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中国应用生理学杂志 ›› 2016, Vol. 32 ›› Issue (5): 419-423.doi: 10.13459/j.cnki.cjap.2016.05.009

• 研究论文 • 上一篇    下一篇

蒙药新Ⅱ号对扩张型心肌病大鼠心功能、心肌细胞内质网应激及凋亡的作用

陈少青, 王伊林, 曹宪玉, 张青山, 柴花, 陶谢鑫, 赵明   

  1. 内蒙古民族大学附属医院, 通辽 028000
  • 收稿日期:2015-08-03 修回日期:2016-05-17 出版日期:2016-09-28 发布日期:2018-06-20
  • 通讯作者: 赵明,Tel:13474859991;E-mail:langzhe73@163.com E-mail:langzhe73@163.com
  • 基金资助:
    内蒙古自然科学基金资助课题(2016MS08125)

Effects of Mongolian new medicine-Ⅱ on cardiac functions, endoplasmic reticulum stress and apoptosis in congestive heart failure rats with dilated cardiomyopathy

CHEN Shao-qing, WANG Yi-lin, CAO Xian-yu, ZHANG Qing-shan, CHAI Hua, TAO Xie-xin, ZHAO Ming   

  1. The Inner-Mongolia National University Affiliated Hospital, Tongliao 028000, China
  • Received:2015-08-03 Revised:2016-05-17 Online:2016-09-28 Published:2018-06-20
  • Supported by:
    内蒙古自然科学基金资助课题(2016MS08125)

摘要: 目的:探讨蒙药新Ⅱ号对阿霉素性扩张型心肌病大鼠心功能、心肌病理、心肌细胞内质网应激及凋亡作用。方法:30 只SD 雄性大鼠分为3组(n=10): 正常对照组、模型组(腹腔注射阿霉素2 mg/kg体重,1次/周,用药4周后观察4周)和蒙药新Ⅱ号组(腹腔注射阿霉素2 mg/kg体重,1次/周,用药4周后,给予蒙药新Ⅱ号30 mg/kg·d灌胃,共4周)。观察大鼠的一般情况,8 周后行高频超声心动图检查,测定各项心功能指标,检查结束后处死大鼠,取心脏,行HE 染色、VG染色及电镜观察。用TUNEL 法检测各组大鼠心肌细胞凋亡, Western blot 检测各组大鼠心肌细胞内质网伴侣蛋白GRP78、GRP94及促凋亡因子CHOP、caspase-3表达。结果:与模型组相比较,①蒙药新Ⅱ号组大鼠心脏左室结构及血流动力学各项指标均有明显改善(P<0.05)。②蒙药新Ⅱ号组大鼠心肌病理积分明显下降(P<0.01)。③蒙药新Ⅱ号组大鼠心肌细胞内质网应激伴侣蛋白GRP78、GRP94、CHOP、caspase-3表达及细胞凋亡明显减少 (P<0.01)。结论:蒙药新Ⅱ号可改善扩张型心肌病大鼠心脏功能,减轻心肌病理变化,缓解内质网应激,减少心肌细胞凋亡。这一实验结果可能是蒙药新Ⅱ号治疗扩张型心肌病作用机制之一。

关键词: 蒙药新Ⅱ号, 扩张型心肌病, 内质网应激, 凋亡, 阿霉素, 大鼠

Abstract: Objective: To investigate the effects of Mongolian new medicine-Ⅱ oncardiac functions, myocardial pathology, endoplasmic reticulum stress and myocardial apoptosis in congestive heart failure with dilated cardiomyopathy in rats.Methods: Thirty SD male rats were randomly dividedinto 3 groups(n=10):control group, dilated cardiomyopathy group (intraperitoneal injection of adriamycin 2 mg/kg body weight, 1 time/week, 4 weeks after treatment were observed for 4 weeks), Mongolian new medicine-Ⅱ group(intraperitoneal injection of adriamycin 2 mg/kg body weight, 1 time/week, 4 weeks after treatment, 30 mg/(kg·d)was given Mongolian new medicine-Ⅱ orally for 4 weeks). During the experiment, general conditions of rats were observed. After 8 weeks, these rats were killed after measurement of the cardiac function indexes by high frequency echocardiography. The morphological changes of myocardial tissues were observed by using HEstaining, VG staining and electron microscopic. The myocardial apoptosis was detected by TUNEL method and the expressions of endoplasmic reticulum chaperone GRP78, GRP94, pro-apoptotic factor CHOP and caspase-3 were monitored by Western blot. Results: ① Compared with dilated cardiomyopathy group, the cardiac systolic and diastolic functions were significantly increased in Mongolian new medicine-Ⅱ group, which were reflected in that left ventricular contraction diameter(LVIDs) and left ventricular end-diastolic diameter(LVIDd) were decreased, and left ventricular shortening fraction(FS) and ejection fraction(EF) were increased. The hemodynamic parameters of rats were improved significantly in Mongolian new medicine-Ⅱ group. ②Compared with dilated cardiomyopathy group, the myocardial lesion score was decreased and fibrosis of tissue space was relieved in Mongolian new medicine-Ⅱ group. ③Compared with dilated cardiomyopathy group, the apoptosis of myocardial cells was decreased. ④The expressions of endoplasmic reticulum chaperone GRP78, GRP94, pro-apoptotic factor chop and caspase-3 were decreased in Mongolian new medicine-Ⅱ group. Conclusion: Mongolian new medicine-Ⅱ could improve the pathologic alterations of cardiac cells and cardiac functions, decrease endoplasmic reticulum stress, the degree of fibrosis and myocardial apoptosis. The experimental results may be one of the mechanisms of treatment function of Mongolian new medicine-Ⅱ on dilated cardiomyopathy.

Key words: Mongolian new medicine-Ⅱ, dilated cardiomyopathy, endoplasmic reticulum stress, apoptosis, adriamycin, rat

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