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中国应用生理学杂志 ›› 2018, Vol. 34 ›› Issue (2): 111-114.doi: 10.12047/j.cjap.5541.2018.027

• 研究论文 • 上一篇    下一篇

APP/PS1双转基因小鼠的繁育、鉴定及评价

谭龙1,2, 李海强1,4, 李宜波1,3, 刘伟1, 庞伟1, 蒋与刚1   

  1. 1. 军事科学院军事医学研究院环境医学与作业医学研究所, 天津 300050;
    2. 天津医科大学公共卫生学院, 天津 300070;
    3. 天津科技大学食品工程与生物技术学院, 天津 300457;
    4. 烟台高新技术开发区医院, 烟台 264006
  • 收稿日期:2016-12-20 修回日期:2017-12-02 出版日期:2018-03-28 发布日期:2018-05-22
  • 通讯作者: 蒋与刚 E-mail:jyg1967@126.com
  • 基金资助:
    天津市应用基础与前沿技术研究计划重点项目(14JCZDJC36100)

Reproduction,genotype identification and evaluation of APP/PS1 transgenic mice

TAN Long1,2, LI Hai-qiang1,4, LI Yi-bo1,3, LIU Wei1, PANG Wei1, JIANG Yu-gang1   

  1. 1. Institute of Environmental and Operational Medicine, Military Medical Research Institute of Military Academy, Tianjin 300050;
    2. School of Public Health, Tianjin Medical University, Tianjin 300070;
    3. College of Food Engineering and Biotechnology, Tianjin University of Science and Technology, Tianjin 300457;
    4. Yantai Economic and Technological Development Area Hospital, Yantai 264006, China
  • Received:2016-12-20 Revised:2017-12-02 Online:2018-03-28 Published:2018-05-22
  • Supported by:
    天津市应用基础与前沿技术研究计划重点项目(14JCZDJC36100)

摘要: 目的:评价APP/PS1双转基因小鼠基因表达及认知行为能力的变化,为AD的相关研究提供有效的动物模型。方法:采用雄、雌鼠1:1合笼配对的方式,令APP/PS1双转基因小鼠自然交配进行繁育。PCR鉴定APP/PS1双转基因鼠仔鼠的基因型后,选择APP/PS1阳性小鼠作为模型(AD)组,同批APP/PS1阴性为对照(CT)组,每组8只小鼠。以Morris水迷宫实验检测仔鼠的空间学习记忆能力,以HE染色、刚果红染色观察仔鼠脑片组织病理学改变。结果:①APP/PS1双转基因鼠仔鼠基因经PCR扩增,出现约360 bp的目的基因条带,表明成功繁育出转入APP/PS1基因的仔鼠;②Morris水迷宫实验结果显示,与7月龄阴性小鼠(CT组)比较,同月龄的双转基因AD组小鼠的空间学习记忆能力明显降低(P<0.05);③HE染色结果显示,AD组小鼠海马结构及细胞形态出现明显异常;刚果红染色结果显示,AD组小鼠脑片组织出现β淀粉样蛋白斑块沉积。结论:APP/PS1双转基因小鼠较好地模拟了AD的病理变化及行为学特征,可作为研究AD发病机制及开发AD防治药物的实验工具。

关键词: 阿尔茨海默病, APP/PS1, 认知功能, 病理学

Abstract: Objective:To identify the genotype of (APP/PS1) transgenic mice and evaluate the changing of cognitive and behavioral fu nctions, provide an effective animal model for the Alzheimer's disease (AD) research. Methods:Male APP/PS1 transgenic mice mated with female APP/PS1 transgenic mice, and the genotype of their filial mice was identified by PCR. The APP +/PS1 + mice were assigned into AD model group (AD group, n=8), and the APP/PS1 mice were assigned into control group (CT group, n=8). The Morris water maze test was carried out to detect the capacity of learning and memory of mice. After that, the mice were sacrificed and the brain tissues were sampled and stained by HE and congo red for the pathological examination. Results:①A APP/PS1 genome DNA about 360 bp size was detected. The methods of feeding and breeding were successful to attain APP/PS1 transgenic mice.②Statistical significance was found in the differences of the capacity of learning and memory between 7-month-old APP/PS1 positive mice and negative mice (P<0.05).③The results of HE stain showed that the structure and cellular morphology of hippocampus of AD mice were obviously abnormal. The results of congo red stain showed that positive amyloid plaque was observed in brains of AD mice. Conclusion:APP/PS1 transgenic mice present typical symptoms and behaviors of Alzheimer's disease. The transgenic mouse is an effective tool for the research and prevention of AD.

Key words: Alzheimer's disease, APP/PS1, cognition, pathology

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