葛根素对人非小细胞肺癌A549细胞凋亡的作用

doi:10.12047/j.cjap.5535.2017.111

中国应用生理学杂志 ›› 2017, Vol. 33 ›› Issue (5): 466-469.doi: 10.12047/j.cjap.5535.2017.111

葛根素对人非小细胞肺癌A549细胞凋亡的作用

金鑫¹, 孙盼盼², 洪云³, 虞乐⁴, 李曙⁴

1. 皖南医学院药理学教研室, 芜湖 241000;
2. 皖南医学院护理学教研室, 芜湖 241000;
3. 皖南医学院弋矶山医院超声医学科, 芜湖 241000;
4. 皖南医学院病理生理学教研室, 芜湖 241000

收稿日期:2016-12-09 修回日期:2017-07-03 出版日期:2017-09-28 发布日期:2018-06-19
通讯作者: 李曙,Tel:13965183595;E-mail:258104975@qq.com E-mail:258104975@qq.com
基金资助:
皖南医学院校级重点科研项目培育基金（WK2017Z04）

Puerarin induces apoptosis in A549 cells

JIN Xin¹, SUN Pan-pan², HONG Yun³, YU Le⁴, LI Shu⁴

1. Department of Pharmacology, Wannan Medical College, Wuhu 241002, China;
2. Department of Nursing, Wannan Medical College, Wuhu 241002, China;
3. Department of Ultrasonography, Yijishan Hospital, Wuhu 241002, China;
4. Department of Pathophysiology, Wannan Medical College, Wuhu 241002, China

Received:2016-12-09 Revised:2017-07-03 Online:2017-09-28 Published:2018-06-19
Supported by:
皖南医学院校级重点科研项目培育基金（WK2017Z04）

摘要/Abstract

摘要： 目的：观察葛根素对人非小细胞肺癌A549细胞生长的抑制作用及其机制。方法：体外培养人非小细胞肺癌细胞（A549），不同浓度（60 μg/ml，120 μg/ml，240 μg/ml）葛根素处理24 h后；采用CCK-8法观察葛根素对细胞的增值抑制作用；吖啶橙（AO）/溴化乙锭（EB）双染法及AnnexinⅤ-PI双染流式细胞术检测药物作用前后A549细胞的形态学变化及凋亡状况；Western blot法检测Apelin/APJ蛋白水平的变化。结果：CCK-8法检测结果说明葛根素能抑制A549细胞的增值，具有浓度和时间依赖关系；流式细胞术进一步证实葛根素具有诱导细胞凋亡的作用，与A549细胞组比较，葛根素各处理组Apelin/APJ蛋白水平均有不同程度下调。结论：葛根素可能通过调节Apelin/APJ蛋白的表达诱导A549细胞凋亡。

关键词: 葛根素, A549, Apelin/APJ, 凋亡

Abstract: Objective: To explore the effect of puerarin induced apoptosis in human non-small cell lung canner A549 cells and its possible mechanisms.Methods: A549 cells were cultivated in vitro. When 80%~90% confluence was reached, they were treated with puerarin in different concentration (60、120、240 μg/ml). The effects on cell apoptpsis were examined by CCK-8 colorimetry. Characteristic morphological changes of apoptosis were observed by AO/EB staining. The apoptosis of A549 cells were detected by flow cytometry analysis. The proteins level of Apelin and APJ were tested by Western blot.Results: CCK-8 test showed that puerarin could inhibit the proliferation of A549 cells in a time and concentration dependent manner. Flow cytometry analysis indicated that puerarin could make A549 cells apoptosis. Compared with the A549 cells group, the protein expression of Apelin/APJ was decreased in puerarin groups.Conclusion: Puerarin induces apoptosis in A549 cells, and its mechanism may through the regulation of Apelin/APJ expression.

Key words: puerarin, A-549, Apelin/APJ, apoptosis

金鑫, 孙盼盼, 洪云, 虞乐, 李曙. 葛根素对人非小细胞肺癌A549细胞凋亡的作用[J]. 中国应用生理学杂志, 2017, 33(5): 466-469.

