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  • Table of Content
      28 March 2017, Volume 33 Issue 2 Previous Issue    Next Issue
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    Effects of COS on promoting the pathological ovarian aging mice ovarian function by regulating immune function
    LI Xiao-yan, CAO Xuan, LIU Xin-yue, YE Hai-feng, SU Tie, ZHENG Tuo-chen, MAO Zi, ZHENG Yue-hui
    CJAP. 2017, 33 (2): 97-102.   DOI: 10.12047/j.cjap.5457.2017.025
    Abstract   PDF (2121KB) ( 13 )
    Objective: To determine whether the immunopotentiator chitosan oligosaccharide(COS)can recover the reproductive functions of pathological ovarian recession mice and improvetheir immunity. Methods: Forty-three fertile female mice (at around 2 months),in addition to a normal control group (n=8), injected intraperitoneally with busulfan and cyclophosphamide to construct premature ovarian failure mod-els. Three of them were used to test whether the infertility model was constructed successfully by HE staining. Then the models were randomly divided into four groups (n=8) and treated with different dosages of COS by gavage, after which compared different groups' organ ratios (the weight of immune organs and ovary/body weight), ovarian follicles and peritoneal macrophages' phagocytosis as well as estragon(E2) and pro-gesterone(P) levels in peripheral blood. In addition, we measured the expression dynamics of the ovarian protein reproductive cell marker mouse vasa homolog(MVH), germ stem cell marker OCT-4 in ovarian surface epithelium (OSE) and part of immune factors including tumor necrosis factor (TNF-α),interleukin-2(IL-2)as well as IL-6 to analyze the correlativity between germline stem cells marker dynamics and im-mune factors expression changes. Results: With increasing dosages of COS, organ ratios of ovaries, thymus and spleen both went up syn-chronously; The whole number of follicles and every stages of follicles are all presenting with progressive tendency; E2 level in peripheral blood ascends, however, progesterone level declined relatively; Neutral red experiment revealed the phagocytosis ability of peritoneal macrophages became stronger with increasing dosages of COS; the results of Western blot had shown that no matter the expression level of germ stem cells marker or immune factors were all presenting increasing tendency, which means that the expression level dynamics of germ stem cell marker has a positive correlation with immune factors expression changes. The results were statistically significant. Conclusion: COS could improve the immunity of mice with pathological ovarian recession and at the same time it would promote the proliferation and differentiation of female germ line stem cells (FGSCs), and then helped saving ovarian functionsto some extent.
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    Effects of oxygen-glucose deprivation on the permeability of human umbilical vein endothelial cells
    HAN Xue, HUANG Xin, ZHU Ling-ling, FAN Ming
    CJAP. 2017, 33 (2): 105-108.   DOI: 10.12047/j.cjap.5407.2017.027
    Abstract   PDF (1194KB) ( 130 )
    Objective: To investigate the effects of oxygen-glucose deprivation (OGD) on the functions of blood-brain barrier in vitro, we established an OGD model to mimic cerebral ischemic injury in human umbilical vein endothelial cells (HUVECs). Methods: Cells were cul-tured in DMEM without glucose under hypoxic conditions (0.3% O2) for different time point. The survival rate of HUVECs was detected by CCK-8 assay. The permeability of HUVECs was measured by epithelial volt-ohm meter. The expression of tight junction protein was detected by Western blot analysis. Results: In OGD group, the survival rate of HUVECs was obviously decreased. The permeability of HUVECs also decreased in OGD-treated cells. And the expression of tight junction protein was reduced after OGD treatment. Conclusion: Our data indicate that OGD treatment disrupt the barrier function of tight junctions and increase permeability of HUVECs and finally accelerated cell death.
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    Protective effects of Genistein on human renal tubular epithelial cells damage of microwave radiation
    MA Qiang, WANG Hong-fei, BAO Jun-qiang, WU Ye-bin, WEN Jing, QI Yun, GONG Mei-liang
    CJAP. 2017, 33 (2): 109-111.   DOI: 10.12047/j.cjap.5382.2017.028
    Abstract   PDF (1003KB) ( 38 )
    Objective: To observe the effects of microwave irradiation on human proximal renal tubular epithelial cells (HKC) and protec-tive effects of genistein. Methods: HKC cells were divided into control group, microwave irradiation group and genistein group(n=6) re-spectively. The genistein group cells were pre-incubated with 30μmol/L genistein in DMEM for 2 hours. After irradiation for 24 hours, the concentrations of lactate dehydrogenase(LDH) and β-N-acetyl glucosaminidase(NAG) in culture solution were measured to evaluate cell injury. Cells were curetted to measure the levels of malondialdehyde (MDA) and superoxide dismutase (SOD). Cell apoptosis and necrosis were de-tected with Hoechst 33258 stain. Results: Compared with the control group, the NAG activity of the microwave irradiation group was signifi-cantly increased(P < 0.01), and NAG activity of genistein pre-incubated group was significantly decreased(P < 0.01). The levels of LDH in microwave irradiation group were also increased significantly (P < 0.01 vs control group). LDH levels could be decreased obviously (P < 0.01 vs microwave irradiation group) after genistein pre-incubate. Hoechst 33258 fluorescent staining revealed that the nucleus crimpled, cres-cent liked and chromatin condensed, even nucleus disintegrated. Our research showed that microwave irradiation could lead to large amount of cell apoptosis and necrosis, and genistein pre-treatment could reduce the ratio of apoptosis and necrosis than that in microwave irradiation group (P < 0.01). The concentration of MDA in microwave irradiation group was higher than that in control group (P < 0.01). At the same time, the activity of SOD was significantly reduced (P < 0.01). Pre-incubated with genistein could not decrease the MDA levels, but could increase the activities of SOD (P < 0.01 vs microwave irradiation group). Conclusion: microwave irradiation can induce human proximal renal tubular epithelial cells injury. The protective effects of genistein may partly correlated with decreasing oxidative stress damage and cell apoptosis in HKC cells.
