Guta, and Taihe Districts in Jinzhou were randomly selected by stratified sampling method, who were given questionnaires and physical examinations.Results The incidence of senile hypertension in these communities averaged 40.3%(41.1% among males and 39.3% among females).incidence The difference was not statistically significant (P>0.05). A high incidence of hypertension was found among elders who had had very little schooling (44.3%), who were widowed (61.3%), and those who drank alcohol regularly (39.9%). Logistic regression analysis showed that regular alcohol consumption(OR= 1.659), overweight and central obesity(OR=3.110) were risk factors for hypertension among the elderly, while better education(OR= 0.600, 0.711) were protective factors. Body mass indexinteracted with the ratio of waist to hip.Conclusion Hypertension is prevalent in the elderly in Jinzhou , and is influenced by a number of factors. Community healthcare providers should take appropriate precautions to reduce the incident of hypertension in the elderly.

Objective To observe and explore the effectiveness of butylphthalide soft capsules (NBP) and nimodipine (NMD) in patients with vascular dementia (VD) after stroke.Methods A total of 96 patients with VD in General Hospital of Guangzhou Military Command were selected between May 2012 and August 2015. They were randomized into two groups of 48 patients: the NBP group and NMD group. The NBP group was treated orally with NBP while the NMD group was treated orally with NMD for 6 months respectively. The efficacy was evaluated by the cognitive function scale (MMSE, MOCA) and daily life ability scale (ADL score) before treatment and at 3 months and 6 months after treatment.The incidence of adverse reactions in both groups was recorded.Results The difference in MMSE, MOCA and ADL scores before treatment between the two groups was not statistically significant (P>0.05), but MMSE and MOCA scores in the NBP group were significantly higher than those of the NMD group 3 months and 6 months after treatment, , while ADL score was significantly lower than that of the NMD group (P＜0.01). The adverse reaction rate was 10.4% (5/48) and 22.9% (11/48) respectively in the NBP group, and 22.9% (11/48) in the NMD group. The difference was statistically significant (P＜0.05).Conclusion Both NBP and NMD treatment can significantly improve the cognitive function and daily life ability of VD patients, and the curative effect is remarkable, but NBP is more effective and safer than NMD.

Objective To investigate the mastery HIV/AIDS-related knowledge, attitude and behavior among autumn recruits in a certain troop so as to contribute to implementation of AIDS-related health education and prevention of HIV/AIDS.Methods A cross-sectional study was conducted with a standardized questionnaire and 118 recruits were enrolled. Factors which influenced the awareness rate of AIDS-related knowledge and attitude were analyzed.Results The awareness rate of AIDS-related knowledge was 85%, but the awareness rate of non-transmission routes was very low. For example, only 38.1% of these recruits knew that mosquito bite could not transmit HIV. There was difference in the awareness of AIDS-related knowledge between recruits with different demographic characteristics. The awareness rate of AIDS-related knowledge among recruits with a education below high school was significantly lower than that among recruits with a high school and university education.Conclusion The awareness rate of AIDS-related knowledge among recruits is very high, but the awareness rate of non-transmission routes of AIDS is very low . It is necessary to improve education related to prevention of AIDS and to cultivate a healthy lifestyle and behavior away from HIV/ AIDS.

Objective To observe the effect and influence factors of TCM health care intervention on patients with diabetic foot so as to contribute to the prognosis of diabetic foot.Methods 100 One hundred cases of hospitalized patients with diabetic foot between Oct 2012 and Oct 2014 with diabetic foot were randomly divided into control group (no TCM intervention) and intervention group (with TCM intervention). The recovery, cognitive and self-nursing ability were observed, while influence factors were analyzed. Results The scores of recovery, cognitive and self-nursing ability of patients in intervention group were not only higher than those of control group, but increased over time (P<0.05). Survival analysis showed that patients of intervention group took much less time to achieve a good effect than control group (P<0.05).Multiple factors analysis showed that levels of education and race were independent factors that influenced the effect of intervention.Conclusion TCM intervention can contribute to the rehabilitation of patients with diabetic foot.

