有氧运动对2型糖尿病大鼠骨骼肌ERK1/2活性的影响

doi:10.12047/j.cjap.5448.2017.008

中国应用生理学杂志 ›› 2017, Vol. 33 ›› Issue (1): 33-37.doi: 10.12047/j.cjap.5448.2017.008

有氧运动对2型糖尿病大鼠骨骼肌ERK1/2活性的影响

李艳辉, 衣雪洁

沈阳体育学院运动人体科学学院, 辽宁沈阳 110102

收稿日期:2015-09-06 修回日期:2016-06-17 出版日期:2017-01-28 发布日期:2018-06-19
通讯作者: 衣雪洁,Tel:024-89166365,E-mail:yixuejie8387@163.com E-mail:yixuejie8387@163.com
基金资助:
辽宁省优秀人才支持计划项目（WR2013015）；沈阳体育学院重点学科建设项目（XKFX1511）

The effects of aerobic exercise on ERK1/2 activity in skeletal muscle of type 2 diabetic rats

LI Yan-hui, YI Xue-jie

School of Sports Science, Shenyang Sport University, Shenyang 110102, China

Received:2015-09-06 Revised:2016-06-17 Online:2017-01-28 Published:2018-06-19
Supported by:
辽宁省优秀人才支持计划项目（WR2013015）；沈阳体育学院重点学科建设项目（XKFX1511）

摘要/Abstract

摘要： 目的：观察有氧运动对2型糖尿病大鼠骨骼肌细胞外信号调节激酶（ERK1/2）活性的影响，探讨有氧运动对2型糖尿病的预防和调控机制。方法：将75只SD大鼠随机分为正常对照组（CON）、糖尿病对照1组（DC1）、糖尿病运动1组（DE1）、糖尿病对照2组（DC2）、糖尿病运动2组（DE2）5组（n=15）。正常对照组用普通饲料喂养，糖尿病组用高脂高糖配方饲料喂养。经过8周高脂高糖喂养后，糖尿病2组大鼠腹腔内注射链脲佐菌素（STZ），诱发2型糖尿病；糖尿病运动1组游泳的最后1周初和糖尿病对照1组同时注射STZ，注射剂量为35 mg/kg，3 d后尾部取血测血糖≥ 16.7 mmol/L为造模成功。运动干预8周后，测定大鼠血清胰岛素、骨骼肌中ERK1/2蛋白表达等指标。结果：①与正常对照组比较，糖尿病各对照组血液中总胆固醇（TC）、甘油三酯（TG）、低密度脂蛋白（LDL-C）、游离脂肪酸（FFA）显著升高（P<0.05，P<0.01），空腹血糖（FBG）、胰岛素（FIN）含量和胰岛素抵抗指数（HOMA-IR）显著升高（P<0.01），ERK1/2磷酸化的蛋白表达显著下降（P<0.05），糖尿病对照2组ERK1/2蛋白含量显著下降（P<0.05）；②8周游泳运动后，与糖尿病对照组比较，糖尿病运动组血液中TC、TG、FFA、LDL-C显著下降（P<0.05），FBG、FIN、HOMA-IR显著下降（P<0.05，P<0.01），ERK1/2磷酸化蛋白表达显著升高（P<0.05）。结论：长时间有氧运动，增加了骨骼肌ERK1/2磷酸化水平，改善了2型糖尿病大鼠胰岛素抵抗的状况，降低血糖。这可能是改善糖代谢紊乱，提高胰岛素敏感性的机制之一。

