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CJAP ›› 2016, Vol. 32 ›› Issue (6): 540-544.doi: 10.13459/j.cnki.cjap.2016.06.012

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The Effect of recombinant adiponectin on apoptosis induced by t-BHP in human umbilical vein endothelial cells

WU Li1, LIU Lu-yu1, GUAN Bin-bin1, WANG Lin-xi1,2, LIU Xiao-ying2, CHEN Min1, CHEN Zhou3, LIU Li-bin1,4   

  1. 1. Department of Endocrinology, Fujian Medical University Union Hospital, Fujian Institute of Endocrinology, Fujian Medical University, Fuzhou 350001, China;
    2. Department of Geriatrics, Fujian Medical University Union Hospital, Fujian Medical University, Fuzhou 350001, China;
    3. College of Pharmacy, Fujian Medical University, Fujian Medical University, Fuzhou 350001, China;
    4. Institute of the Elderly Health Sciences, Fujian Medical University, Fuzhou 350001, China
  • Online:2016-11-28 Published:2018-06-19
  • Supported by:
    福建省科技重点项目(2013Y0041);福建省卫生厅青年课题(2009-2-24);国家和福建省临床重点专科建设项目经费资助;福建省卫计委青年项目(2014-1-43);福建医科大学苗圃基金(2014MP031)

Abstract: Objective: To investigate the protective effect and possible mechanism of recombinant adiponectin on apoptosis in Human Umbilical Vein Endothelial Cells (HUVECs) induced by tert-butyl hydroperoxide (t-BHP). Methods: HUVECs were cultured in vitro and apoptosis was induced by t-BHP. On this basis, HUVECs were transfected with adenovirus carrying adiponectin prior to exposure to t-BHP, to further explore the protective effect of adiponectin on apoptosis induced by t-BHP. The percentage of cell viability was determined by MTT assay. The apoptotic rate was evaluated by fluorescence microsopic analysis with Hochest/PI staining. The protein levels of p-JNK, JNK and Caspase 3 were detected by Western blot. Results: Following t-BHP 100 μmol/L administration for 8 h, the ratio of apoptotic cells was increased. Western blot revealed that the protein levels of p-JNK and active caspase 3 were increased(P<0.01) compared to the control group. When cells were pretreated by adenovirus with adiponectin, the apoptosis rate and protein levels of p-JNK and active caspase 3 were decreased significantly(P<0.01). Conclusion: Continuous exposure to t-BHP induced apoptosis in HUVECs. Recombinant adiponectin protected HUVECs from apoptosis induced by t-BHP, which was correlated with the downregulation of p-JNK and active Caspase 3.

Key words: recombinant adiponectin, tert-butylhydroperoxide, oxidative stress, apoptosis, human umbilical vein endothelial cells

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