Objective To evaluate the relationships between the stability of carotid atherosclerotic plaques and clinical symptoms of carotid atherosclerosis using contrast-enhanced ultrasonography.Methods Fifty-six patients with carotid atherosclerotic plaques were examined with contrast-enhanced ultrasonography. The contrast agent visualization of the carotid atherosclerotic plaques was analyzed and compared with their clinical symptoms.Results Twenty-six patients who had suffered from obvious clinical symptoms were entirely visualized. Among the thirty patients who had no apparent clinical symptoms, 17 were sparsely visualized, and there was no visualization in the other 13 patients.Conclusion Contrast-enhanced ultrasonography can observe microcirculation in carotid atherosclerotic plaques in real-time, and assess the stability of carotid atherosclerotic plaques.

Objective To investigate and analyze the relationships between the expression of uPA and PAI-1 and breast cancer and its prognosis.Methods Between March 2012 and May 2014, 82 cases of breast cancer and 42 cases of benign breast tumor were selected in our hospital. The expression levels of uPA and PAI-1 were detected in both groups of patients, and the correlation between the expression level and breast cancer was analyzed. The cases of breast cancer included 27 cases of recurrence, 55 cases without recurrence, or alternatively 24 cases without metastasis, 58 cases without metastasis. The relationships between the positive expression rate of uPA and PAI-1 and prognosis of the patients after treatment was analyzed.Results The positive expression rate of uPA and PAI-1 in breast cancer group was higher than that in benign breast tumor group (P＜0.05). The positive expression rate of PAI-1 and uPA in breast cancers with recurrence or metastasis was higher than in those without recurrence or metastasis(P＜0.05). Logistic regression analysis showed that OR values of uPA and PAI-1 in breast cancer, breast cancer with recurrence or metastasis were above zero.Conclusion Expressions of PAI-1 and uPA are correlated with breast cancer and its prognosis.

Objective To compare the diagnostic accuracy of the serum CA125 level combined with transvaginal color Doppler ultrasound and contrast-enhanced ultrasound respectively in ovarian tumors.Methods A total of 200 patients with suspected ovarian cancer treated in our hospital between Jan 2012 and Mar 2015 were examined with biopsy guided by transvaginal color Doppler ultrasound and contrast-enhanced ultrasound while their serum CA125 levels were detected. 182 cases were eventually confirmed to be ovarian tumors by pathological examinations of tumor tissues. The difference in diagnostic accuracy was compared between the above four examination methods.Results The diagnostic sensitivity, specificity, positive likelihood ratio and diagnostic accuracy of CA125 combined with biopsy guided by transvaginal color Doppler and contrast-enhanced ultrasound were higher than those of single transvaginal color Doppler ultrasound or contrast-enhanced ultrasound. The diagnostic Results of serum CA125 combined with contrast-enhanced ultrasound guided biopsy were significantly better than those of serum CA125 combined with transvaginal color Doppler ultrasound examination(P<0.05).Conclusion CA125 level combined with biopsy guided by contrast-enhanced ultrasound can improve the diagnostic accuracy of ovarian tumors.

Objective To compare the disinfection effects between manual cleaning and automatic cleaning and disinfection machines for digestive endoscopy. Methods One hundred digestive endoscopes which had been used between August 2014.8 and March 2015 were randomly divided into the experimental group and the control group (each n=50). The endoscopes in the experimental group were cleaned and disinfected by automatic washer disinfectors and those in the control group by hand.Results There was no difference in the total effective rate, which was 100% in both groups. Conclusion Although no difference is observed in the total effective rate between the two cleaning methods, automatic washer disinfectors can save manpower and time.They should be used preferably in clinical practice.

Objective To explore the effect of the planning, implementation, inspection, processing (PDCA) cycle method on the recovery of hospital medical records .Methods The basic causes of overdue recovery of medical records were retrospectively analyzed at a Center for Medical Records Statistics of a hospital from 2011 to 2013. The PDCA cycle method of management was used for the recovery of medical records in 2014. Medical records and the recovery effect in 2014 were compared with those of the previous three years to evaluate the PDCA cycle method.Results The delayed recovery of medical records between 2011 and 2013 was mainly due to delays in the examination reports (19.9%), those in pathological reports d (19.0%), and multiple department transference therapies (17.1 %).The rate of delayed recovery of medical records was 6.0% in 2014, significantly lower than in 2011, 2012 or 2013 (19.0%, 18.0% and 20.0%, respectively).ConclusionThePDCA cycle method can significantly improve the rate at which hospital medical records are recovered in three days after patients are discharged.