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    The relationship between reserve capacity of microcirculatory blood perfusion and related biochemical indices of male rowers in six weeks' pre-competition training
    ZHANG Hao-nan, GAO Bing-hong, ZHU Huan
    CJAP. 2017, 33 (2): 112-116.   DOI: 10.12047/j.cjap.5429.2017.029
    Abstract   PDF (955KB) ( 61 )
    Objective: To study the change of reserve capacity of microcirculatory blood perfusion (MBP) and the relationship between some conventional function indices of elite male rowers in 6 weeks before the Olympic Games. The feasibility of the MBP using for athletes fu nctional status monitoring was also discussed. Methods: Eight male lightweight rowers of Chinese National Rowing Team were selected as the subjects. Blood samples were taken every Monday morning before eating breakfast (6:30-7:00). The indices including hemoglobin (Hb), red blood cells (RBC),blood urea (BU) and creatine kinase (CK) were tested,while reserve capacity of MBP were collected with PeriFlux 5000 system on the same day. Meanwhile,the venous blood samples were collected and the levels of testosterone (T) and cortisol (C) were deter-mined in the high load training week and last two weeks before the Olympic Games. Results: All presented a certain relevance between the re-serve capacity of MBP and conventional function indices. There was significant positive correlation with T (P < 0.05), and it was correlated with Hb, C and T/C positively, negative correlation with CK, and they all did not have significant difference(P > 0.05), but there was sig-nificant negative correlation with BU (P < 0.05). Conclusion: There is a certain consistency between change of reserve capacity of MBP and the characteristic of conventional function indices. And reserve capacity of MBP is more sensitive in fatigue monitoring after intensity training. In a certain extent, reserve capacity of MBP as a non-invasive indices can be used for evaluating the functional state and judging the degree of fat igue.
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    Effects of dietary corn peptide and exercise on hepatic ATGL and TNF-α in obese rats
    SUN Peng, TIAN Xiang-yang, SHI Reng-fei
    CJAP. 2017, 33 (2): 117-120.   DOI: 10.12047/j.cjap.5416.2017.030
    Abstract   PDF (1181KB) ( 14 )
    Objective: To determine whether dietary corn peptides supplementation with or without exercise on body composition and fac-tors related to hepatic lipid metabolism in obese rats induced by a high fat diet. Methods: Male SD rats were fed a high-fat diet (40% calorie as fat) for 8 weeks as a dietary induced obesity model (DIO, n=32). They were then randomly divided into four groups, control, corn pip-tide(CP) group, exercise group and CP + exercise group (n=8). Supplement (CP:200 g/kg) and exercise(tread mill Ex was performed 15 m/min for 60 min/d, 5 days a week) protocol for 4 weeks. Body weight and dietary levels of rats were monitored during the experimental peri-od. At the end of the 4th weeks, rats of all groups were sacrificed. Perirenal fat and liver were harvested, and hepatic adipose triglyceride li-pase(ATGL) and TNF-α were analyzed via Western blot. Results: After 4 weeks, compared with DIO control group, body weight and perire-nal fat mass index in CP + exercise group were significantly decreased (P < 0.05). Hepatic ATGL was significantly higher in corn peptides supplementation, especially in combination with exercise (P < 0.05). At the same time, the values of TNF-α expression were decreased signif-icantly in exercise groups compared with DIO group (P < 0.05). Conclusion: Corn peptides combined with aerobic exercise has a weight re-duction by lowering adipose tissue in experimental animals, it may be related to the expression of ATGL and TNF-α in the liver, and promote lipid metabolism.
