发育中小鼠运动皮层锥体神经元电生理特性的变化*

doi:10.12047/j.cjap.6304.2022.091

中国应用生理学杂志 ›› 2022, Vol. 38 ›› Issue (5): 485-490.doi: 10.12047/j.cjap.6304.2022.091

发育中小鼠运动皮层锥体神经元电生理特性的变化^*

白天宇¹, 段红梅², 张博雅², 郝鹏², 郝飞¹, 高钰丹², 赵文², 杨朝阳², 李晓光^1,2,△

1.北京航空航天大学生物与医学工程学院, 北京 100083;
2.首都医科大学神经生物学系, 北京 100069

收稿日期:2022-03-16 修回日期:2022-08-22 出版日期:2022-09-28 发布日期:2023-04-23
通讯作者: ^△Tel: 13501092601; E-mail: lxgchina@sina.com
基金资助:
^*国家自然科学基金重点项目(31730030);国家自然科学基金专项项目(81941011);国家自然科学基金面上项目(31971279,31771053);国家自然科学基金青年项目(31900749);北京市科技计划课题(Z181100001818007)

Changes in electrophysiological properties of pyramidal neuron in motor cortex during the postnatal early development of mice

BAI Tian-yu¹, DUAN Hong-mei², ZHANG Bo-ya², HAO Peng², HAO Fei¹, GAO Yu-dan², ZHAO Wen², YANG Zhao-yang², LI Xiao-guang^1,2

1. School of Biological Science and Medical Engineering, Beihang University, Beijing 100083;
2. Department of Neurobiology, Capital Medical University, Beijing 100069, China

Received:2022-03-16 Revised:2022-08-22 Online:2022-09-28 Published:2023-04-23

摘要/Abstract

摘要： 目的: 研究小鼠生后发育过程中运动皮层锥体神经元电生理特性的变化。方法: 选取出生后不同发育阶段的小鼠共计36只,随机分为1、2、3周龄组(1-, 2-, 3-Week)、1、2、3月龄组(1-, 2-, 3-Month ) (n=6)。应用全细胞膜片钳及生物胞素细胞内标记技术区分锥体神经元与中间神经元,同时记录各组小鼠脑片运动皮层锥体神经元的被动膜特性、动作电位(AP)及兴奋性突触后电流(sEPSCs)。结果: 与中间神经元相比,小鼠运动皮层锥体神经元的AP放电特征表现为规则放电(RS),放电频率较为缓慢。小鼠运动皮层锥体神经元的被动膜特性在出生后发育期间表现为:与1周龄组小鼠相比,2周龄组的静息膜电位(RMP)表现为显著超极化(P＜0.01),2周后再无明显改变;1月龄组的膜输入阻抗(R_in)呈现显著下降的趋势(P＜0.01),在1月龄后无明显变化;膜电容(Cm)无明显变化。 AP在发育早期的变化表现为:与1周龄组小鼠相比,3周龄组AP阈电位绝对值和幅值显著增加(P＜0.01),2周龄组AP半波宽显著降低(P＜0.05),在此之后无显著变化。 sEPSCs随发育过程的变化表现为:1月龄小鼠sEPSCs的频率和幅值相较于1周龄组均显著增加(P＜0.01),在1月后趋于稳定,无显著差异。结论: 在小鼠出生后发育过程中,运动皮层锥体神经元电生理特性的变化存在时间特异性,电生理特性的成熟程度可以作为检测神经元是否成熟的功能学指标,同时电生理特性可以作为皮质中间神经元与锥体神经元区分的标志。

关键词: 小鼠, 运动皮层, 发育, 全细胞膜片钳, 被动膜特性, 动作电位, 兴奋性突触后电流

Abstract: Objective: To investigate the electrophysiological properties of pyramidal neurons in mouse motor cortex during the early postnatal development. Methods: Thirty-six mice were randomly divided into postnatal 1-, 2-, 3-Week and 1-, 2-,3-Month groups (n=6). Membrane properties, action potentials (AP) and spontaneous excitatory postsynaptic currents (sEPSCs) of motor cortex pyramidal neurons were recorded to evaluate the changes in the intrinsic electrophysilogical characteristics by using whole cell patch clamp. Pyramidal neurons and interneurons were distinguished according to the AP firing patterns. Results: Comparing with interneurons, pyramidal neurons exhibited regular spiking (RS) with smaller frequency. During the period of postnatal 1 Week-3 Months, some of the intrinsic membrane properties of motor cortex pyramidal neurons changed. Compared to the 1-Week mice, the resting membrane potential (RMP) of 2-Week decreased significantly (P＜0.01), and the membrane input resistance (R_in) of 1-Month got a hyperpolarization (P＜0.01), and they showed no significant change in the next period, while the membrane capacitance (Cm) showed no significant changes during the whole postnatal development. The AP dynamic properties changed significantly during this period. Compared to the 1-Week mice, the absolute value of the AP threshold and the AP amplitude of the 3-Week increased significantly (P＜0.01), while the spike half width of the 2-Week decreased substantially (P＜0.05), and they showed no significant change in the next period. The sEPSCs frequency and amplitude of 1- Month increased significantly compared to the 1-Week mice(P＜0.01), while during the period of next 1 Month-3 Months, the amplitude and frequency showed no significant change. Conclusion: These results suggest that the motor cortex pyramidal neurons have time-specific eletrophysilogical properties during the postnatal development. The electrophysiological properties can be used as a functional index to detect the degree of neurons maturity, and as a marker to distinguish the pyramidal neurons and interneurons.