Objective To investigate the current status of HIV prescreening laboratories in east part of Lanzhou Military Command in order to promote accuracy, biological safety and standardized quality management in HIV detection.Methods Field investigation and blind samples examination were used to evaluate the working conditions of prescreening labs.Results All of the twelve labs had the ability for HIV prescreening test. The process of detection was consistent with the requirement of HIV prescreening and Results of 10 samples were all correct. However, some labs also had some problems in hardware facilities, software facilities and quality management.Conclusion The HIV prescreening labs in the east part of Lanzhou military command can carry out HIV prescreening detection service. But there were some difference in quality control between labs and should take some measurements to solve the problems.

Objective To explore the main factors which influenced sanitation safety in self-made food in order to provide reference for the investigation and prevention of similar incidents.Methods Retrospective investigation was carried out and the samples of self-made medicinal wine were collected and tested on the spot and in laboratory, and the process of self-made medicinal wine was imitated to find out the main causes of acroisa in this incident.ResultsAccording to the Results of rapid test on the spot and laboratory analysis, the methanol contents of both kinds of self-made medicinal wines in the accident exceeded the standard (≤0.04g/100ml) seriously and the highest value of laboratory detection reached 1.235g/100ml. Methanol detection Results of laboratory processing imitation examination showed that the methanol contents were 0.098g/100ml and 0.067g /100ml in the two self-made medicinal wines respectively after soaking 60 days. Although methanol contents changed along with the soaking time, methanol contents were beyond the standard and the value reached 0.135g /100ml after 300 days in 1# medicinal wine which was a significant difference compared with that of accident spot (1.235 g/100ml).Conclusion Two kinds of self-made medicinal wines with the formulas provided by the restaurant manager could produce methanol which were beyond the national standard after soaking 30 days and the methanol content increased over time. Therefore, the excessive methanol in self-made medicinal wine was the major cause of acroisa. To ensure the safety, medicinal wine must be made in accordance with standard process under professional guidance.

Objective To investigate the association between BRAF mutation and colon cancer with stomach cancer, and between BRAF mutation and gene expression of PRDX6 and PRDX1, and to analyze the role of PRDX1 and PRDX6 gene in colon cancer with other primary cancers.Methods A total of 47 patients with colon cancer complicated by gastric cancer samples were collected. Mutation of BRAF and MLH1 genes were detected by PNAClampTM kit, and chi-square test was used to compare the relationship between mutations and related characteristics. Expressions of PRDX1 and PRDX6 were quantified by qPCR and Western blot in samples with and without BRAF mutation. The plasmid with the BRAF mutation was transferred into human HCT-116 colon cancer cells to investigate the expression levels of PRDX1 and PRDX6.Results BRAF mutation had no correlation with age, cancer location, multiple cancer time intervals and the number of cancer or family history of cancer; while MLH1 mutation had correlation with cancer multiple time intervals and the number of cancer and family cancer history (P<0.05). PRDX1 and PRDX6 expression levels were correlated with the degree of the BRAF mutation and the number of primary cancer in colon cancer (P<0.05). PRDX1 and PRDX6 might be the target of BRAF mutation inhibition.Conclusion BRAF mutation rate in colon cancer complicated by gastric cancer is relatively low in the Chinese population. When using BRAF mutation as a marker for detection, other genes such as MLH1 gene mutation may be as auxiliary. BRAF mutation inhibits PRDX1 and PRDX6, which in turn may affect the number of primary cancer.

Objective To establish a method for determination of Arsenic (As) in foods.Methods The foods were dissolved by microwave and Arsenic in them was determined by double-channel hydride generation-atomic fluorescence spectrometry.Results The arsenic content was linear with the fluorescence intensity in the range of 0μg/L～10μg/L with correlation coefficient of 0.9994.The detection limit of this method was 0.006mg/kg.Recovery experiment was carried out and recovery was 96.1%～104.9%.Conclusion This method is accurate,rapid and convenient.

