有氧运动与饮食控制对2型糖尿病大鼠肝chemerin及其受体CMKLR1的影响

doi:10.12047/j.cjap.5495.2017.103

中国应用生理学杂志 ›› 2017, Vol. 33 ›› Issue (5): 426-430.doi: 10.12047/j.cjap.5495.2017.103

有氧运动与饮食控制对2型糖尿病大鼠肝chemerin及其受体CMKLR1的影响

林小晶, 杨宏芳, 王晓慧

上海体育学院运动科学学院, 上海 200438

收稿日期:2016-09-19 修回日期:2017-06-20 出版日期:2017-09-28 发布日期:2018-06-19
通讯作者: 王晓慧,Tel:021-51253520;E-mail:wangpan96@126.com E-mail:wangpan96@126.com
基金资助:
国家自然科学基金资助项目（31271274）

Effects of aerobic exercise and dieting on chemerin and its receptor CMKLR1 in the livers of type 2 diabetic rats

LIN Xiao-jing, YANG Hong-fang, WANG Xiao-hui

School of Kinesiology, Shanghai University of Sport, Shanghai 200438, China

Received:2016-09-19 Revised:2017-06-20 Online:2017-09-28 Published:2018-06-19
Supported by:
国家自然科学基金资助项目（31271274）

摘要/Abstract

摘要： 目的：研究4周有氧运动与饮食控制对2型糖尿病（DM）大鼠肝chemerin及其受体趋化因子样受体1（CMKLR1）的影响及其在改善糖脂代谢中的影响。方法：50只雄性SD大鼠随机分为正常对照组（Con，n=6）和糖尿病造模组（n=44）。采用高脂高糖饲料联合小剂量链脲佐菌素（STZ）（30 mg/kg）的方法制备2型糖尿病模型大鼠。造模成功的DM大鼠随机分为4组（n=6）：糖尿病对照组（DM）、糖尿病运动组（EDM）、改喂普通饲料的糖尿病饮食控制组（NDM）和糖尿病运动+饮食控制组（ENDM）。运动组大鼠进行为期4周中等强度跑台有氧运动，每周运动6 d。采用罗氏血糖仪检测大鼠空腹血糖（FBG），全自动生化分析仪检测大鼠血清总胆固醇（TC）、甘油三酯（TG）和低密度脂蛋白（LDL）水平，real time PCR和Western blot分别检测大鼠肝chemerin、CMKLR1的mRNA和蛋白水平。结果：DM大鼠FBG和血清TC、TG、LDL水平显著升高的同时，肝chemerin和CMKLR1的mRNA和蛋白水平显著增加；4周有氧运动和/或饮食控制显著降低EDM、NDM和ENDM组FBG和血清TC、TG、LDL的同时，显著降低这3组大鼠的肝chemerin蛋白水平，其中ENDM组降低最显著（P<0.01）；NDM和ENDM组大鼠的肝CMKLR1蛋白水平升高（P<0.01）。结论：4周有氧运动和/或饮食控制降低2型糖尿病大鼠的肝chemerin蛋白水平、增加CMKLR1蛋白水平，这可能与其改善糖尿病大鼠的糖脂代谢有关。

关键词: chemerin, CMKLR1, 2型糖尿病, 大鼠, 有氧运动, 饮食控制

Abstract: Objective: To investigate the effects of aerobic exercise and dieting on chemerin and its receptor chemokine like receptor-1 (CMKLR1) in the livers of type 2 diabetes mellitus (DM) rats and its possible roles on the improvements of blood glucose and lipid metabolism.Methods: Fifty male SD rats were randomly divided into control group (Con, n=6) fed with normal diet and DM model group (n=44) fed with high fat diet. After 8-week feeding, DM model rats were intraperitoneal injected streptozocin (STZ) at the dose of 30 mg/kg body weight to establish DM rats. The successfully established diabetes rats were randomly divided into 4 groups (n=6):diabetes control group (DM), diabetes exercise group (EDM), diabetes dieting group by feeding normal diet (NDM) and diabetes exercise plus dieting group (ENDM). During the following 4 weeks, exercise rats participated in moderate intensity aerobic exercise on a treadmill with a progressing increasing load for 4 weeks. Fasting blood glucose (FBG) was determined by Roche glucometer. Serum levels of triglyceride (TG), total cholesterol (TC) and low-density lipoprotein (LDL)) were detected by using full automatic biochemical analyzer. The mRNA and protein expressions of chemerin and CMKLR1 in the livers were detected by real time PCR and Western blot, respectively.Results: Accompanied with the increased serum levels of TC, TG, LDL and FBG in DM rats, the mRNA and protein levels of chemerinand CMKLR1 in the livers of DM rats were significant up-regulated. The serum levels of TC, TG, LDL and FBG were reduced in EDM, NDM and ENDM rats compared with those of DM rats, meanwhile,decreased protein levels of chemerin were found in the livers of EDM, NDM and ENDM rats with most significant in ENDM rats while increased protein levels of CMKLR1 in the livers of NDM and ENDM rats.Conclusion: Four weeks of aerobic exercise with or without dieting decreased chemerin and increased CMKLR1 in the livers of DM rats at the protein levels, which might be attributed to the improvement of blood glucose and lipid metabolism of DM rats.

