Effects of MAPKs inhibitors on GSH metabolic enzymes in rat hepatocytes

doi:10.12047/j.cjap.5601.2017.118

CJAP ›› 2017, Vol. 33 ›› Issue (6): 497-500.doi: 10.12047/j.cjap.5601.2017.118

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Effects of MAPKs inhibitors on GSH metabolic enzymes in rat hepatocytes

GAO Wei-na, PU Ling-ling, WEI Jing-yu, XIN Zhong-hao, WANG Ya-wen, SHI Ta-la, LI Tian, GUO Chang-jiang

Department of Nutrition, Institute of Health and Environmental Medicine, Academy of Military Medical Sciences, Tianjin 300050, China

Received:2016-08-19 Revised:2017-05-21 Online:2017-11-28 Published:2018-06-19
Contact: 蒲玲玲,Tel:022-84655429;E-mail:pulingling@163.com;郭长江,E-mail:guocjtj@126.com E-mail:pulingling@163.com;guocjtj@126.com
Supported by:
国家自然科学基金面上项目（81372988）；天津市应用基础与前沿技术研究计划（青年基金项目，13JCQNJC11800）

Abstract

Abstract: Objective: To investigate the effects of mitogen-activated protein kinases (MAPKs) inhibitors on glutathione (GSH) metabolism, and to explore the pathway related to GSH metabolism.Methods: BRL rat hepatocytes were treated by c-Jun NH2-terminal kinase (JNK),p38, and extracellular signal-regulated kinase 1/2 (ERK1/2) inhibitors:SP600125, SB203580 and PD98659, respectively, for 24 h. MTT method was used to measure hepatocytes viability. The content of GSH was determined by high performance liquid chromatography. The protein expressions of JNK and phosphorylated JNK (p-JNK) was tested by Luminex method. Activities of GSH metabolic enzymes were detected by commercial kits.Results: Hepatocytes vitality was inhibited when the concentrations of SP600125, SB203580 and PD98659 were higher than 10 μmol/L, 20 μmol/L, and 40 μmol/L, respectively; SP600125 decreased the content of GSH in hepatocytes, while SB203580 and PD98659 had no effect. SP600125 reduced p-JNK protein expression, and enhanced GSH-Px activity significantly.Conclusion: JNK MAPK pathway takes part in the GSH metabolism in hepatocytes.

Key words: MAPKs inhibitor, glutathione, metabolism, BRL cell line

GAO Wei-na, PU Ling-ling, WEI Jing-yu, XIN Zhong-hao, WANG Ya-wen, SHI Ta-la, LI Tian, GUO Chang-jiang. Effects of MAPKs inhibitors on GSH metabolic enzymes in rat hepatocytes[J]. CJAP, 2017, 33(6): 497-500.

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Effects of MAPKs inhibitors on GSH metabolic enzymes in rat hepatocytes

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