Improvement effects of applephenon on hypoxia pulmonary hypertension in rats by down-regulating the calcium sensing receptor

doi:10.12047/j.cjap.5591.2018.057

CJAP ›› 2018, Vol. 34 ›› Issue (3): 242-246.doi: 10.12047/j.cjap.5591.2018.057

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Improvement effects of applephenon on hypoxia pulmonary hypertension in rats by down-regulating the calcium sensing receptor

MENG Han-yan¹, ZHAO Jie¹, HUA Chun-yan², ZHU Xing-yu¹, LI Yi-ling¹, YUAN Lin-bo²

1. School of the First Clinical Medical Sciences, Wenzhou Medical University, Wenzhou 325000, China;
2. Department of physiology, School of Basic Medical Sciences, Wenzhou Medical University, Wenzhou 325000, China

Received:2017-04-10 Revised:2018-01-31 Online:2018-05-28 Published:2018-09-08
Supported by:
温州市科技局（Y20170018），浙江省教育厅（Y201223758），浙江省大学生科技创新活动计划暨新苗人才计划（2016R413011），温州医科大学科研课题（wyx201601060），浙江省高等教育教学改革项目（gj20160104），浙江省教育厅教育规划课题（SCG163），温州医科大学高等教育教学研究课题（YBJ201605），地方高校国家级大学生创新创业训练计划（201710343005）

Abstract

Abstract: Objective: To investigate the improving effect of applephenon on the hypoxic-pulmonary hypertension by down-regulating the expression of calcium sensing receptor(CaSR).Methods: Thirty-six male SD rats were randomly divided into 3 groups(n=12):control group, hypoxia-exposure group and applephenon-intervention group.Rats in hypoxia-exposure group and applephenon-intervention group were intermittently exposed to hypoxia in a hypoxic-cabin (the O₂ concentration was 10%) for 8 hours everyday, while rats in control group were raised in normal environment, the experiments were conducted for 4 weeks.Meanwhile, rats in applephenon-intervention group were given intragastrically with applephenon according to 200 mg/kg per day, rats in control and hypoxia-exposure group were given intragastrically with the same amount of normal saline, it lasted for 4 weeks. Then the average pulmonary artery pressure(mPAP), pulmonary vascular resistance(PVR), right ventricular hypertrophy index(RVHI), medial thickness(MT), WA%, WT% and the expression of calcium sensitive receptor (CaSR) of rats in each group were measured.Results: mPAP, PVR, RVHI, MT, WA%, WT% and CaSR expression in hypoxia-exposure group were all significantly higher than those in control group(P<0.01), while applephenon could improved those obviously.Conclusion: Applephenon has a good prophylactic effect on hypoxic pulmonary hypertension, the mechanism may be involved in the down-regulation of CaSR expression.

Key words: applephenon, rats, pulmonary hypertension, calcium sensitive receptor (CaSR)

MENG Han-yan, ZHAO Jie, HUA Chun-yan, ZHU Xing-yu, LI Yi-ling, YUAN Lin-bo. Improvement effects of applephenon on hypoxia pulmonary hypertension in rats by down-regulating the calcium sensing receptor[J]. CJAP, 2018, 34(3): 242-246.

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Improvement effects of applephenon on hypoxia pulmonary hypertension in rats by down-regulating the calcium sensing receptor

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