Key words: chemerin, CMKLR1, type 2 diabetes, rat, aerobic exercise, diet

林小晶, 杨宏芳, 王晓慧. 有氧运动与饮食控制对2型糖尿病大鼠肝chemerin及其受体CMKLR1的影响[J]. 中国应用生理学杂志, 2017, 33(5): 426-430.

LIN Xiao-jing, YANG Hong-fang, WANG Xiao-hui. Effects of aerobic exercise and dieting on chemerin and its receptor CMKLR1 in the livers of type 2 diabetic rats[J]. CJAP, 2017, 33(5): 426-430.

参考文献

[1] Lumeng CN, Saltiel AR. Inflammatory links between obesity and metabolic disease[J]. J Clin Invest, 2011, 121(6):2111-2117.
[2] Weigert J, Neumeier M, Wanninger J, et al. Systemic chemerin is related to inflammation rather than obesity in type 2 diabetes[J]. Clin Endocrinol (Oxf), 2010, 72(3):342-348.
[3] Ernst MC, Sinal CJ. Chemerin:at the crossroads of inflammation and obesity[J]. Trends Endocrinol Metab, 2010, 21(11):660-667.
[4] Li Y, Shi B, Li S. Association between serum chemerin concentrations and clinical indices in obesity or metabolic syndrome:a meta-analysis[J]. PloS One, 2014, 9(12):e113915.
[5] Han J, Kim SH, Suh YJ, et al. Serum Chemerin Levels Are Associated with Abdominal VisceralFat in Type 2 Diabetes[J]. J Korean Med Sci, 2016, 31(6):924-931.
[6] Ernst MC, Issa M, Goralski KB, et al. Chemerin exacerbates glucose intolerance in mouse models of obesity and diabetes[J]. Endocrinol, 2010, 151(5):1998-2007.
[7] Lee MK, Chu SH, Lee DC, et al. The association between chemerin and homeostasis assessment of insulin resistance at baseline and after weight reduction via lifestyle modifications in young obese adults[J]. Clin Chim Acta, 2013, 421:109-115.
[8] Kim SH, Lee SH, Ahn KY, et al. Effect of lifestyle modification on serum chemerin concentrationand its association with insulin sensitivity in overweight and obese adults with type 2 diabetes[J]. Clin Endocrinol (Oxf), 2014, 80(6):825-833.
[9] Chakaroun R, Raschpichler M, Kloting N, et al. Effects of weight loss and exercise on chemerin serum concentrations and adipose tissue expression in human obesity[J]. Metabolism, 2012, 61(5):706-714.
[10] Stefanov T, Bluher M, Vekova A, et al. Circulating chemerin decreases in response to a combined strength and endurance training[J]. Endocrine, 2014, 45(3):382-391.
[11] Neuparth MJ, Proenca JB, Santos-Silva A, et al. The positive effect of moderate walking exercise on chemerin levels in Portuguese patients with type 2 diabetes mellitus[J]. J Investig Med, 2014, 62(2):350-353.
[12] Malin SK, Navaneethan SD, Mulya A, et al. Exercise-induced lowering of chemerin is associated with reduced cardiometabolic risk and glucose-stimulated insulin secretion in older adults[J]. J Nutr Health Aging, 2014, 18(6):608-615.
[13] Chakaroun R, Raschpichler M, Kloting N, et al. Effe cts of weight loss and exercise on chemerin serum concentrations and adipose tissue expression in human obesity[J]. Metabolism, 2012, 61(5):706-714.
[14] Ress C, Tschoner A, Engl J, et al. Effect of bariatric surg ery on circulating chemerin levels[J]. Eur J Clin Invest, 2010, 40(3):277-280.
[15] Schultz S, Beck-Sickinger AG. Chemerin and vaspin:possible targ ets to treat obesity[J]. Chem Med Chem, 2013, 8(4):549-559.
[16] Ernst MC, Haidl ID, Zuniga LA, et al. Disruption of the Che mokine-Like Rec eptor-1(CMKLR1) Gene Is Associated with Reduced Adiposity and Glucose Intolera nce[J]. Endocrinology, 2012, 153(2):672-682.
[17] Huang C, Wang M, Ren L, et al. CMKLR1 deficiency influences glucose tolera nce and thermogenesis in mice on high fat diet[J]. Biochem Biophys Res Comm un, 2016, 473(2):435-441.
[18] Wargent ET, Zaibi MS, O'Dowd JF, et al. Evidence from studi es in rodents and in isolated adipocytes that agonists of the chemerin receptor CMKLR1 may be beneficial in the treatment of type 2 diabetes[J]. PeerJ, 2015, 3:e753.
[19] Bondue B, De Henau O, Luangsay S, et al. The chemerin/ChemR 23 system does not affect the pro-inflammatory response of mouse and human macrophages ex vivo[J]. PloS One, 2012, 7(6):e40043.

有氧运动与饮食控制对2型糖尿病大鼠肝chemerin及其受体CMKLR1的影响

Effects of aerobic exercise and dieting on chemerin and its receptor CMKLR1 in the livers of type 2 diabetic rats

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