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    The relationship between acute inflammatory cytokines, nerve function defect, daily living ability and PSD
    LI Ping, ZHANG Qiao-lian, LI Shuang-ying
    CJAP. 2017, 33 (2): 121-123.   DOI: 10.12047/j.cjap.5512.2017.031
    Abstract   PDF (935KB) ( 123 )
    Objective: To investigate the correlation between poststroke depression (PSD) and serum levels of inflammatory cytokines, neurologic impairment, daily life ability in patients with acute cerebral infarction at different time. Methods: Two hundreds and eighty patients who admitted to our hospital with a diagnosis of acute infarction excluded the patients mismatch conditions were evaluated by Hamilton depres-sion rating scale (HDRS) to diagnose PSD respectively at admission and 3 months after stroke. Serum inflammatory cytokines high-sensitivity C-reactive protein(hs-CRP), tumor necrosis factor-α(TNF-α) and interleukin-6(IL-6) were determined. NIH stroke scale(NIHSS) and Barthel index for daily life ability were used to evaluate nerve functions. Then we analyzed the correlation between PSD and serum inflammatory cytokines, correlation between PSD and functional impairment and daily life ability at different time. Logistic regression was performed to ana-lyze the risk factors of PSD. Results: The PSD incidence was higher in recovery stage than that in acute stage, but there was no difference. Serum inflammatory cytokines were higher in PSD group at admission than that in non-PSD group. The NIHSS score and Barthel index in PSD group were different from those in non-group at acute and recovery stage. The OR score was 1.765, 1.646, 1.817, 1.188 and 2.015 respec-tively to TNF-α, IL-6 and Barthel index in the acute phase and to NIHSS and Barthel index in recovery stage. Conclusion: The pathogenesis of PSD at different courses of stroke is not same. TNF-α, IL-6 and Barthel index are the independent risk factors of PSD in acute phase, so do NIHSS score and Barthel index in recovery period.
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    Intervention effects of oral active AdipoRon on liver oxidative stress in type 2 diabetic mice
    XIAO Min, QU Xiao-hu, CHEN Hui, CAI Qiang-jun, XIE Ke-jian
    CJAP. 2017, 33 (2): 124-127.   DOI: 10.12047/j.cjap.5451.2017.032
    Abstract   PDF (1482KB) ( 81 )
    Objective: To explore the intervention effects of oral active AdipoRon on liver oxidative stress in type 2 diabetic mice, which provides basic data for clinical application. Methods: Thirty-two healthy male C57BL/6 mice were divided into 4 groups:normal group (NC, n=8), diabetes mellitus group (DM, n=8), high dose AdipoRon treatment group (DM + H, n=8) and low dose AdipoRon treatment group (DM + L, n=8). Following six weeks high fat feed, mice of DM, DM + H and DM + L were intraperitoneally injected with 40 mg/kg streptozocin (STZ), leading to type 2 diabetes. Afterwards, DM + H group and DM + L group were continuously treated with high dose and low doses of oral AdipoRon respectively for 10 days, following which, related biochemical indicators were detected. Western blot method was used to detect the p-IRS-1 protein expression in liver tissue and RT-PCR method to detect PDX-1 mRNA expression in the pancreas. Results: The blood glucose of DM group was obviously higher than that of NC group (P < 0.05). Compared to that of DM group, blood glucose of DM + H group as well as DM + L group was significantly lower. Activity of superoxide dismutase (SOD), catalase (CAT) in liver tissue of DM mice was significantly lower than that of NC group (P < 0.05); activity of malondialdehyde (MDA) and nitric oxide synthase (NOS) in DM group significantly higher than that of NC group (P < 0.05); activity of SOD and CAT in DM + L group and DM + H group obviously higher than DM group (P < 0.05); activity of MDA and NOS in DM + L group and DM + H group significantly lower than DM group (P < 0.05). And the p-IRS-1 protein expression in liver tissue and PDX-1 mRNA level in pancreas increased significantly (P < 0.05). Conclusion: Oral active Adi-poRon which reduced the blood glucose levels of mice had a certain intervention effect on liver tissue oxidative stress in type 2 diabetes mice.