关键词: 有氧运动, 胰岛素抵抗, 2型糖尿病, ERK1/2

Abstract: Objective: To observe the effects of aerobic exercise on activity of extracellular signal-regulated kinase (ERK1/2) in skeletal muscle of type 2 diabetic rats and explore the prevention and control mechanism of aerobic exercise on type 2 diabetes.Methods: Seventy five SD rats were randomly divided into:normal control group(CON) including 15 rats was fed with normal diet, diabetes control group 1(DC1), diabetes exercise group 1(DE1), diabetes control group 2(DC2), diabetes exercise group 2(DE2). Diabetes model group were fed with high-fatty and high-sugar diet. The diabetes model rats were fed with high-fatty and high-sugar diet for 8 weeks,.Diabetes group 2 rats were injected intraperitoneal streptozotocin(STZ)to induce type 2 diabetes. At the early stage of last swimming week, diabetes exercise group1 and diabetes control group 1 were injected with STZ (35 mg/kg) at the same time, After three days, if the level of blood glucose was ≥ 16.7mmol/L, the model was successful. After 8 week-interventions, all the rats were killed, the serum levels of insulin and the expression of ERK1/2 protein in skeletal muscle were determined.Results: ①Compared with the normal control group, the levels of total cholesterol(TC), triglyceride(TG), low density lipoprotein cholesterin(LDL-C), free fatty acid(FFA), fasting blood-glucose(FBG), fasting insulin(FIN) and insulin resistance index(HOMA-IR) were increased significantly in diabetes control group(P<0.05 or 0.01). However, the expression of ERK1/2 phosphorylation protein was decreased obviously in diabetes control group. The content of ERK1/2 protein was decreased obviously in diabetes control group 2 had (P<0.05). 2.After eight weeks' swimming, compared with the diabetes control group, the levels of TC,TG, FFA, LDL-C, FBG, FIN and HOMA-IR were decreased significantly in diabetes exercise group(P<0.05 or 0.01). At the same time, the expression of ERK1/2 phosphorylation protein was increased obviously in diabetes exercise group(P<0.05).Conclusion: Long-term aerobic exercise can improve the skeletal muscle ERK1/2 phosphorylation and insulin resistance of type 2 diabetic rats, thereby lowering blood glucose. It is probably one of the mechanisms to improve glucose metabolism disorders and insulin sensitivity.

Key words: aerobic exercise, insulin resistance, type 2 diabetes, ERK1/2

李艳辉, 衣雪洁. 有氧运动对2型糖尿病大鼠骨骼肌ERK1/2活性的影响[J]. 中国应用生理学杂志, 2017, 33(1): 33-37.

LI Yan-hui, YI Xue-jie. The effects of aerobic exercise on ERK1/2 activity in skeletal muscle of type 2 diabetic rats[J]. CJAP, 2017, 33(1): 33-37.

参考文献

[1] 李锐. Ⅱ型糖尿病患者运动疗法的研究进展[J]. 哈尔滨体育学院学报, 2014, 32(6):92-96.
[2] 周静, 高晟, 江立新, 等. 中药及其有效成分治疗胰岛素抵抗的分子机制研究进展[J]. 天津药学, 2015, 27(3):57-61.
[3] Cartee GD. Mechanisms for greater insulin-stimulated glucose uptake in normal and insulin-resistant skeletal muscle after a-cute exercise[J]. Am J Physiol Endocrinol Metab, 2015, 309(12):E949-959.
[4] Cusi K, Maezono K, Osman A, et al. Insulin resistance dif-fe rentially affects the PI3-Kinase and MAP Kinase-mediated signaling in human muscle[J]. J Clin Invest, 2000, 105(3):311-320.
[5] 吴军发, 吴毅, 胡永善, 等. 运动改善原发2型糖尿病大鼠血糖和血脂代谢紊乱的实验研究[J]. 中华物理医学与康复杂志, 2002, 24(9):536-538.
[6] 马泽兵, 胡晨, 罗强, 等. 胰岛素抵抗及其运动干预的研究进展[J]. 辽宁体育科技, 2014, 36(3):44-47.
[7] Kyriakis JM, Avruch J. Mam malian mitogen-activated pro-tein kinases signal transduction pathways activated by stress and inflammation[J]. Physiol Rev, 2001, 81(2):807-869.
[8] Ren ZQ, Zhang PB, Zhang XZ, et al. Duodenal-jejunal ex-clusion improves insulin resistance in type 2 diabetic rats by upregulating the hepatic insulin signaling pathway[J]. Nutri-tion, 2015, 31(5):733-739.
[9] 马连环, 刘建. ERK1/2的研究进展[J]. 国外医学(生理、病理科学与临床分册), 2005, 25(3):279-282.
[10] Sakamoto K, Goodyear LJ. Invited review:Intracellular sig-naling in contracting skeletal muscle[J]. J Appl Physiol, 2002, 93(1):369-383.
[11] Gorin Y, Ricono JM, Wagner B, et al. Angiotensin -in-duced ERK1/2 activation and protein synthesis are redox-de-pendent in glomerular mesangial cells[J]. Biochem J, 2004, 381(Pt1):231-239.
[12] 吴毅. 运动疗法改善糖尿病大鼠糖代谢异常作用机制的实验研究[D]. 上海, 复旦大学:2007.
[13] Chen R, Li X, Lu S, et al. Role of extracelluar signal-regu-lated kinases 1 and 2 p38 mitogen-activated protein kinase pathways in regulating replication of Peniclilium marneffein in human macrophages[J]. Microbes Inf ect, 2014, 16(5):401-408.
[14] 王小娟, 李建华, 边仁秀. 有氧运动对2型糖尿病大鼠腓肠肌氧化应激及MAPKs信号通路的影响[J]. 中国运动医学杂志, 2012, 31(9):800-805.
[15] Goodyear LJ, Chang PY, Sherwood DJ, et al. Effects of ex-ercise and insulin on mitogen-activated protein kinase signal-ing pathways in rat skeletal muscle[J]. Am J Physiol, 1996, 271(2Pt1):E403-408.
[16] 曹师承. 运动对大鼠骨骼肌PI3K/AKt/mTor与MAPK 信号转导途径的影响[D]. 沈阳, 中国医科大学:2007.