JIN Xin, SUN Pan-pan, HONG Yun, YU Le, LI Shu. Puerarin induces apoptosis in A549 cells[J]. CJAP, 2017, 33(5): 466-469.

参考文献

[1] Yang L, Ling Y, Guo L, et al. Detection of ALK translocation in non-small cell lung carcinoma (NSCLC) and its clinicopathological significance using the Ventana immunohistochemical staining method:a single-center large-scale investigation of 1, 504 Chinese Han patients[J]. Chin J Cancer Res, 2016, 28(5):495-502.
[2] Cheng Y, Leng W, Zhang J. Protective Effect of Puerarin Against Oxidative Stress Injury of Neural Cells and Related Mechanisms[J]. Med Sci Monit, 2016, 22:1244-1249.
[3] Ai F, Chen M, Yu B, et al. Puerarin accelerate scardiac angiogenesis and improves cardiac function of myocardial infarction by upregulating VEGFA, Ang-1 and Ang-2 in rats[J]. Int J Clin Exp Med, 2015, 8(11):20821-20828.
[4] 魏述永. 葛根素心血管保护作用及其机制研究进展[J]. 中国中药杂志, 2015, 40(12):2278-2284.
[5] Wang Y, Ma Y, Zheng Y, et al. In vitro and in vivo anticancer activity of a novel puerarin nanosuspension against colon cancer, with high efficacy and low toxicity[J]. Int J Pharm, 2013, 441(1-2):728-735.
[6] Guo XF, Yang ZR, Wang J, et al. Synergistic antitumor effect of puerarin combined with 5-fluorouracil on gastric carcinoma[J]. Mol Med Rep, 2015, 11(4):2562-2568.
[7] Chen YY, Chen J, Zhou XM, et al. Puerarin protects human umbilical vein endothelial cells against high glucose-induced apoptosis by upregulating heme oxygenase-1 and inhibiting calpain activation[J]. Fundam Clin Pharmacol, 2012, 26(3):322-331.
[8] Xu X, Wang J, Zhang H, et al. Puerarin reduces apoptosis in rat hippocampal neurons culturea in high glucose medium by modulating the p38 mitogen activated protein kinase and c-Jun N-terminal kinase signaling pathways[J]. J Tradit Chin Med, 2016, 36(1):78-84.
[9] Tatemoto K, Hosoya M, Habata Y, et al. Isolation and characterization of a novel endogenous peptide ligand for the human APJ receptor[J]. Biochem Biophys Res Commun, 1998, 251(2):471-476.
[10] McLean DL, Kim J, Kang Y, et al. Apelin/APJ signaling is a critical regulator of statin effects in vascular endothelial cells——brief report[J]. Arterioscler Thromb Vasc Biol, 2012, 32(11):2640-2643.
[11] Zeng XX, Wilm TP, Sepich DS, et al. Apelin and its receptor control heart field formation during zebrafish gastrulation[J]. Dev Cell, 2007, 12(3):391-402.
[12] Hamada J, Baasanjav A, Ono N, et al. Possible involvement of downregulation of the apelin-APJ system in doxorubicin-induced cardiotoxicity[J]. Am J Physiol Heart Circ Physiol, 2015, 308(8):H931-941.
[13] Sorli S C, Le Gonidec S, Knibiehler B, et al. Apelin is a potent activator of tumour neoangiogenesis[J]. Oncogene, 2007, 26(55):7692-7699.
[14] Berta J, Kenessey I, Dobos J, et al. Apelin Expression in Human Non-small Cell Lung Cancer:Role in Angiogenesis and Prognosis[J]. J Thorac Oncol, 2010, 5(8):1120-1129.
[15] Wang Z, Greeley GH, Jr Qiu S. Immunohistochemical localization of apelin in human normal breast and breast carcinoma[J]. J Mol Histol, 2008, 39(1):121-124.

葛根素对人非小细胞肺癌A549细胞凋亡的作用

Puerarin induces apoptosis in A549 cells

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