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    The protective effect of exenatide on the renal injury in diabetic rats
    HUANG Qiong-zhu, LIU Xiao-meng, HUANG Guo-quan, WANG Li-juan, ZHENG Yu-jie, CHEN Xin-cheng, LIU Li-bin, CHEN Zhou
    CJAP. 2017, 33 (2): 128-131.   DOI: 10.12047/j.cjap.5466.2017.033
    Abstract   PDF (935KB) ( 75 )
    Objective: To investigate the protective effect of exenatide (Ex) on the renal injury in streptozotocin-induced diabetic rats. Methods: Sprague-Dawley rats were randomly divided into 2 groups:normal control group (NC group, n=8) and model group. Model group was injected with low dose of streptozotocin (30 mg·kg-1) after the rats were fed with high fat and high glucose diet for 4 weeks. Seventy-two hours later, rats of blood glucose level ≥ 16.7 mmol·L-1 were divided into diabetes mellitus group (DM, n=10) and two exenatide-treated groups (Ex groups,3 or 6μg·kg-1, n=8). Ex groups subcutaneously injected with exenatide for 12 weeks, but NC group and DM group were injected with the same volume of solvent. Changes in glycolipid metabolism and renal function such as serum creatinine (Scr), urine creatinine (Ucr), blood urea nitrogen (BUN), 24 hour urine micro-albumin (24 h UMA)in the 4 groups of rats were determined and creatinine clearance rate (Ccr) were calculated. Renal oxidative stress parameters such as superoxide dismutase (SOD), malondialdehyde(MDA), glutathione per-oxidase (GSH-Px) were measured. Hematoxylin-eosin (HE) staining was used to examine pathological morphology in the renal tissues and ELISA was performed to determine the level of advanced glycation end products(AGEs), the glycosylation end product in renal tissues. Results: Compared to the DM group,glycolipid metabolic abnormalities in the exenatide-treated groups were significantly ameliorated with lower levels of blood glucose,HbAlc, cholesterol and triglyceride (P < 0.05). The renal function index was markedly improved (P < 0.05) with Ccr reduced, indicating a high glomerular filtration status. Meanwhile, exenatide treatment improved the diabetes-induced pathological changes in renal morphology, substantially increased the activities of SOD and GSH-Px, and reduced the levels of MDA and AGEs. Conclusion: Exe-natide has the renal protective effect probably by the mechanisms of inhibition of AGEs production and reduction of oxidative stress in the renal tissues of diabetic rats.
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    Protective effects of xuebijing on the acute lung injury in rats
    LUO Peng, ZHOU Zhen-xing
    CJAP. 2017, 33 (2): 132-135.   DOI: 10.12047/j.cjap.5464.2017.034
    Abstract   PDF (936KB) ( 82 )
    Objective: To investigate the protective effects of xuebijing (Traditional Chinese Medicine)on the acute lung injury in rats. Methods: Sixty Wistar rats were randomly divided into normal control group,model group,dexamethasone (10 mg/kg) group, xuebijing low-dose,medium-dose and high-dose groups(5, 10, 15 ml/kg),10 rats in each group. The acute lung injury model was induced by intraperitoneal injection of lipopolysaccharide 5 mg/kg. They were given relevant medicines intraperitoneally after modeling, once a day, for consecutive 7 days. Normal control group and acute lung injury(ALI) model group were given the equal bulk of saline by tail vein. 7 days after treatment, the arterial blood samples were collected for detecting the arterial oxygen tension (PaO2) and the serum levels of malondialdehyde (MDA) and superoxide dismutase (SOD). Pulmonary tissue samples were obtained for the measurement of lung index(LI), wet/dry weight ratio(W/D), moisture content of lung[(W-D)/W] and the expressions of tumor necrosis factor-α (TNF-α), interleukin-10 (IL-10) and high mobility group protein B1(HMGB1). Results: Compared with normal control group,the levels of LI,W/D,(W-D)/W and PaO2 and the expressions of TNF-α, HMGB1 protein and the serum level of MDA in model group were increased,while the protein expression of IL-10 and the serum level of SOD were decreased;there was statistical significance(P < 0.01). Compared with model group,the levels of LI,W/D,moisture content of lung and the protein expressions of TNF-α and HMGB1 and the serum level of MDA in xuebijing groups were decreased significantly,while PaO2 and the protein expression of IL-10 and the serum level of SOD were increased, there was statistical significance (P < 0.01). The effects in xuebi-jing high-dose group were better than those in xuebijing middle-dose and low-dose groups (P < 0.01, P < 0.05), Conclusion: Xuebijing could alleviate lipopolysaccharide-induced ALI,and its pharmacological mechanism may be related to down-regulation the protein expressions of TNF-α and HMGB1 and the serum level of MDA and up-regulation the protein expression of IL-10 and the serum level of SOD and the high-dose is more effective.
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    Preliminary pharmacodynamics study on antiasthmatic action of butylphthalide in guinea pig
    WANG Zhi-wang, WANG Yong-hui, REN Yuan, WANG Rui-qiong, LIN Xing-yao, DUAN Hai-jing
    CJAP. 2017, 33 (2): 136-139.   DOI: 10.12047/j.cjap.5463.2017.035
    Abstract   PDF (1843KB) ( 328 )
    Objective: To study the anti-asthmatic effects of butylphthalide in guinea pig. Methods: This research included isolated tra-cheal smooth muscle and in vivo animal experiments. Antispasmodic effects of butylphthalide at the concentrations of 1, 10, 100 mg/L were observed through spasmodical tracheal smooth muscle of guinea pig induced by acetylcholine or histamine (n=10). After screened, the guinea pigs were divided into control group, model group, dexamethasone(DXM) group, high and low dose butylphthalide groups. The effects of butylphthalide on nitric oxide (NO), endothelin-1 (ET-1) and asthmatic behaviors were observed on the asthmatic guinea pigs that were stimu-lated six times by the excitation fluid (1% ACh:0.05% Hist=1:1). Results: Butylphthalide at the concentrations of 1、10、100 mg/L had an-ti-spasmodic effects on spasmodical tracheal smooth muscle of guinea pig (15.08 ±7.68、42.41 ±13.54、77.56 ±24.82 to acetylcholine, 19.40 ±7.60、56.84 ±11.72、76.35 ±19.40 to histamine), which showed a certain dose-effect relationship. Butylphthalide could prolong asth-matic incubation period (53.3 ±13.2、33.1 ±13.0), improve asthmatic behaviors, reduce NO in serum (78.71 ±19.40、84.75 ±20.97) and ET-1 in bronchoalveolar lavage fluid (24.30 ±5.80、28.50 ±6.31) (P < 0.05, 0.01). Conclusion: Butylphthalide has some effects of anti-asthma and one of the mechanisms is to relieve abnormal increase of NO and ET-1.
