大黄酸诱导胃癌细胞HGC-27凋亡的作用及其机制*

doi:10.12047/j.cjap.6320.2022.108

中国应用生理学杂志 ›› 2022, Vol. 38 ›› Issue (5): 584-589.doi: 10.12047/j.cjap.6320.2022.108

大黄酸诱导胃癌细胞HGC-27凋亡的作用及其机制^*

陈懿婷⁺, 褚智恒⁺, 傅瑾雯 ,陶嘉颖, 陈佳玉^△

绍兴文理学院医学院, 浙江绍兴 312000

收稿日期:2022-05-12 修回日期:2022-09-28 出版日期:2022-09-28 发布日期:2023-04-23
通讯作者: ^△Tel: 0575-88345826; E-mail: chenyujia10@163.com
作者简介:⁺: 为共同第一作者
基金资助:
^*国家大学生创新创业训练计划项目(202010349038)

Effects of rhein on gastric cancer cells HGC-27 apoptosis and its mechanisms

CHEN Yi-ting⁺, CHU Zhi-heng⁺, FU Jin-wen, TAO Jia-ying, CHEN Jia-yu^△

Shaoxing University School of Medicine, Shaoxing 312000, China

Received:2022-05-12 Revised:2022-09-28 Online:2022-09-28 Published:2023-04-23

摘要/Abstract

摘要： 目的: 探讨大黄酸对胃癌细胞HGC-27增殖和凋亡的影响及其作用机制。方法: 终浓度为0、5、10和20 mg/L的大黄酸在体外分别作用于胃癌HGC-27细胞0、24、48、72 h,每组各设3复孔,使用CCK-8法检测细胞的增殖活力,同时将细胞置于小高内涵显微镜下观察其生长状态,细胞经hoechst染色观察其核型变化,JC-1染色结合流式细胞术分析线粒体膜电位改变,使用流式细胞仪检测细胞周期,RT-qPCR法分析基因的逆转录水平,Western blot分析蛋白表达的改变。结果: 与浓度为0 mg/L大黄酸处理的HGC-27细胞对比,5、10和20 mg/L浓度的大黄酸作用于HGC-27细胞24 h后,细胞线粒体膜电位下降( P＜0.01),细胞增殖活力受到抑制,并诱导其凋亡,作用效果随大黄酸浓度增加和作用时间延长而增强,而细胞周期无显著变化,细胞内bcl-2、jak1、jak2、stat3和notch基因的表达下调,bax、caspase-3基因的表达水平明显升高( P＜0.01)。结论: 大黄酸可通过调节NOTCH /JAK/STAT信号通路来诱导胃癌细胞HGC-27凋亡,具有抗胃癌的效应。

关键词: 胃癌, 大黄酸, 信号通路, 线粒体膜电位, 细胞凋亡, 细胞培养

Abstract: Objective: To investigate the effects of rhein on proliferation and apoptosis of gastric cancer cell line HGC-27 and its related mechanisms. Methods: Human gastric cancer cells HGC-27 were treated with 0, 5, 10 or 20 mg/L rhein respectively for 24, 48 and 72 h in vitro, three duplicate wells were set in each group. The proliferation activity of HGC-27 cells was detected with CCK-8 method, the growth status of HGC-27 cells was observed by small high-content microscope, hoechst staining was used to analyze the karyotype of HGC-27 cells. Mitochondrial membrane potential was detected by JC-1 staining and flow cytometry, cell cycle was analyzed with flow cytometry, the levels of mRNA transcribing of bcl-2, bax, caspase-3, jak1, jak2, stat3 and notch genes were investigated with RT-qPCR method. Protein expressions were determined by Western blot. Results: Compared with HGC-27 cells treated with 0 mg/L rhein, HGC-27 cells treated with 5, 10 and 20 mg/L rhein for 24 h showed decreased mitochondrial membrane potential ( P＜0.01), the cell proliferation activity was inhibited and apoptosis was induced. The effects were enhanced with the increase of rhein concentration and the extension of treatment time, but the cell cycle did not change significantly, and the expressions of bcl-2, jak1, jak2, stat3 and notch genes were down-regulated. The expression levels of bax and caspase-3 genes were increased significantly ( P＜0.01). Conclusion: Rhein can induce apoptosis of HGC-27 cells by influencing NOTCH/JAK/STAT signaling pathway, and has anti-gastric cancer effect.

Key words: gastric cancer, Rhein, signaling pathway, mitochondrial membrane potential, cell apoptosis, cell culture

中图分类号:

陈懿婷, 褚智恒, 傅瑾雯 ,陶嘉颖, 陈佳玉. 大黄酸诱导胃癌细胞HGC-27凋亡的作用及其机制^*[J]. 中国应用生理学杂志, 2022, 38(5): 584-589.

CHEN Yi-ting, CHU Zhi-heng, FU Jin-wen, TAO Jia-ying, CHEN Jia-yu. Effects of rhein on gastric cancer cells HGC-27 apoptosis and its mechanisms[J]. CJAP, 2022, 38(5): 584-589.

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大黄酸诱导胃癌细胞HGC-27凋亡的作用及其机制^*

Effects of rhein on gastric cancer cells HGC-27 apoptosis and its mechanisms

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