寒冷对雌性C57BL/6小鼠动情周期的影响*

doi:10.12047/j.cjap.5876.2019.098

中国应用生理学杂志 ›› 2019, Vol. 35 ›› Issue (5): 454-456.doi: 10.12047/j.cjap.5876.2019.098

寒冷对雌性C57BL/6小鼠动情周期的影响^*

赵海^1,2, 李曦²⁺, 张莉², 武帅², 张永强², 李军³, 张格祥^1△, 杨丹凤^2△

1. 兰州大学公共卫生学院, 甘肃兰州 730000;
2. 军事科学院军事医学研究院环境医学与作业医学研究所, 天津 300050;
3. 中国人民解放军32110部队卫生连, 黑龙江黑河 164300

收稿日期:2019-05-09 出版日期:2019-09-28 发布日期:2020-01-02
通讯作者: ^△Tel: 17627765932; E-mail: zhanggx@lzu.edu.cn, fengdyd@126.com
作者简介:⁺:为共同第一作者
基金资助:
BWS17J025;国家自然科学基金资助(21707165)

Effects of cold exposure on estrous cycle of female C57BL/6 mice

ZHAO Hai^1,2, LI Xi²⁺, ZHANG Li², WU Shuai², ZHANG Yong-qiang², LI Jun³, ZHANG Ge-xiang^1△, YANG Dan-feng^2△

1. School of Public Health, Lanzhou University, Lanzhou 730000;
2. Institute of Environmental and Operational Medicine, Academy of Military Medical Sciences, Academy of Military Sciences, Tianjin 300050;
3. Chinese PLA 32110, Heihe, China

Received:2019-05-09 Online:2019-09-28 Published:2020-01-02

摘要/Abstract

摘要： 目的:研究寒冷对雌性C57BL/6小鼠动情周期的影响。方法:12只雌性小鼠随机分为对照组、低温组,每组6只;低温组每天4℃暴露4 h,每天阴道涂片法观察小鼠动情状况,对照组饲养于常温动物房;每2 d称量体重,2周后心脏取血、子宫和卵巢,检测小鼠血清E2、FSH、LH、Prl、P水平,进行子宫、卵巢的组织病理学检查。结果:与对照组比较,低温组小鼠体重无显著性差异(P＞0.05),小鼠子宫脏器系数明显较低、动情间期明显延长(P＜0.01),血清FSH显著升高、Prl显著降低(P＜0.01),小鼠子宫腺管扩张,卵巢卵泡数量明显减少。结论:寒冷可使雌性C57BL/6小鼠动情周期延长,进而可能影响生殖功能。

关键词: 寒冷暴露, 动情周期, C57BL/6小鼠, 子宫, 卵巢

Abstract: Objective: To study the effects of cold exposure on estrous cycle of female C57BL/6 mice. Methods: Twelve healthy female C57BL/6 mice were randomly divided into two groups: control group and cold exposure group, 6 in each group. Cold exposure group was exposed to 4℃, 4 h per day, while control group stayed in normal conditions. Vaginal smears were used to observe the estrous cycle. After 2 weeks, blood and uteri were collected from each mouse after anesthetized and weighted. Serum levels of estradiol(E2), follicle-stimulating hormone(FSH), luteinizing hormone(LH), prolactin(Prl) and progesterone(P) were determined by using mouse ELISA kits. The uterus and ovary pathological slices were prepared to observe the structural changes. Results: Compared with the control group, body weight gain showed no significant differences (P＞0.05). The cold group had significant lower coefficients of uterus and the diestrus phase was significantly increased after cold exposure (P＜0.01). Serum level of FSH in cold group was higher and Prl was lower significantly (P＜0.01). Pathological examination of uterus and ovary showed that uterine glands of cold group were expanded and the amount of follicles was decreased significantly. Conclusion: Cold exposure might increase mouse estrous cycle and affect their reproductive function.

Key words: cold exposure, estrous cycle, C57BL/6 mice, uterus, ovary

赵海, 李曦, 张莉, 武帅, 张永强, 李军, 张格祥, 杨丹凤. 寒冷对雌性C57BL/6小鼠动情周期的影响^*[J]. 中国应用生理学杂志, 2019, 35(5): 454-456.

ZHAO Hai, LI Xi, ZHANG Li, WU Shuai, ZHANG Yong-qiang, LI Jun, ZHANG Ge-xiang, YANG Dan-feng. Effects of cold exposure on estrous cycle of female C57BL/6 mice[J]. CJAP, 2019, 35(5): 454-456.

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寒冷对雌性C57BL/6小鼠动情周期的影响^*

Effects of cold exposure on estrous cycle of female C57BL/6 mice

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