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    Establishment and effect observation on three kinds of Ti-Xu with constipation rat models
    DU Li-dong, REN Yuan, NIU Ting-Hui, WU Guo-tai, WANG Zhi-wang, LIU Wu-Zhou, SHAO Jing
    CJAP. 2017, 33 (2): 140-145.   DOI: 10.12047/j.cjap.5479.2017.036
    Abstract   PDF (1267KB) ( 72 )
    Objective: To establish three types constipation models with the syndrome of Xue-Xu, Yin-Xu and Yang-Xu in rats, and to compare the difference of colonic motility, colonic water metabolism, colonic mucus secretion and the level of aquaporin-2 (AQP2). Methods: Forty Sprague Dawley rats, male and female in half, were randomly divided into four groups:normal control group(N), Xue-Xu with constipation group(XC), Yin-Xu with constipation group(YC) and Yang-Xu with constipation group(YAC). Bloodletting and loperamide were used to induce the Xue-Xu with constipation in rats, thyroxin and loperamide were used to induce the Yin-Xu with constipation in rats, ice-water stimulation and loperamide were used to induce the Yang-Xu with constipation in rats. Bloodletting was coducted once a week,drug was ad-ministrated orally to rats once a day for continuous 42 days. Rats'state, body weights, stool character, transfer time between mouth and anal, small intestinal propulsion rate were measured. The colon tissues of rats were stained with alcian blue-periodic acid schiff (AB-PAS) to analyze the changes of mucus secretion. The aquaporin-2 expressions in proximal and distal colon of rats were measured by immunohistochemical method. Results: Compared to normal control group, weight-increasing speed of the rats were reduced in three models. The order of indepen-dent movement change was YC, XC and YAC. Solid stool appeared at 30 d, fecal scores were increased. Transfer time between mouth and anal was significantly extended, small intestinal propulsion rate were significantly decreased(P < 0.05, P < 0.01). Water content in stool were significantly decreased in three type models(P < 0.05, P < 0.01), the content of water in colon were decreased in XC and YAC group (P < 0.05, P < 0.01). The colonic gland and goblet cells were narrowed in some extent, the mucus excretion were decreased. The levels of aquaporin-2 expressions in proximal and distal colon of rats were increased(P < 0.05, P < 0.01), increasing order of AQP2 expression in proximal colon was YAC, YC and XC, increasing order of AQP2 expression in distal colon was YAC, XC and YC. Conclusion: Long term stimulation of complex factors could induce Ti-Xu with constipation animal model, and there were some differences in colonic motility, water metabolism, colonic mucus secretion and AQP2 in rats.
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    Expressions of SERCA2a and miR-25-3p/5p in myocardium of rats with heart failure and therapeutic effects of Xiefei Lishui recipe
    WU Jin-bo, YE Xiao-han, XIAN Shao-xiang, DONG Ming-guo
    CJAP. 2017, 33 (2): 146-150.   DOI: 10.12047/j.cjap.5449.2017.037
    Abstract   PDF (1043KB) ( 122 )
    Objective: To investigate the expressions of sarcoplasmic reticulum calcium ATPase 2a (SERCA2a) mRNA and miR-25-3p/5p in myocardium of rats with heart failure and the therapeutic effects of Xiefei Lishui recipe(Traditional Chinese Medicine). Methods: SD rats were randomly divided into five groups:control group, model group, Traditional Chinese Medicine group(TCM group), captopril group and digoxin group(n=10). Heart failure rat model was induced by intraperitoneal injections of doxorubicin(6 injections within 2 weeks; total dose:15 mg/kg). Five weeks after the first injection, Distilled water(10 ml/(kg·d)), TCM(22 g/(kg·d)), captopril(19 mg/(kg·d)),and digoxin(32μg/(kg·d))were administrated by gastrogavage for 35 days,respectively. Myocardial expressions of SERCA2a mRNA and miR-25-3p/5p were detected;myocardial activities of SERCA2a were measured; cardiac output(CO) and left ventricular ejection fraction(LVEF) and plasma level of B-type natriuretic peptide (BNP) were measured. Results: Myocardial mRNA expression of SERCA2a was significantly down-regulated in rats with heart failure which could be significantly up-regulated by TCM or captopril or digoxin,while expressions of miR-25-3p and miR-25-5p in myocardium were significantly up-regulated which could be significantly down-regulated by TCM or captopril. SERCA2a activity, CO and LVEF in rats with heart failure were decreased significantly which could be significantly increased by TCM,while plasma level of BNP was increased significantly which could be significantly decreased by TCM or captopril or digoxin. CO in rats with heart failure could be signifi-cantly increased by captopril or digoxin. Conclusion: In rats with heart failure, myocardial mRNA expression of SERCA2a was downregulated, expression of miR-25-3p and miR-25-5p in myocardium was upregulated. Xiefei Lishui recipecan upregulate myocardial mRNA expression of SERCA2a, downregulate expression of miR-25-3p and miR-25-5p in myocardium and improve cardiac function.
