氢对高蛋氨酸饮食诱导的高同型半胱氨酸血症和脂肪肝的缓解作用*

doi:10.12047/j.cjap.6382.2022.143

摘要/Abstract

摘要： 目的: 探讨氢(H₂₎对高同型半胱氨酸血症(HHcy)大鼠的同型半胱氨酸(Hcy)水平和非酒精性脂肪肝的缓解作用。方法: Wistar大鼠一周适应性饲养后,随机分为普食组(CHOW)、高蛋氨酸组(HMD)和高蛋氨酸加富氢水组(HMD+HRW)三组,每组8只,CHOW组使用AIN-93G饲料,HMD和HMD+HRW使用AIN-93G+2%蛋氨酸饲料构建HHcy模型,HMD+HRW组同时使用富氢水灌胃(每只3 ml,每天2次,氢浓度为0.8 mmol/L),记录体重数据。饲养6周后处理取材,取大鼠血浆和肝脏,分别检测各组血浆同型半胱氨酸(Hcy)、脂质含量,进行肝脏的组织形态学观察、检测肝脏的Hcy代谢途径关键酶活性和mRNA的表达。结果: 与CHOW组大鼠相比,HMD大鼠血液中Hcy显著升高,差异均具有统计学意义(P＜0.05),病理组织切片观察到大鼠肝肿大,出现损伤并伴有脂肪肝;与HMD组大鼠相比,HMD+HRW组大鼠血液中Hcy明显下降,肝脏损伤减轻,肝脏中Hcy代谢关键酶活性和mRNA表达提高,且差异均具有统计学差异(P＜0.05)。结论: 氢对HMD饮食诱导的HHcy大鼠肝脏损伤有显著的改善作用,可能主要通过增强Hcy三条代谢途径代谢酶来降低体内过量的Hcy,从而改善肝脏代谢功能和非酒精性脂肪肝症状。

关键词: 高同型半胱氨酸血症, 代谢酶, 非酒精性脂肪肝, 富氢水, 叶酸, 大鼠

Abstract: Objective: To investigate the alleviating effect of hydrogen (H₂) on homocysteine (Hcy) levels and non alcoholic fatty liver in rats with hyperhomocysteinemia (HHcy). Methods: After one week of adaptive feeding, Wistar rats were randomly divided into three groups: the general diet group (CHOW), the high methionine group (HMD), and the high methionine plus hydrogen rich water group (HMD+HRW), with 8 rats in each group. The CHOW group was fed with AIN-93G feed, while the HMD and HMD+HRW groups were fed with AIN-93G+2% methionine feed to construct an HHcy model. The HMD+HRW group was also gavaged with hydrogen rich water (3 ml/animal, twice a day, with a hydrogen concentration of 0.8 mmol/L), and body weight data were recorded. After 6 weeks of feeding, the plasma and liver samples were processed and collected. The plasma homocysteine (Hcy) and lipid contents of each group were measured, and the histological morphology of the liver was observed. The activities of key enzymes in the Hcy metabolism pathway and mRNA expression were detected in the liver. Results: Compared with the CHOW group rats, the Hcy level in the blood of HMD rats was significantly increased significantly (P＜0.05). Pathological tissue sections showed liver enlargement, injury, and fatty liver in the rats; Compared with the HMD group rats, the HMD+HRW group rats showed a significant decrease in Hcy in the blood, reduced liver damage, and increased Hcy metabolism key enzyme activity and mRNA expression in the liver, with statistical differences (P＜0.05). Conclusion: Hydrogen has a significant improvement effect on liver injury induced by HMD diet in HHcy rats, possibly by enhancing the three metabolic pathways of Hcy to reduce excessive Hcy in the body, thereby improving liver metabolic function and symptoms of non-alcoholic fatty liver disease.

Key words: hyperhomocysteinemia (HHcy), metabolic enzymes, non-alcoholic fatty liver disease (NAFLD), hydrogen rich water(HRW), folic acid, rats

中图分类号:

R333.4

储文彬, 丁天琪, 文波, 陆俊宇, 樊嵘, 陈学伟. 氢对高蛋氨酸饮食诱导的高同型半胱氨酸血症和脂肪肝的缓解作用^*[J]. 中国应用生理学杂志, 2022, 38(6): 787-792.

CHU Wen-bin, DING Tian-qi, WEN Bo, LU Jun-yu, FAN Rong, CHEN Xue-wei. Alleviating effects of hydrogen on hyperhomocysteinemia and fatty liver induced by high-methionine diet[J]. CJAP, 2022, 38(6): 787-792.

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氢对高蛋氨酸饮食诱导的高同型半胱氨酸血症和脂肪肝的缓解作用^*

Alleviating effects of hydrogen on hyperhomocysteinemia and fatty liver induced by high-methionine diet

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