Key words: mice, motor cortex, development, whole cell patch clamp, membrane properties, action potentials, spontaneous excitatory postsynaptic currents

中图分类号:

R338

白天宇, 段红梅, 张博雅, 郝鹏, 郝飞, 高钰丹, 赵文, 杨朝阳, 李晓光. 发育中小鼠运动皮层锥体神经元电生理特性的变化^*[J]. 中国应用生理学杂志, 2022, 38(5): 485-490.

BAI Tian-yu, DUAN Hong-mei, ZHANG Bo-ya, HAO Peng, HAO Fei, GAO Yu-dan, ZHAO Wen, YANG Zhao-yang, LI Xiao-guang. Changes in electrophysiological properties of pyramidal neuron in motor cortex during the postnatal early development of mice[J]. CJAP, 2022, 38(5): 485-490.

参考文献

[1] Feldmeyer DR, Radnikow G. Developmental alterations in the functional properties of excitatory neocortical synapses[J]. J Physiol, 2009, 587(9): 1889-1896.
[2] Lohmann C, Kessels HW. The developmental stages of synaptic plasticity[J]. J Physiol, 2014, 592(1): 13-31.
[3] Arlotta P, Molyneaux BJ, Chen J, et al. Neuronal subtype-specific genes that control corticospinal motor neuron development in vivo[J]. Neuron, 2005, 45(2): 207-221.
[4] Elston GN, Fujita I. Pyramidal cell development: postnatal spinogenesis, dendritic growth, axon growth, and electrophysiology[J]. Front Neuroanat, 2014, 8: 78-98.
[5] Valiullina F, Akhmetshina D, Nasretdinov A, et al. Developmental changes in electrophysiological properties and a transition from electrical to chemical coupling between excitatory layer 4 neurons in the rat barrel cortex[J]. Front Neural Circuits, 2016, 10: 1-16.
[6] Ammer JJ, Grothe B, Felmy F. Late postnatal development of intrinsic and synaptic properties promotes fast and precise signaling in the dorsal nucleus of the lateral lemniscus[J]. J Neurophysiol, 2012, 107(4): 1172-1185.
[7] Etherington SJ, Williams SR. Postnatal development of intrinsic and synaptic properties transforms signaling in the layer 5 excitatory neural network of the visual cortex[J]. J Neurosci, 2011, 31(26): 9526-9537.
[8] Kroon T, Van Hugte E, Van Linge L, et al. Early postnatal development of pyramidal neurons across layers of the mouse medial prefrontal cortex[J]. Sci Rep, 2019, 9(1): 1-16.
[9] Chen XY, Zhang AF, Zhao W, et al. Electrophysiological characteristics of hippocampal postnatal early development mediated by AMPA receptors in rats. [J]. Acta Physiol Sin, 2018, 70(2): 106-114.
[10] Huggenberger S, Vater M, Deisz RA. Interlaminar differences of intrinsic properties of pyramidal neurons in the auditory cortex of mice[J]. Cereb Cortex, 2009, 19(5): 1008-1018.
[11] Boldog E, Bakken TE, Hodge RD, et al. Transcriptomic and morphophysiological evidence for a specialized human cortical GABAergic cell type[J]. Nat Neurosci, 2018, 21(9): 1185-1195.
[12] Patricia PG, Ricardo PD, Noelia GD, et al. Refinement of active and passive membrane properties of layer V pyramidal neurons in rat primary motor cortex during postnatal development[J]. Front Mol Neurosci, 2021, 14: 1-14.
[13] 刘文峰, 刘少鹏, 傅让, 等. 耐力运动对增龄大鼠脑皮层突触可塑性的影响及相关调控机制[J]. 中国应用生理学杂志, 2019, 35(4): 339-345.
[14] 胡琰茹, 刘晓莉, 乔德才. 一次性力竭运动过程中大鼠“黑质—丘脑—皮层”通路神经元相干性及递质动态变化[J]. 中国应用生理学杂志, 2017, 33(3): 204-211.
[15] Kaneko K, Koyanagi Y, Oi Y, et al. Propofol-induced spike firing suppression is more pronounced in pyramidal neurons than in fast-spiking neurons in the rat insular cortex[J]. Neuroscience, 2016, 339: 548-560.
[16] Aller MI, Wisden W. Changes in expression of some two-pore domain potassium channel genes (KCNK) in selected brain regions of developing mice[J]. Neuroscience, 2008, 151(4): 1154-1172.
[17] Moody WJ, Bosma MM. Ion channel development, spontaneous activity, and activity-dependent development in nerve and muscle cells[J]. Physiol Rev, 2005, 85(3): 883-941.
[18] Eyal G, Mansvelder HD, De Kock CP, et al. Dendrites impact the encoding capabilities of the axon[J]. J Neurosci, 2014, 34(24): 8063-8071.
[19] Jin Z, Choi MJ, Park CS, et al. Propofol facilitated excitatory postsynaptic currents frequency on nucleus tractus solitarii (NTS) neurons[J]. Brain Res, 2012, 1432: 1-6.
[20] Spigelman I, Zhang L, Carlen PL. Patch-clamp study of postnatal development of CA1 neurons in rat hippocampal slices: membrane excitability and K+ currents[J]. J Neurophysiol, 1992, 68(1): 55-69.

发育中小鼠运动皮层锥体神经元电生理特性的变化^*

Changes in electrophysiological properties of pyramidal neuron in motor cortex during the postnatal early development of mice

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