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    The effects of YHTJF on lung ischemia/reperfusion injury in mice
    SONG Dong, HAO Mao-lin, XIANG Bing-qian, LUO Zi-yin, SHI Lu, Wu Yiming, WANG Wan-tie
    CJAP. 2017, 33 (2): 151-155.   DOI: 10.12047/j.cjap.5450.2017.038
    Abstract   PDF (1397KB) ( 50 )
    Objective: To explore whether yiqi huoxue tongluo jiedu fang (YHTJF, Traditional Chinese Medicine) alleviates the injury during lung ischemia/reperfusion (I/R) in mice through inhibiting oxidative stress or not. Methods: C57BL/6J male mice (n=70) were randomly divided into 7 groups:control (C), carboxyl methyl cellulose-Na(CMC·Na) + normal control (CC), carboxyl methyl cellulose-Na + sham (CS), carboxyl methyl cellulose-Na + I/R (CIR), carboxyl methyl cellulose-Na + YHTJF-Low, CMC-Na + YHTJF-Middle, CMC-Na + YHTJF-High (CYL, CYM, CYH). The mice in CYL, CYM and CYH group were treated with YHTJF by intraperitoneal injection every day, while the carboxyl methyl cellulose-Na was administered with the same volume of CYL in CC, CS and CIR group. After 3 h-reperfusion, the left lung tissues were harvested to determine the lung wet/dry weight (W/D), the total lung water content (TLW), and the index of quantita-tive evaluation for alveolar damage (IQA). Morphological observation and terminal-deoxynucleoitidyl transferase mediated nick end labeling (TUNEL) were applied to evaluate the structural changes and the apoptosis index (AI) of the lung tissues. The expressions superoxide of dis-mutase(SOD), malondialdehyde(MDA) and myeloperoxidase(MPO) in the lung tissues were detected by kits. Results: Compared with group C, the W/D, TLW, IQA, AI, lung tissue structural changes, and the expressions of MDA and MPO in group I/R were increased obviously (P < 0.01), and the expression of SOD was decreased, while there was no significant difference between group CC and CS. Compared with group I/R, the parameters of these experiments in group CYL, CYM, CYH were all decreased, and the expression of SOD was increased, while the reduction in group CYM was the most remarkable among them (P < 0.01). Conclusion: YHTJF may attenuate the I/R injury of the lung by the inhibition of apoptosis via ROS pathway.
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    Effects and mechanism of bFGF on rat myoblast oxidative injury induced by hydrogen peroxide
    MAO Ting-ting, FANG Hong-bo, WANG Xiao-hui, PAN Ying-ying, XIE Hao-huang, ZHANG Hong-yu, XIAO Jian, JIANG Li-ping
    CJAP. 2017, 33 (2): 159-163.   DOI: 10.12047/j.cjap.5446.2017.040
    Abstract   PDF (1181KB) ( 56 )
    Objective: To explore the protective role of basic fibroblast growth factor (bFGF) on attenuating hydrogen peroxide-induced injury in cultured rat myoblasts. Methods: Cultured rat myoblasts at growth phase were randomly divided into four groups (n=6):control group (control), bFGF group (bFGF), model group(H2O2) and the treatment group (bFGF + H2O2). Model group was treated with 100 μmol/L hydrogen peroxide for 4h. B-cell lymphoma-2 (Bcl-2) positive particles were detected by immunohistochemistry; Reactive oxygen species (ROS) and expression for Bcl-2 associated X protein (Bax), Bcl-2 and Cytochrome C (Cyt. C) fluorescence were observed under the invented microscope; Cyt. C and Poly ADP-ribose polymerase(PARP)protein were assessed by Western blot. Results: Compared with control group, the myoblats in the model group showed low expression of Bcl-2 positive particles, accompanied by high expression of ROS level and Cyt. C fuorescence (P < 0.05); Compared with model group, bFGF enhanced Bcl-2 activity of the myoblasts, and significantly downregulated Cyt. C and PARP expression (P < 0.05). Conclusion: bFGF could attenuate oxidative injury of rat myoblasts induced by hydrogen peroxide, which mechanism might be related to enhanced Bcl-2 and reduced ROS, Cyt. C levels.
