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CJAP ›› 2016, Vol. 32 ›› Issue (2): 106-109.doi: 10.13459/j.cnki.cjap.2016.02.003

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Effects of hypoxia on the survival and Alzheimer's disease-related protein expression in HEK293 cells stably expression APP695 protein

QIAO Meng1, HUANG Xin2, HE Yun-ling2, ZHOU Yan-zhao2, HAN Xue1, ZHU Ling-ling2, FAN Ming1,2   

  1. 1. Department of Neurobiology, Capital Medical University, Beijing 100069;
    2. Department of Cognitive Science, Beijing Institute of Basic Medical Science, Beijing 100850, China
  • Received:2015-04-07 Revised:2015-10-10 Online:2016-03-28 Published:2018-06-12
  • Supported by:
    国家973计划资助项目(2011CB910800,2012CB518200)

Abstract: Objective: To further study the regulation of hypoxia on Alzheimer's disease (AD) pathogenesis, we investigate the effect of hypoxia on the effect of cell survival and expression of related proteins in HEK293 cells stably expressing APP695 Swedish mutantK595N/M596L (HEK293-APP695 cells). Methods: HEK293-APP695 cells were cultured at hypoxia condition (0.3% O2). The survival rate of HEK293-APP695 cells was measured by CCK-8 assay. The protein expression levels of APP, APP-CTFs and BACE1 were detected by Western blot. Results: The survival of HEK293-APP695 cells was obviously decreased after exposed to hypoxia. The expression of APP was reduced, and the expression of APP-CTFs was increased under hypoxia. Conclusion: Our data indicate that hypoxia accelerated cell death of HEK293-APP695 cells by increasing the cleavage of APP and production of β-secretase (β-site amyloid precursor protein cleavage enzyme1, BACE1).

Key words: Alzheimer's disease, hypoxia, HEK293-APP695, APP

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