鳖甲育肝颗粒对乙醇性肝纤维化大鼠的影响*

doi:10.12047/j.cjap.6109.2020.122

中国应用生理学杂志 ›› 2020, Vol. 36 ›› Issue (6): 582-586.doi: 10.12047/j.cjap.6109.2020.122

鳖甲育肝颗粒对乙醇性肝纤维化大鼠的影响^*

韦凌霞, 丁茂鹏, 王志旺^△, 刘雪枫, 庞亚蓉, 郭玫

甘肃中医药大学, 兰州 730000

收稿日期:2020-06-09 修回日期:2020-11-25 出版日期:2020-11-28 发布日期:2021-03-15
通讯作者: ^△Tel: 0931-8765395; E-mail: wzw0933@126.com
基金资助:
^*国家自然科学基金项目(81860787);甘肃省高等学校科研项目(2015A-096)

Effects of Biejia Yugan Granule on rat with ethanol-induced hepatic fibrosis

WEI Ling-xia, DING Mao-peng, WANG Zhi-wang^△, LIU Xue-feng, PANG Ya-rong, GUO Mei

Gansu College of Traditional Chinese Medicine, Lanzhou 730000, China

Received:2020-06-09 Revised:2020-11-25 Online:2020-11-28 Published:2021-03-15

摘要/Abstract

摘要： 目的:探讨鳖甲育肝颗粒对乙醇性肝纤维化大鼠的防治作用。方法:将SD大鼠随机分为空白对照组、模型对照组、鳖甲育肝颗粒对照组、秋水仙碱组、鳖甲育肝颗粒低剂量组和鳖甲育肝颗粒高剂量组(n=8),采用梯度乙醇灌胃法复制肝纤维化动物模型,即除了空白对照组及鳖甲育肝颗粒对照组灌胃等量纯净水外,其余各组大鼠第1～4周灌胃5 g/(kg·d)乙醇,第5～8周灌胃7 g/(kg·d)乙醇,第9～12周灌胃9 g/(kg·d)乙醇,第13～24周灌胃9.5 g/(kg·d)乙醇,其中乙醇剂量计算采用下列公式:乙醇剂量(g) = 无水乙醇体积(ml)×预配乙醇浓度(%)×0.8(g/ml),同时每天灌胃相应的药物:鳖甲育肝颗粒对照组灌胃鳖甲育肝颗粒5.55 g/kg、秋水仙碱组灌胃秋水仙碱0.1 mg/kg、鳖甲育肝颗粒低剂量组灌胃鳖甲育肝颗粒1.85 g/kg、鳖甲育肝颗粒高剂量组灌胃鳖甲育肝颗粒5.55 g/kg,空白对照组及模型对照组灌胃等量纯净水。实验第169日观测鳖甲育肝颗粒对大鼠肝脏脏器宏观变化、肝组织含水量及其纤维化病理变化、肝组织羟脯氨酸(Hyp)含量及α-平滑肌肌动蛋白(α-SMA)、CREB表达水平的影响。结果:1.85、5.55 g/kg鳖甲育肝颗粒能明显改善乙醇性肝纤维化大鼠肝脏脏器宏观变化及肝组织纤维化病理变化,降低肝组织含水量及Hyp的含量,下调α-SMA、CREB表达水平。结论:鳖甲育肝颗粒具有明显的抑制乙醇性肝纤维化的作用,而下调CREB是其抗肝纤维化的作用机制之一。

关键词: 鳖甲育肝颗粒, 乙醇性肝纤维化, 大鼠, 防治作用, 环磷腺苷效应元件结合蛋白

Abstract: Objective: To investigate the prevention and treatment effect of Biejia Yugan Granule (BYG) on ethanol-induced hepatic fibrosis (EHF) rats. Methods: SD rats were randomly divided into blank control group, model group, BYG group, colchicine group and BYG low and BYG high dose groups (n=8). The EHF rat model was established by intragastric edible ethanol with a gradually increased dose. Briefly, the rats of model group, colchicine group and BYG low and high dose groups were given gavage of 5 g/(kg·d) ethanol at week 1～4, 7 g/(kg·d) ethanol at week 5～8, 9 g/(kg·d) ethanol at week 9～12 and 9.5 g/(kg·d) ethanol at week 13～24. And the other two groups were treated with equal volume water. At the same time, the corresponding drugs were administrated daily: BYG group was treated with Biejia Yugan Granules 5.55 g/kg, colchicine group was treated with colchicine 0.1 mg/kg, BYG low-dose and high-group were treated with Biejia Yugan Granules 1.85 and 5.55 g/kg respectively. The blank control group and model control group were given the same amount of purified water. On the 169th day of the experiment, the effects of BYG on the macroscopic changes of rat liver organs, the water content of liver tissue and the pathological changes of fibrosis, the content of hydroxy proline (Hyp) in liver tissue and the expression levels of α-SMA and CREB were observed. Results: BYG at the doses of 1.85 and 5.55 g/kg could significantly improve the macroscopic changes of liver and pathological changes of liver tissue fibrosis in rats with EHF, reduce the contents of water and Hyp in liver tissue, and down-regulate the expressions of α-SMA and CREB. Conclusion: BYG has obvious effect on inhibiting EHF and one of its mechanisms is down-regulate the content of CREB.

Key words: Biejia Yugan Granule (BYG), ethanol-induced hepatic fibrosis (EHF), rat, prevention and treatment effect, cAMP-response element binding protein (CREB)

中图分类号:

R285.5

韦凌霞, 丁茂鹏, 王志旺, 刘雪枫, 庞亚蓉, 郭玫. 鳖甲育肝颗粒对乙醇性肝纤维化大鼠的影响^*[J]. 中国应用生理学杂志, 2020, 36(6): 582-586.

WEI Ling-xia, DING Mao-peng, WANG Zhi-wang, LIU Xue-feng, PANG Ya-rong, GUO Mei. Effects of Biejia Yugan Granule on rat with ethanol-induced hepatic fibrosis[J]. CJAP, 2020, 36(6): 582-586.

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鳖甲育肝颗粒对乙醇性肝纤维化大鼠的影响^*

Effects of Biejia Yugan Granule on rat with ethanol-induced hepatic fibrosis

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