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    MiR-218 inhibits HTR-8 cells migration and invasion by targeting SOX4
    CHEN Yu-juan, WU Pei-yang, GAO Rui-qiu
    CJAP. 2017, 33 (2): 169-173.   DOI: 10.12047/j.cjap.5453.2017.043
    Abstract   PDF (2803KB) ( 237 )
    Objective: To verify whether miR-218 could inhibit human trophoblastic cell (HTR-8 cells) migration and invasion by target-ing sex determining region Y-box 4(SOX4). Methods: The serum samples were collected from 46 hypertensive disorder complicating pregnan-cy (HDCP) and 50 normal pregnant women. RT-PCR was used to test the expression of miR-218 in the serum. In vitro, MiR-218 was trans-fe cted into HTR-8 cells. The HTR-8 cells were divided into three groups:normal control group, mimic control and miR-218 mimic group. The migratory and invasion ability of HTR-8 cells was tested, and the expressions of matrix metalloproteinase-2(MMP-2), MMP-9 and Sox4 were also investigated in the cells of each group. Luciferase assay was used to confirme whether Sox4-3'-UTR was the target gene of miR-218. Results: The expression of miR-218 was decreased in the serum of HDCP patients compared with the normal pregnant woman(P < 0.01). In vit-ro, compared with the control group, the invasion and migration ability of HTR-8 cells and the expression of MMP-2 MMP-9 and SOX4 were decreased in the miR-218 group (P < 0.01); The Luciferase activity of the SOX4-3'-UTR plasmid was significantly suppressed by miR-218 (P < 0.01); Over expression of SOX4 could reverse the effect of miR-218 on HTR-8 cells(P < 0.01). Conclusion: The expression of miR-218 decreases in the serum of HDPC patients and miR-218 inhibits HTR-8 cells invasion by targeting SOX4-3'-UTR.
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    The research of the progesterone and estrogen secretion level and the FSHR, LHR gene quantitative in granular cells cultured in vitro in different time of laying hens
    LI Yue, JI Hong, XUE Lin-lin, MA Li, YANG Huan-min, LIAN Shuai, GUO Wen-jin, ZHANG YU-chen, ZHAI Jun-fei, GUO Jing-ru, LIU Yan-zhi, ZHEN Li
    CJAP. 2017, 33 (2): 174-178.   DOI: 10.12047/j.cjap.5458.2017.044
    Abstract   PDF (1578KB) ( 102 )
    Objective: To research the hormone secretion levels of progesterone and estrogen and the gene expression levels of two go-nadotropin receptors follicle stimulating hormone receptor (FSHR) and luteinizing hormone receptor (LHR) in granular cells of laying hen, and the effect of culture time on the levels of hormone secretion and expression of related receptor gene in granulosa cells was inferred. Methods: The experiment using the method of cells culture in vitro, the granular cells supernatants of hens were collected at 0 h, 24 h, 48 h, 72 h, 96 h, the progesterone and estrogen concentrations in cell supernatants were determined by ELISA kits, and detected the expression of FSHR and LHR gene in granular cells by real-time fluorescent quantitative PCR. Results: The results showed that the progesterone and estrogen secretion reduced in the early culture of 0 h~48 h(P < 0.05), with the culture time increases to 72 h, the secretion of two hormones began to in-crease, and reaching the level of the initial level of culture. When the cells were cultured to 96 h, the rogesterone and estrogen secretion was reduced again. The lower levels of FSHR and LHR mRNA expression in granular cells appeared with the increase of culture time, compared with the group of cell culture to 0 h, the mRNA expression levels of each groups reduced obviously(P < 0.05). Conclusion: The amount of progesterone and estrogen in the cultured follicular granulosa cells decreased with the increase of in vitro culture time, and then increased. This might be related to the growth state of cells cultured in vitro. But on the whole, with the extension of the training time, the secretion of proges-terone and estrogen in the cells decreased. This may be related to the decreased expression of the FSHR and LHR genes in the two go-nadotropin receptors.
