多巴胺对急性间歇性低氧诱导的大鼠颈动脉体低氧敏感性的影响*

doi:10.12047/j.cjap.6002.2020.091

中国应用生理学杂志 ›› 2020, Vol. 36 ›› Issue (5): 428-431.doi: 10.12047/j.cjap.6002.2020.091

多巴胺对急性间歇性低氧诱导的大鼠颈动脉体低氧敏感性的影响^*

贾祥磊^1,2, 刘玉珍^1,2△, 李超红^1,2, 范娅楠¹

1.新乡医学院第一附属医院, 2.河南省神经修复重点实验室, 新乡 453100

收稿日期:2020-01-06 修回日期:2020-06-04 发布日期:2021-02-25
通讯作者: ^△Tel: 0373-4403923; E-mail: yuzhenliu@xxmu.edu.cn
基金资助:
^*国家自然科学基金(81973988)

Effect of dopamine on the sensitivity of carotid body to hypoxia induced by acute intermittent hypoxia in rats

JIA Xiang-lei^1,2, LIU Yu-zhen^1,2△, LI Chao-hong^1,2, FAN Ya-nan¹

1. The First Affiliated Hospital of Xinxiang Medical University, 2. Henan Key Laboratory of Neural Regeneration, Xinxiang 453100, China

Received:2020-01-06 Revised:2020-06-04 Published:2021-02-25

摘要/Abstract

摘要： 目的: 观察急性间歇性低氧刺激后大鼠颈动脉体对低氧的敏感性以及多巴胺对颈动脉体低氧敏感性的影响。方法: 将分离SD大鼠的颈动脉体-窦神经移入到孵育槽,然后把分离的窦神经吸入到记录的玻璃电极中行电信号记录。记录基线部分缓冲液充入气体为95% O₂+ 5% CO₂混合气,低氧应激给予5% O₂+ 5% CO₂+ 90% N₂混合气,低氧刺激给予30 s,95% O₂+ 5% CO₂给予90 s,共10个循环,每组实验大鼠数量n大于等于5。结果: 大鼠离体的颈动脉体,给予急性间歇性低氧应激,再给予低氧刺激,窦神经较之前低氧刺激放电活动增强。但加入多巴胺后,可以抑制窦神经对低氧的反应,急性间歇性低氧后,多巴胺对窦神经的低氧放电活动抑制作用加强。结论: 大鼠颈动脉体给予急性间歇性低氧可增强窦神经对低氧的反应,多巴胺可抑制急性低氧诱导的颈动脉体对低氧敏感性的增强。

关键词: 颈动脉体, 急性间歇性低氧, 多巴胺, 可塑性, 大鼠

Abstract: Objective: To investigate the sensitivity of carotid body to hypoxia and the effect of dopamine on the sensitivity of carotid body to hypoxia after acute intermittent hypoxia stimulation in rats. Methods: The isolated carotid body-sinus nerve in rat was transferred to incubator, and then the isolated sinus nerve was inhaled into the recorded glass electrode for recording electrical signals. The baseline buffer was bubbled with 95% O₂+ 5% CO₂ mixture gas, and the hypoxic stress was treated with 5% O₂ + 5% CO₂ + 90% N₂ mixture gas, hypoxic stimulation was given for 30 seconds, 95% O₂ + 5% CO₂ for 90 seconds, a total of 10 cycles. No less than 5 rats in each group. Results: In this experiment, the electrical activity of sinus nerve isolated from rats was enhanced by hypoxia stimulation after acute intermittent hypoxia, but the response of sinus nerve to hypoxia was inhibited by dopamine. Before acute intermittent hypoxic stress, dopamine also inhibited the firing activity of sinus nerve, but after acute intermittent hypoxic cycle, the inhibition of dopamine on the firing activity of sinus nerve was strengthened. Conclusion: Acute intermittent hypoxia enhances the response of sinus nerve isolated from rats to hypoxia, dopamine inhibits the enhancement of carotid body sensitivity to hypoxia induced by acute intermittent hypoxic.

Key words: acute intermittent hypoxic, dopamine, plasticity, carotid body, rat

中图分类号:

Q423
Q424

贾祥磊, 刘玉珍, 李超红, 范娅楠. 多巴胺对急性间歇性低氧诱导的大鼠颈动脉体低氧敏感性的影响^*[J]. 中国应用生理学杂志, 2020, 36(5): 428-431.

JIA Xiang-lei, LIU Yu-zhen, LI Chao-hong, FAN Ya-nan. Effect of dopamine on the sensitivity of carotid body to hypoxia induced by acute intermittent hypoxia in rats[J]. CJAP, 2020, 36(5): 428-431.

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多巴胺对急性间歇性低氧诱导的大鼠颈动脉体低氧敏感性的影响^*

Effect of dopamine on the sensitivity of carotid body to hypoxia induced by acute intermittent hypoxia in rats

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