首页  期刊介绍 征稿简则 编委会 期刊征订 广告服务 留言板 联系我们 English

中国应用生理学杂志 ›› 2020, Vol. 36 ›› Issue (6): 600-604.doi: 10.12047/j.cjap.6041.2020.126

• 研究论文 • 上一篇    下一篇

miR-203a靶向及其靶基因对乳腺癌淋巴结转移的影响*

韩利蓉1, 宋江勤1, 郭飞波1, 张栋武2△   

  1. 1.湖北省天门市第一人民医院, 天门 431700;
    2.广东医科大学附属佛山高明医院, 佛山 528500
  • 收稿日期:2020-03-17 修回日期:2020-11-25 出版日期:2020-11-28 发布日期:2021-03-15
  • 通讯作者: Tel: 13026770585; E-mail: gdpueducn@126.com

Effects of miR-203a targeting and its target gene on lymph node metastasis of breast cancer

HAN Li-rong1, SONG Jiang-qin1, GUO Fei-bo1, ZHANG Dong-wu2△   

  1. 1. Hubei Tianmen First People's Hospital, Tianmen 431700;
    2. Foshan Gaoming Hospital affiliated to Guangdong Medical University, Foshan 528500, China
  • Received:2020-03-17 Revised:2020-11-25 Online:2020-11-28 Published:2021-03-15

摘要: 目的:探讨miR-203a靶向及其靶基因ATM在乳腺癌组织中的表达及相关性,为乳腺癌的发病机制尤其是淋巴结转移机制提供理论依据。方法:收集30例配对的乳腺癌和癌旁正常组织,对两组标本采用RT-qPCR检测miR-203a及ATM的相对表达量,对miR-203a和ATM进行相关分析,并对其与临床病理特征进行相关分析,比较miR-203a和ATM的表达在淋巴结转移和未转移之间是否有统计学差异。结果:与癌旁正常组织相比,乳腺癌组织中miR-203a的表达显著升高(P<0.01),ATM的表达显著降低(P<0.01),二者呈显著负相关(r=-0.847,P< 0.01);miR-203a和ATM的表达均与淋巴结是否转移与不同临床分期显著相关(P<0.05);miR-203a在淋巴结已转移组中的表达显著低于未转移组(P<0.05),ATM在淋巴结已转移组中的表达显著高于未转移组(P<0.01)。结论:乳腺癌早期miR-203a过表达抑制其靶基因ATM的表达很可能是一种调节肿瘤细胞增殖、转移和侵袭性的保护机制,到中晚期下调miR-203a上调ATM基因,可能参与淋巴结转移。

关键词: miR-203a, ATM , 靶基因, 乳腺癌, 淋巴结转移

Abstract: Objective: To study the expression and correlation of mir-203a and its target gene ATM in breast cancer tissues, so as to provide theoretical basis for the pathogenesis of breast cancer, especially lymph node metastasis. Methods: Thirty paired breast cancer and paracancer normal tissues were collected, and RT-qPCR was used to detect the relative expression levels of mir-203a and ATM in the samples of the two groups. Correlation analysis was conducted for mir-203a and ATM, and correlation analysis was conducted for the pathological characteristics, so as to compare whether there were statistical differences between mir-203a and ATM in lymph node metastasis and non-metastasis. Results: Compared with normal paracancer tissues, the expression level of mir-203a in breast cancer tissues was significantly increased (P<0.01), and the expression level of ATM was significantly decreased (P<0.01), showing a significant negative correlation between the two tissues (r=-0.847,P<0.01).The expression level of mir-203a and ATM was significantly correlated with lymph node metastasis and different clinical stages (P<0.05). The expression level of mir-203a in the group with lymph node metastasis were significantly lower than that in the group without lymph node metastasis (P<0.05), and the expression of ATM in the group with lymph node metastasis was significantly higher than that in the group without lymph node metastasis (P<0.01). Conclusion: The overexpression of mir-203a in the early stage of breast cancer may inhibit the expression of its target gene ATM, which may be a protective mechanism to regulate the proliferation,metastasis and invasiveness of tumor cells. In the middle and late stage, mir-203a is down-regulated and the ATM gene is up-regulated, which may be involved in lymph node metastasis.

Key words: MiR-203a, ATM, target genes, breast cancer, lymph node metastasis

中图分类号: 

版权所有 © 2015 《中国应用生理学杂志》编辑部
京ICP备16058274号-1
地址:天津市和平区大理道1号,邮编:300050  电话:022-84655184  E-mail:tjzgyish@163.com
本系统由北京玛格泰克科技发展有限公司设计开发 技术支持:support@magtech.com.cn