强噪声对豚鼠认知功能和血清某些生化指标的影响

doi:10.13459/j.cnki.cjap.2016.05.001

中国应用生理学杂志 ›› 2016, Vol. 32 ›› Issue (5): 385-389.doi: 10.13459/j.cnki.cjap.2016.05.001

• 研究论文 • 下一篇

强噪声对豚鼠认知功能和血清某些生化指标的影响

赵晓雨, 刘淑红, 赵彤, 朱玲玲, 范明, 赵永岐, 房涛

军事医学科学院基础医学研究所军事认知科学研究室, 北京 100850

收稿日期:2015-05-11 修回日期:2016-06-27 出版日期:2016-09-28 发布日期:2018-06-20
通讯作者: 房涛,Tel:010-66932309;E-mail:fangtao@nic.bmi.ac.cn E-mail:fangtao@nic.bmi.ac.cn
基金资助:
国家军口"863"项目资助（8092017A）；国家科技重大专项课题资助（2012ZX09J12201-005）

Effects of strong noise on cognitive function and some biochemical indexes in serum of guinea pigs

ZHAO Xiao-yu, LIU Shu-hong, ZHAO Tong, ZHU Ling-ling, FAN Ming, ZHAO Yong-qi, FANG Tao

Institute of Basic Medical Science, Research Center for Military Cognition and Mental Hygiene of PLA, Academy of Military Medical Sciences, Beijing 100850, China

Received:2015-05-11 Revised:2016-06-27 Online:2016-09-28 Published:2018-06-20
Supported by:
国家军口"863"项目资助（8092017A）；国家科技重大专项课题资助（2012ZX09J12201-005）

摘要/Abstract

摘要： 目的：探讨强噪声对豚鼠认知功能和血清应激激素、代谢酶、神经肽、脑红蛋白等的影响，筛选噪声性认知功能损伤的敏感指标。方法：将24只豚鼠进行5 d的认知功能训练，第6天随机分为2组（n=12）：噪声组和对照组。噪声组在120 dB噪声下连续暴露4h后，用Morris水迷宫测试豚鼠的认知能力；用Elisa试剂盒检测豚鼠血清神经肽Y（NPY）、神经肽P（NPP）、脑红蛋白（NGB）、C反应蛋白（CRP）、皮质醇（CORT）、促肾上腺皮质激素（ACTH）、促肾上腺皮质激素释放激素（CRH）、肾上腺素（E）、去甲肾上腺素（NE）、血管紧张素Ⅱ（AngⅡ）、醛固酮（ALD）、催产素（OT）、血管升压素（VAP）含量、谷草转氨酶（ALT）、谷丙转氨酶（AST）、乳酸脱氢酶（LDH）、肌酸激酶（CK）的活性；用回归分析评价各项敏感指标对认知功能的影响及程度。结果：Morris水迷宫测试显示，与对照组相比，噪声组豚鼠的平均逃避潜伏期明显延长，跨越平台次数和目标象限停留时间均明显降低（P<0.01）。Elisa检测发现，除AngⅡ外，噪声组豚鼠血清中上述指标均与对照组有明显的统计学差异（P<0.05，P<0.01），其中变化显著且对认知功能影响较大的指标依次为CORT、NPY、NE、NGB、NPP、CK。结论：强噪声急性暴露引起豚鼠认知功能显著下降，可能与外周血应激激素、代谢酶、神经肽、脑红蛋白等生化指标的异常有关，并初步筛选出了CORT、NPY、NE、NGB、NPP、CK等与噪声性认知功能损伤有关的敏感指标群。

关键词: 强噪声, 认知, 血清, 神经肽, 应激激素, 代谢酶, 豚鼠

Abstract: Objective: To explore the effect of strong noise on cognitive function and stress hormones, biochemical metabolism enzymes, neuropeptide etc in theserum of guinea pigs, and screen the sensitive bio-markers of noise-induced cognitive impairment.Methods: Twenty-four guinea pigs were randomly divided into the noise exposure group and the control group, after the white noise at 120 dB continuously exposed for 4 h. Using Morris water maze to detect the cognitive ability of guinea pigs, and using Elisa kits to detect the content of neuropeptide Y (NPY), neuropeptide P (NPP), neuroglobin (NGB), c-reactive protein (CRP), cortisol (CORT), adrenocorticotropic hormone (ACTH), corticotropin releasing hormone (CRH) and epinephrine (E), norepinephrine (NE), angiotensin Ⅱ (AngⅡ), aldosterone (ALD), oxytocin (OT), vasopressin (VAP), alanine aminotransferase (ALT), aspartate transaminase (AST), lactate dehydrogenase (LDH), and creatine kinase (CK) in the serum of guinea pig. Multiple regression analysis was used to evaluatethe sensitive indexes for the influence of cognitive function.Results: Morris water maze test indicated that the average escape latency of noise exposure group extended obviously, cross platform number and residence time in target quadrant were significantly reduced compared with control group(P<0.01). Elisa results showed that the content of the biochemical indexes above of noise exposure grouphad significant statistical difference except AngⅡ compared with control group(P<0.01), among them, the content of CORT, NPY, NE, NGB, NPP, CK changed obviously and contributed greatly to cognitive in turn.Conclusion: Strong noise at 120dB continuous exposure for 4 h caused a significant decline on cognitive function of guinea pigs, it might be related to the abnormal levels of the physiological and biochemical indexes in serum such as stress hormones, biochemical metabolism enzyme, neuropeptides, neuroglobin etc, and we preliminarily filtrated several sensitive index associated with noise-induced cognitive impairment, including CORT、NPY、NE、NGB、NPP and CK.