有氧运动对2型糖尿病大鼠骨骼肌ERK1/2活性的影响

The effects of aerobic exercise on ERK1/2 activity in skeletal muscle of type 2 diabetic rats

PDF (PC)

可视化

摘要/Abstract

引用本文

使用本文

参考文献

相关文章 15

编辑推荐

Metrics

本文评价

[1]	孙晓娟, 冯武龙, 侯娜, 李娜, 姜蔚丽. 有氧运动和白藜芦醇对2型糖尿病大鼠肾脏JAK2及TGF-β1的影响[J]. 中国应用生理学杂志, 2020, 36(3): 202-206.
[2]	完建永, 张勇, 刘世强, 华田苗, 孙庆艳. 运动对胰岛素抵抗大鼠肝脏BIM信号通路的影响^*[J]. 中国应用生理学杂志, 2020, 36(2): 115-118.
[3]	衣雪洁, 孙玉霞, 姚婷婷, 李晶, 高畅, 刘璐, 曹师承, 常波, 张翠萍. 急、慢性运动对2型糖尿病大鼠脂肪PI3K/AKT/GLUT4通路的影响[J]. 中国应用生理学杂志, 2020, 36(1): 12-16.
[4]	卢漫漫, 陈家耀, 钮鸣宇, 吴卫东. 有氧运动对高脂饮食小鼠肝脏中CLK2蛋白表达的影响[J]. 中国应用生理学杂志, 2020, 36(1): 23-26.
[5]	白淑芝, 徐娜, 王跃虹, 李鸿珠, 李宏霞. 糖尿病大鼠心肌功能的改变及其机制^*[J]. 中国应用生理学杂志, 2019, 35(5): 390-392.
[6]	马涛, 高炳宏, 李涛. 8周有氧水上划艇训练对赛艇运动员某些儿茶酚胺和心率变异性指标的影响^*[J]. 中国应用生理学杂志, 2019, 35(3): 219-222.
[7]	项伟玲, 金丽琴, 高峰, 肖敏, 陈燕. 黑苦荞茎叶对2型糖尿病小鼠的治疗作用及其对其胰腺、脾脏的影响^*[J]. 中国应用生理学杂志, 2019, 35(2): 140-144.
[8]	王小梅, 景会锋. 有氧运动和谷氨酰胺对二型糖尿病大鼠氧化应激及相关因子表达的影响^*[J]. 中国应用生理学杂志, 2019, 35(2): 150-154.
[9]	马涛, 高炳宏, 李涛. 8周有氧水上划艇训练对赛艇运动员某些血液生化指标的影响[J]. 中国应用生理学杂志, 2019, 35(1): 38-40.
[10]	谢克俭, 黄玲, 屈小虎, 李雪, 王少杰, 肖敏. 口服AdipoRon对2型糖尿病小鼠脾脏和胰腺功能的影响[J]. 中国应用生理学杂志, 2019, 35(1): 60-64.
[11]	黄玲, 屈小虎, 陈慧, 谢克俭, 肖敏. AdipoRon对2型糖尿病小鼠肾脏损伤的干预作用[J]. 中国应用生理学杂志, 2018, 34(6): 568-571.
[12]	张静, 缪刚, 曹建民, 周海涛, 牛衍龙, 张岳, 任奕, 包欣悦, 邢雅雯. 有氧运动联合破壁蛋白核小球藻对高脂膳食大鼠脂代谢某些指标的影响[J]. 中国应用生理学杂志, 2018, 34(5): 445-449.
[13]	彭勇, 史绍蓉, 黄思敏, 郭远盘, 徐哲, 万莉莉, 雷雄. 4周中等强度有氧运动诱导大鼠心房肌蛋白质组差异表达的研究[J]. 中国应用生理学杂志, 2018, 34(5): 450-456.
[14]	朱雅冰, 贾改丽, 陆嘉辉, 张茂表, 李军, 曹红. 脊髓自噬功能激活与大鼠2型糖尿病神经病理性疼痛的关系[J]. 中国应用生理学杂志, 2018, 34(4): 318-323.
[15]	苏敏, 柏友萍, 宋威巍, 王明, 沈瑞睿, 杜康健, 夏行权, 聂刘旺. 运动对老年肥胖大鼠脂联素的影响[J]. 中国应用生理学杂志, 2018, 34(4): 345-349.