Objective To understand the mental health status of the military recruits, so as to provide the basis for recruits screening, prevention of psychological problems and corresponding mental health guidance.Methods Figure Searching Test (FST), Cattell Sixteen Personality Factor Questionnaire(16PF), Symptom Checklist(SCL-90) and Minnesota Multiphasic Personality Inventory(MMPI) were used to make psychological tests for 1602 ordinary new recruits in 2014.Results (1) The overall scores of FST test presented a skewed distribution, the scores ranged from 4 to 6.99 accounted for 48.8%. (2) Compared with military norm, the scores of sensitivity, vigilance, fantasy, anxiety, tension, cowardice and bold, creative personality factor of 16 PF tests were lower than the norm, while the other factors scores increased (P<0.01). (3) All the factor scores of SCL-90 test were lower than the national norm and recruits norm score in China (P<0.01). (4) Three recruits had potential psychological problems according to the Results of first three tests and opinions of their companies. A total of 46 recruits were screened with psychological problems by MMPI test among 154 recruits, then they had interviews with experts. (5) Two months later, 45 people (one was sent back home) were tested with SCL-90 test for the second time, the scores of all the factors and the total were statistically less than the national norm and Chinese recruits norm (P < 0.01), only one of them the scores of forced, depression and paranoid were more than 3.Conclusion In general, mental health of military recruits in 2014 are fairly good. FST, 16PF and SCL - 90 as an early screening, MMPI as the secondary screening, can comprehensively and systematically reflect the psychological status of recruits.

Objective This article aims to probe the effects of the field comprehensive training on military academy freshmen’s mental health, mental quality and emotions, which may provide evidences for the field comprehensive training scheme.Methods The participants were divided into the experimental group (316persons) and the control group (198persons). After accepting basic training on campus, the experimental group proceeded to a distance of 330km’s field comprehensive training while the control group has been training on campus. Methods Symptom Checklist 90(SCL-90), Trait Anxiety Inventory(TAI), Self-Rating Depression Scale(SDS) and Self-Rating Anxiety Scale(SAS) were adopted to conduct psychological evaluation for the two groups.Results①After training, the experimental group SCL - 90 every factor score significantly reduced (P<0.05, P<0.01); the Control group SCL – 90 anxiety factor score was significantly lower (P<0.01). ②?After training, trait anxiety score for the experimental group was significantly decreased (P<0.01); the control group mood every factor score had no significant difference (P>0.05).Conclusion Field comprehensive training is an effective method for improving military academy cadets’ mental health and reliving their anxiety as well as depression.

Objective To investigate the changes of fasting blood-glucose and vital signs of submarine crew during sea voyage.Methods Fasting blood-glucose, breath, pulse and mean arterial pressure(MAP) of fifty submariner who were selected as subjects were detected before, the early, middle, late and after sea voyage.Results Compared with that before sailing, fasting blood-glucose in the early, middle, late stage of the sea voyage(P<0.01) and after sea voyage(P<0.05) were elevated significantly. Compared with that before sea voyage, breath and MAP in the early, late stage of the sea voyage were increased markedly (P<0.05).Conclusion with that before and after sea voyage, pulse in the early stage of the sea voyage were increased significantly (P<0.05). Conclusions The present study has revealed that fasting blood-glucose, breath, pulse and MAP of conventional submarine crew can get significantly improved during navigation, especially in the early stage of the sea voyage.

Objective to research and analysis the freshman military training during the anxiety status and its influencing factors, to provide basis for early intervention.Methods using ZUNG self rating anxiety scale (SAS) of some colleges and universities of the new entrance to investigate 2158 people take part in military training. Grouped according to whether anxiety, the anxiety of factors affecting coke single factor analysis and multivariable Logistic regression model analysis.Results 1574 people anxiety, the incidence of anxiety was 72.94%; Single factor analysis Results show that the anxiety group of students, as an only child, is poor, for the city is the proportion of single-parent families is higher than the healthy group (P<0.01);Anxiety group's strong adaptability to the drillmaster's support, in the process of their military training and guidance, in the process of their military training instructor interaction ratio is lower than the healthy group (P<0.01). Multiple factors Logistic regression model analysis Results show that in the balance of the two groups of students, is the only child is, is poor, single-parent families and strong adaptability after military training during the support from the instructor to guide (OR=7.139, 95% CI= 5.540~9.712) during military training, and interact with the instructor (OR= 4.416, 95% CI=2.571~7.518) is statistically significant.Conclusion during military training in college freshmen by the drillmaster's concern, support and guidance, and more interaction with the instructor can reduce the incidence of anxiety.