Key words: strong noise, cognitive, serum, neuropeptides, stress hormone, biochemical metabolism enzymes, guinea pigs

赵晓雨, 刘淑红, 赵彤, 朱玲玲, 范明, 赵永岐, 房涛. 强噪声对豚鼠认知功能和血清某些生化指标的影响[J]. 中国应用生理学杂志, 2016, 32(5): 385-389.

ZHAO Xiao-yu, LIU Shu-hong, ZHAO Tong, ZHU Ling-ling, FAN Ming, ZHAO Yong-qi, FANG Tao. Effects of strong noise on cognitive function and some biochemical indexes in serum of guinea pigs[J]. CJAP, 2016, 32(5): 385-389.

参考文献

[1] Basner M, Babisch W, Davis A, et al.Auditory and non-auditory effects of noise on health[J]. The Lancet, 2014, 383(9925):1325-1332.
[2] Tzivian L, Winkler A, Dlugaj M, et al.Effect of long-term outdoor air pollution and noise on cognitive and psychological functions in adults[J]. IntJ Hyg Environ Health, 2015, 218(1):1-11.
[3] Arnsten AFT. Stress signalling pathways that impair prefrontal cortex structure and function[J]. Nat Rev Neurosci, 2009, 10(6):410-422.
[4] Wright B, Peters E, Ettinger U, et al.Understanding noise stress-inducedcognitive impairment in healthy adults and its implications for schizophrenia[J]. Noise Health, 2014, 16(70):166.
[5] Zheng Y, Meng M, Zhao C, et al.Impact of environmental noise on growth and neuropsychological development of newborn rats[J]. Anat Rec Hoboken NJ2007, 2014, 297(5):949-954.
[6] Kim T-W, Shin M-S, Park J-K, et al.Treadmill exercise alleviates prenatal noise stress-induced impairment of spatial learning ability through enhancinghippocampal neurogenesis in rat pups[J]. J Exerc Rehabil, 2013, 9(5):451-456.
[7] Chengzhi C, Yan T, Xuejun J, et al.Recovery of chronic noise exposure induced spatial learning and memory deficits in young male Sprague-Dawley rats[J]. J Occup Health, 2011, 53(3):157-163.
[8] 崔博, 吴铭权, 佘晓俊, 等. 噪声暴露对大鼠事件相关电位P300的影响及其海马水平的机制[J]. 中国应用生理学杂志, 2009, 3(25):404-407.
[9] 郑刚, 陈耀明, 张雪平, 等. 急性恐惧应激对大鼠行为、激素水平及脑Erk1/2活化表达的影响[J]. 中国应用生理学杂志, 2007, 23(3):270-274.
[10] Mo C, Pang TY, Ransome MI, et al.High stress hormone levels accelerate the onset of memory deficits in male Huntington's disease mice[J]. NeurobiolDis, 2014, 69:248-262.
[11] Rho JM, Storey TW. Molecular ontogeny of major neurotransmitter receptor systems in the mammalian central nervous system:norepinephrine, dopamine, serotonin, acetylcholine, and glycine[J]. J Child Neurol, 2001, 16(4):271-280.
[12] Bakay RAE, Ward AA. Enzymatic changes in serum and cerebrospinalfluid in neurological injury[J]. Collections, 2009, 116(6):27-37.
[13] Usui A, Kato K, Murase M, et al.Neural tissue-related proteins (NSE, G0 alpha, 28-kDa calbindin-D, S100b and CK-BB) in serum and cerebrospinal fluid after cardiac arrest[J]. J Neurol Sci, 1994, 123(1-2):134-139.
[14] 姜杰, 熊忠, 胡健饶. 神经肽与学习记忆[J]. 生物学通报, 2002,37(3):12-14.
[15] 高贵山, 于晓军, 徐小虎. 脑红蛋白与脑损伤研究现状[J]. 实用医学杂志, 2007, 23(5):765-767.
[16] Sun Y, Jin K, Peel A, et al.Neuroglobin protects the brainfrom experimental stroke in vivo[J]. Proc Natl Acad Sci. U.S A, 2003, 100(6):3497-3500.

强噪声对豚鼠认知功能和血清某些生化指标的影响

Effects of strong noise on cognitive function and some biochemical indexes in serum of guinea pigs

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