Molecular of pulmonary arterioles relaxation through SUR2B/Kir6.1 channel opening

doi:10.13459/j.cnki.cjap.2016.03.013

Abstract

Abstract: Objective:To study the dilatation characteristics of ATP-sensitive potassium channel (K_ATP) SUR2B/Kir6.1 subtype opener iptakalim (Ipt) in pulmonary arterioles, and to explore its possible mechanism. Methods:Vessels pressure-diameter monitoring perfusion technique was used to observe the dilatation effects of Ipt in rats fourth pulmonary arterioles (n=6~8). After the pulmonary arterioles were pre-treated with removing endothelium or pre-incubated with K_ATP channel blocker glibenclamide (Gli), cyclo-oxygenase (COX) inhibitor indomethacin (Indo) and nitric oxide synthase (NOS) inhibitor L-Nω-Nitro-arginine methyl ester(L-NAME), the dilatation effects of Ipt were observed. Results:Pulmonary arterioles could be relaxed by Ipt, the maximal relaxation rate was (60.53±2.08)%. Compaired with control group, the effects of Ipt in endothelium denuded arterioles were significantly decreased, the maximal relaxation rate was (9.47±1.56)% (P<0.01). The maximal relaxation rate were decreased to(17.49±1.47)%,(37.00±3.88)% and(24.91±2.30)% respectively after Gli,Indo,L-NAME were pre-incubated (P<0.01). Conclusion:Pulmonary arterioles can be relaxed by Ipt. The selective activation of K_ATP SUR2B/Kir6.1 subtype by Ipt was involved in its mechanisms. The endothelium-dependently dilatation of Ipt was related to nitric oxide (NO) and prostacyclin (PGI₂) released by endothelial cells.

Key words: pulmonary arterioles, Iptakalim, endothelial cells, ATP-sensitive potassium channel, rats

CHEN Jun, WANG Shang, CUI Wen-yu, LONG Chao-liang, ZHANG Yan-fang, ZHANG Hao, WANG Hai. Molecular of pulmonary arterioles relaxation through SUR2B/Kir6.1 channel opening[J]. CJAP, 2016, 32(3): 238-241.

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