Home  About Journal Instructions for Authors Editorial Board Subscribe Advertisement Messages Board Contact Us 中文

CJAP ›› 2017, Vol. 33 ›› Issue (2): 174-178.doi: 10.12047/j.cjap.5458.2017.044

Previous Articles     Next Articles

The research of the progesterone and estrogen secretion level and the FSHR, LHR gene quantitative in granular cells cultured in vitro in different time of laying hens

LI Yue1, JI Hong1, XUE Lin-lin2, MA Li1, YANG Huan-min1, LIAN Shuai1, GUO Wen-jin1, ZHANG YU-chen1, ZHAI Jun-fei1, GUO Jing-ru1, LIU Yan-zhi1, ZHEN Li1   

  1. 1. College of Animal Science and Technology, Heilongjiang Bayi Agricultural University, Daqing 163319;
    2. Heilongjiang Polytechnic, Shuangcheng 150111, China
  • Received:2016-05-26 Revised:2016-11-28 Online:2017-03-28 Published:2018-06-20
  • Supported by:
    国家自然科学基金项目(31302052);黑龙江省自然科学基金项目(ZD201116)

Abstract: Objective: To research the hormone secretion levels of progesterone and estrogen and the gene expression levels of two go-nadotropin receptors follicle stimulating hormone receptor (FSHR) and luteinizing hormone receptor (LHR) in granular cells of laying hen, and the effect of culture time on the levels of hormone secretion and expression of related receptor gene in granulosa cells was inferred. Methods: The experiment using the method of cells culture in vitro, the granular cells supernatants of hens were collected at 0 h, 24 h, 48 h, 72 h, 96 h, the progesterone and estrogen concentrations in cell supernatants were determined by ELISA kits, and detected the expression of FSHR and LHR gene in granular cells by real-time fluorescent quantitative PCR. Results: The results showed that the progesterone and estrogen secretion reduced in the early culture of 0 h~48 h(P < 0.05), with the culture time increases to 72 h, the secretion of two hormones began to in-crease, and reaching the level of the initial level of culture. When the cells were cultured to 96 h, the rogesterone and estrogen secretion was reduced again. The lower levels of FSHR and LHR mRNA expression in granular cells appeared with the increase of culture time, compared with the group of cell culture to 0 h, the mRNA expression levels of each groups reduced obviously(P < 0.05). Conclusion: The amount of progesterone and estrogen in the cultured follicular granulosa cells decreased with the increase of in vitro culture time, and then increased. This might be related to the growth state of cells cultured in vitro. But on the whole, with the extension of the training time, the secretion of proges-terone and estrogen in the cells decreased. This may be related to the decreased expression of the FSHR and LHR genes in the two go-nadotropin receptors.

Key words: laying hens, granular cells, progesterone, estrogen, follicle-stimulating hormone receptor, luteinizing hormone receptor

Copyright © 2015 CJAP, All Rights Reserved.
Powered by Beijing Magtech Co. Ltd