不同中药复方及不同提取方法提取液对小鼠耐缺氧作用的影响*

doi:10.12047/j.cjap.6227.2022.039

中国应用生理学杂志 ›› 2022, Vol. 38 ›› Issue (3): 199-204.doi: 10.12047/j.cjap.6227.2022.039

不同中药复方及不同提取方法提取液对小鼠耐缺氧作用的影响^*

李婉玉^1,2, 马慧萍^1,3, 马趣环², 石晓峰^1,2△, 鲁艳梅^1,2, 张朋朋^1,3, 张家旭^1,2, 董学凤^1,2, 叶倩女^1,2

1.甘肃中医药大学, 兰州 730030;
2.甘肃省医学科学研究院, 兰州 730050;
3.中国人民解放军联勤保障部队第九四〇医院全军高原损伤防治重点实验室, 甘肃兰州 730050

收稿日期:2021-11-29 修回日期:2022-04-01 出版日期:2022-05-28 发布日期:2022-09-05
通讯作者: ^△Tel: 0931-2302664; E-mail: shixiaofeng2005@sina.com
基金资助:
^*2017年度甘肃省兰州市人才创新创业项目(2017-RC-28)

The effects of different herbal compound and extracts from different extraction methods on hypoxia tolerance in mice

LI Wan-yu^1,2, MA Hui-ping^1,3, MA Qu-huan², SHI Xiao-feng^1,2△, LU Yan-mei^1,2, ZHANG Peng-peng^1,3, ZHANG Jia-xu^1,2, DONG Xue-feng^1,2, YE Qian-nju^1,2

1. Gansu University of Traditional Chinese Medicine, Lanzhou 730030;
2. Gansu Academy of Medical Sciences, Lanzhou 730050;
3. Ninth Joint Logistics Support Unit, Chinese People's Liberation Army Key Laboratory for Prevention and Treatment of Plateau Injury of the Chinese People's Liberation Army, Gansu Lanzhou 730050, China

Received:2021-11-29 Revised:2022-04-01 Online:2022-05-28 Published:2022-09-05

摘要/Abstract

摘要： 目的: 探讨不同中药处方组成及其不同提取方法的复方提取物对小鼠耐缺氧能力的影响,以优选其处方组成和制备提取方法。方法: 将雄性BALB/c小鼠随机分成6组:空白对照组、复方丹参组、复方红景天醇-水提取组(红景天、黄芪、黄精、枸杞子)、复方红景天水提取组、复方黄芪醇-水提取组(黄芪、黄精、枸杞子)、复方黄芪水提取组,每组30只,每组小鼠连续灌胃给药10 d,空白组灌胃灭菌注射用水,复方丹参组0.15 g/kg,复方红景天醇-水提取组和水提取组3 g/kg,复方黄芪醇-水提取组和水提取组1.7 g/kg。各组于末次灌胃1 h后进行常压耐缺氧实验、亚硝酸钠中毒存活实验和急性脑缺血缺氧实验,并测定小鼠脑组织氧化应激相关抗氧化物酶活性和代谢物含量。结果: 与空白对照组相比,复方丹参组、复方黄芪醇-水提取组和水提取组常压耐缺氧存活时间均显著延长(P＜0.01),脑缺血缺氧后张口喘气次数均显著增加(P＜0.05)。各组注射亚硝酸钠后存活时间没有统计学差异。与空白对照组相比,复方黄芪水提取组T-AOC、SOD、GSH和CAT活性均显著升高(P＜0.05,P＜0.01),MDA含量均显著降低(P＜0.01),与复方丹参组相比,复方黄芪水提取组SOD、CAT、GSH活性均显著升高(P＜0.01,P＜0.05),MDA含量显著降低(P＜0.05)。结论: 复方黄芪水提取组的耐缺氧效果最好,原复方红景天全方可以剔除红景天由黄芪、黄精、枸杞子组成,其提取方法为水提。

关键词: 复方红景天, 不同组成, 不同提取方法, 耐缺氧作用

Abstract: Objective: To investigate the effects of different prescription compositions of traditional Chinese medicine and its different extraction methods of compound formula extracts on hypoxia tolerance in mice, in order to preferably select their prescription compositions and preparation extraction methods. Methods: Male BALB/c mice were randomly divided into 6 groups: blank control group, compound danshen group, compound Rhodiola Rosea alcohol-water extract group (Rhodiola rosea, Astragali Radix, Polygonati Rhizoma, Lycii Fructus), compound Rhodiola Rosea water extract group, compound Astragalus alcohol-water extract group (Astragali Radix, Polygonati Rhizoma, Lycii Fructus) and compound Astragalus water extract group, 30 mice in each group. Each group was administered continuously by gavage for 10 d. The blank group was gavaged with sterilized injection water. The mice in the other groups were treated with 0.15 g/kg of compound danshen, 3 g/kg of compound Rhodiola Rosea alcohol-water extract or water extract, and 1.7 g/kg of compound Astragalus alcohol-water extract or water extract, respectively. Each group was subjected to normobaric hypoxia tolerance test, sodium nitrite toxicity survival test and acute cerebral ischemia-hypoxia test 1 h after the last gavage, and the mice brain tissues were used to determine the activity of antioxidant enzymes and metabolites related to oxidative stress. Results: Compared with the blank control group, in normobaric hypoxia tolerance test, the survival time of mice in the compound danshen group and the compound Astragalus alcohol-water extract group and water extraction group was prolonged significantly (P＜0.01), and the number of open-mouth gasping after cerebral ischemia and hypoxia was increased significantly (P＜0.05). There was no statistical difference in survival time after sodium nitrite injection in each group. Compared with the blank control group, the activities of T-AOC, SOD, GSH and CAT were increased significantly (P＜0.05, P＜0.01) and the content of MDA was decreased significantly (P＜0.01) in the compound Astragalus water extract group. Compared with the compound danshen group, the activities of SOD, CAT and GSH were increased significantly (P＜0.01, P＜0.05) and the content of MDA was decreased significantly (P＜0.05). Conclusion: Compound Astragalus water extraction has the best effect of hypoxia tolerance, compound Rhodiola Rosea can eliminate Rhodiola rosea and consists of Astragali Radix, Polygonati Rhizoma, Lycii Fructus and its extraction method is water extraction.

Key words: compound Rhodiola Rosea, different grouping, different extraction methods, hypoxia tolerance

中图分类号:

R285.5

李婉玉, 马慧萍, 马趣环, 石晓峰, 鲁艳梅, 张朋朋, 张家旭, 董学凤, 叶倩女. 不同中药复方及不同提取方法提取液对小鼠耐缺氧作用的影响^*[J]. 中国应用生理学杂志, 2022, 38(3): 199-204.

LI Wan-yu, MA Hui-ping, MA Qu-huan, SHI Xiao-feng, LU Yan-mei, ZHANG Peng-peng, ZHANG Jia-xu, DONG Xue-feng, YE Qian-nju. The effects of different herbal compound and extracts from different extraction methods on hypoxia tolerance in mice[J]. CJAP, 2022, 38(3): 199-204.

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不同中药复方及不同提取方法提取液对小鼠耐缺氧作用的影响^*

The effects of different herbal compound and extracts from different extraction methods on hypoxia tolerance in mice

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