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中国应用生理学杂志 ›› 2016, Vol. 32 ›› Issue (6): 563-566.doi: 10.13459/j.cnki.cjap.2016.06.017

• 研究论文 • 上一篇    下一篇

当归黄芪提取物对慢性腹膜功能衰竭大鼠腹膜功能、结构及TGF-β1表达的影响

杜丽东1,2, 吴国泰1,2, 王凤玲3, 张瑛1, 任远1,2   

  1. 1. 甘肃中医药大学, 兰州 730000;
    2. 甘肃省中药药理与毒理学重点实验室, 兰州 730000;
    3. 甘肃省第二人民医院, 兰州 730000
  • 出版日期:2016-11-28 发布日期:2018-06-19
  • 通讯作者: 任远,Tel:0931-8762653;E-mail:Leyuan816@163.com E-mail:Leyuan816@163.com
  • 基金资助:
    甘肃省自然科学基金项目(1310RJZA090);甘肃中医学院中青年基金项目(BH2012-023)

Effects of extraction of Angelicae sinensis and Astragalus mongholicus on the peritoneal structure, functions and TGF-β1 expression in the chronic peritoneal failure rats

DU Li-dong1,2, WU Guo-tai1,2, WANG Feng-ling3, ZHANG Ying1, REN Yuan1,2   

  1. 1. Gansu University of Chinese Medicine, Lanzhou 730000, China;
    2. Gansu Province Key Laboratory of Pharmacology and Toxicology of Traditional Chinese Medicine, Lanzhou 730000, China;
    3. The Second People's Hospital of Gansu Province, Lanzhou 730000, China
  • Online:2016-11-28 Published:2018-06-19
  • Supported by:
    甘肃省自然科学基金项目(1310RJZA090);甘肃中医学院中青年基金项目(BH2012-023)

摘要: 目的:观察当归黄芪提取物(EAA)对大鼠腹膜功能、结构及转化生长因子β1(TGF-β1)表达的影响。方法:50只SD大鼠随机分为正常对照组、模型对照组、阳性对照组、EAA高、低剂量组(n=10)。除正常对照组外,其余4组大鼠每天腹腔注射4.25%高糖腹透液100 ml/kg,连续40 d,并在第8天、10天、12天腹腔注射大肠杆菌脂多糖(LPS)5 mg/kg,建立大鼠慢性腹膜功能衰竭模型,造模的同时灌胃给予相应药物。通过称大鼠体重和观察活动状态,测定超滤量、腹膜转运功能参数、壁层腹膜厚度、壁层腹膜TGF-β1的表达,研究EAA对大鼠腹膜功能、结构的保护作用及TGF-β1表达的影响。结果:EAA能升高高糖腹膜透析液联合LPS诱发的腹膜功能衰竭大鼠超滤量和腹膜转运功能参数;使大鼠腹膜增厚程度降低,TGF-β1表达减少。结论:EAA对大鼠腹膜功能、结构具有一定的保护作用且能抑制TGF-β1表达。

关键词: 当归黄芪提取物, 腹膜透析, 腹膜结构, 腹膜功能, TGF-β1, 大鼠

Abstract: Objective: To study the effects of extraction of Angelicae sinensis and Astragalus mongholicus (EAA) on the peritoneal structure, functions and transforming growth factor-β1(TGF-β1) expression in the chronic peritoneal failure rats. Methods: Fifty SD rats were randomly divided into normal control group, model control group, positive control group, high-dose and low-dose EAA group(n=10). The modeling rats were established by intraperitoneal injection(ip) 4.25% high-glucose peritoneal dialysate 100 ml/kg, lasted 40 d, and ip lipopolysaccharides (LPS) 5 mg/kg at 8thd, 10thd and 12thd. At the same time, the rats were treated with the corresponding drugs. The body weight and general states, the levels of ultrafiltration volume, the parameters of peritoneal transport function, the changes of peritoneal morphology and the TGF-β1 expression in parietal peritoneum of rats were observed. Results: EAA could increase the levels of ultrafiltration volume and improve the parameters of peritoneal transport function. The peritoneal thickness were decreased, and the TGF-β1 expression in parietal peritoneum were also lowered significantly. Conclusion: EAA has some protective effects on the peritoneal structure and functions, and can inhibit TGF-β1 expression.

Key words: Angelicae sinensis and Astragalus mongholicus, peritoneal dialysis, peritoneal structure, peritoneal function, TGF-β1, rats

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