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    The expressions and functions of inflammatory cytokines, growth factors and apoptosis factors in the late stage of pressure ulcer chronic wounds
    Wang Ying, Dai Yai-li, Piao Jin-long, Liu Chun-jie, Li Meng-meng, Jiang Li-ping
    CJAP. 2017, 33 (2): 181-184.   DOI: 10.12047/j.cjap.5425.2017.046
    Abstract   PDF (1462KB) ( 330 )
    Objective: To study the distribution and expressions of inflammatory, growth factors, apoptosis factors in the late stage of pressure ulcer chronic wounds. Methods: Twenty patients were recruited from the First Affiliated Hospital of Henan University during Oc-tomber 2013 to July 2015. Including twenty patients of stage Ⅲ,Ⅳ pressure ulcer, ten acute injury patients and six normal health persons. The histological changes of different wounds were observed by HE staining, the distribution of Caspase-3 protein in four groups was detected by immunohistochemistry technique. The expressions of mRNAs for interleukin (IL)-1β, interleukin (IL)-6, tumor necrosis factor-a (TNF-a), vascular endothelial growth factor (VEGF), vascular endothelial growth factor receptor 2(KDR), fibroblast growth factors(bFGF) and fibrob-last growth factors receptor1 (FGFR1)were determined by real-time reverse transcription polymerase chain reaction. Results: Large numbers of inflammatory cells were found in the stage of Ⅲ, Ⅳpressure ulcer wound under HE staining. The levels of Caspase-3 were mainly localized in fibroblasts or endothelial cells. Compared with the other two groups, the expression of Caspase-3 in pressure ulcers groups was higher (P < 0.01). The expressions of IL-1β, IL-6 and TNF-α were higher than those of the acute group and normal group. The expressions of VEGF, KDR, bFGF and FGFR1 were lower than those of the control group respectively. Conclusion: Overproduction or prolonged expression of in-flammatory factor and apoptosis factor, the expression of VEGF and its receptor KDR and bFGF and its receptor FGFR1 were significantly de-creased in the stage Ⅲ, Ⅳ pressure ulcer wound. These characteristics can be used to comprehensively evaluate etiology and treatment of pressure ulcer chronic wounds.
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    Effect of oxidative stress on myocardial apoptosis, endoplasmic reticulum stress and apoptosis factor in suckling mouse atria myocardium
    LONG Jie, WANG Yu, LIU Xiao-cui, YU Miao, WANG Yi-lin, HUANG Xia, ZHAO Ming
    CJAP. 2017, 33 (2): 185-188.   DOI: 10.12047/j.cjap.5381.2017.047
    Abstract   PDF (1092KB) ( 86 )
    Objective: To investigate the relationship between oxidative stress and myocardial apoptosis, endoplasmic reticulum stress, apoptosis-inducing factors in the process of by researching the effect of oxidative stress on myocardial apoptosis, endoplasmic reticulum stress and apoptosis factor——chop, bax, bcl-2 in suckling mouse atria myocardium. Methods: The primary cultured suckling mouse myocardium were randomly divided into control group and oxidative stress group. Firstly, the suckling mouse atria cardiomyocytes were treated with H2O2 at the concentration of 100 m μmol/L for 2 hours. Then, the index of oxidative stress and anti-oxidative stress superoxide dismutase (SOD), the con-tents of malondialdehyde (MDA) and glutathione (GSH) of this two groups were detected by ELISA. Myocardial apoptosis of the two groups was detected by TUNEL. The expressions of glucose-regulated protein 78 (GRP78), glucose-regulated protein 94 (GRP94), C/EBP homolo-gous protein (chop), Bcl-2 associated X protein (bax), B-cell leukemia 2 protein (bcl-2) mRNA were detected by real time PCR. Results: Compared with the control group, the viability of SOD and the contents of MDA in oxidative stress group were reduced, the contents of MDA was increased (P < 0.01). Compared with the control group, the expression of myocardial apoptosis in oxidative stress group was increased(P < 0.01); the expressions of GRP78, GRP94, chop and bax mRNA were increased, while the expression of bcl-2 mRNA was reduced in ox-idative stress group. Conclusion: Oxidative stress may induce the endoplasmic reticulum stress, activate the expressions of apoptosis factors, and finally increase the myocardial apoptosis of atria cardiomyocytes. This may connected to the incident of atrial fibrillation.
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    Meta-analysis on influence of different exercise intervention modes to blood glucose related indexes of pre-diabetes
    JIANG Ji-quan, XIA Xiao-hui, WANG Hui, ZHANG Zu-hua
    CJAP. 2017, 33 (2): 189-192.   DOI: 10.12047/j.cjap.5400.2017.048
    Abstract   PDF (932KB) ( 15 )
    Objective: To assess the influence of the different exercise intervention mode, aerobic exercise training(AET), resistance training(RT) and AET RT, on blood glucose of pre-diabetes. Methods: Literatures were identified from the PubMed, EMBASE, EBSCO, CNKI and Wanfang databases. Relevant journals or conference proceedings were also searched manually. The qualities of randomized con-trolled trials that conformed to inclusion criteria were evaluated. Meta-analysis of the obtained related data was completed utilizing RevMan 5.2 statistical software. Mean difference of index related in exercise intervention groups and control group were calculated across the studies. Results: Eight original literatures enrolled in this study. Compared with control group, combined effect of different exercise interventions on fast-ing blood glucose showed no significant, consistent with different intervention subgroups analysis. RT group and AET + RT group showed a sig-nificantly reduced role on 2 h postprandial blood index (P < 0.05). AET group showed a significantly reduced role on insulin resistance in-dex. Conclusion: To pre-diabetic, effects of exercise on postprandial 2 h blood glucose and insulin resistance index are related to modes of ex-ercise. Effects of exercise on fasting blood glucose, but still could not be determined.
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