Objective To determine the effects of physical exercises before recruitment on the level of anxiety emotions during recruit training and to investigate whether the physical exercises before recruitment would help to reduce the anxiety level of the recruits during their training.MethodsThe self-designed Preinduction Physical Activity Level (PPAI) questionnaire and the State-Trait Anxiety Inventory (STAI) questionnaire were finished one week after the recruits was arrived.ResultsThe anxiety level of the recruits, who taking physical exercises that no less than 30 minutes for at least 3 times a week, and lasted over 3 months, was significantly lower than those taking less physical exercises (P<0.01). Middle-distance running and basketball reduced the level of both state anxiety and trait anxiety (P<0.01). Table tennis and walking could significantly reduced the level of trait anxiety (P<0.05). The level of state anxiety and trait anxiety was significantly higher in the recruits who worried about training injuries than those who didn't worry about training injuries (P<0.01). There was no statistical significance in the level of state anxiety and trait anxiety of recruits with the history of sports injury or no (P>0.05).ConclusionA good habit of taking physical exercises before recruitment would help to reduce the level of state and trait anxiety during recruit training. We suggested that those military service-age individuals should take long term and regular exercises which would help the recruits to adapt to army life as soon as possible and impove the fighting power.

Objective Study tirofiban combined with nifedipine controlled release tablets, QuMei Trimetazidine clinical curative effect of trimetazidine on patients with refractory angina pectorisMethods The patients with refractory angina were divided into control group and observation group. The control group was given routine symptomatic treatment, patients in the observation group were treated with tirofiban combined with nifedipine controlled release tablets, QuMei Trimetazidine. Compare the difference of curative effect between the two groups of patients.Results The effect of the observation group was better than that of the control group(P＜0.05). There was no significant difference in serum OX40L, sICAM-1 and hs-CRP levels in the two groups before treatment (P>0.05). The serum levels of OX40L, sICAM-1 and hs-CRP in the observation group were significantly lower than those in the control group after treatment(P<0.05). There was no significant difference in the scores of angina pectoris in the two groups before treatment (P>0.05). After treatment, the observation group of patients with angina pectoris symptom score was significantly better than the control group (P<0.05).Conclusion Tirofiban combined with nifedipine controlled release tablets, QuMei trimetazidine in the treatment of refractory angina pectoris can effectively inhibit the formation of thrombus, and improve the clinical symptoms. Curative effect is distinct, and it is worth clinical promotion.

Acute lung injury / acute respiratory distress syndrome (ALI/ARDS) is a result of intravascular fluid penetration into the pulmonary interstitial and alveolar, causing a large amount of high permeability pulmonary edema. Pulmonary edema formation is an important pathological and pathophysiological change in the ALI/ARDS. In this paper, researches on pathogenesis of pulmonary edema induced by ALI/ARDS were reviewed according to the recent progress in pathophysiology, involving the function disorders of effector cells, the changes of the coagulation fibrinolysis system and the mechanisms of inflammatory reaction. This paper is expected to provide reference for further research.

Long-term environmental hazard exposure can result in varying degrees of impact on the development of the central nervous system(CNS) and on other functions, such as learning and memory, emotion and behavior. Corticotropin- releasing factor (CRF) is an endogenous neuropeptide found in recent years, which plays an important role in regulating the CNS need by binding to its G- protein receptors. This paper aims to review the effects of CRF and its receptor system on CNS injuries induced by environmental hazards.

Objective To explore new measures and mechanisms of modern medical services in schools affiliated to special branches of the armed forces in China by analyzing informatization, pluralization and developments associated with future wars. Modern medical services should be based on the ability to deal with war injuries, to prevent and treat training-caused illnesses, to adapt to the environment and to integrate medical services. Schools affiliated to special branches of the armed forces should keep pace with modern medical services, change the staff's ideas about medical services, and improve the mode and Methods of medical services to ensure medical service capability.

Water quality monitoring and improvement determine the healthy body of officers and soldiers in the field. Medical sergeants are responsible for water quality monitoring and improvement. How to train the skill of medical sergeants,Our practical teaching included the training content, the training model about water quality monitoring and improvement, so as to provide the basis for high-performance and scientific training this skill.