首页  期刊介绍 征稿简则 编委会 期刊征订 广告服务 留言板 联系我们 English

中国应用生理学杂志 ›› 2017, Vol. 33 ›› Issue (5): 466-469.doi: 10.12047/j.cjap.5535.2017.111

• 研究论文 • 上一篇    下一篇

葛根素对人非小细胞肺癌A549细胞凋亡的作用

金鑫1, 孙盼盼2, 洪云3, 虞乐4, 李曙4   

  1. 1. 皖南医学院药理学教研室, 芜湖 241000;
    2. 皖南医学院护理学教研室, 芜湖 241000;
    3. 皖南医学院弋矶山医院超声医学科, 芜湖 241000;
    4. 皖南医学院病理生理学教研室, 芜湖 241000
  • 收稿日期:2016-12-09 修回日期:2017-07-03 出版日期:2017-09-28 发布日期:2018-06-19
  • 通讯作者: 李曙,Tel:13965183595;E-mail:258104975@qq.com E-mail:258104975@qq.com
  • 基金资助:
    皖南医学院校级重点科研项目培育基金(WK2017Z04)

Puerarin induces apoptosis in A549 cells

JIN Xin1, SUN Pan-pan2, HONG Yun3, YU Le4, LI Shu4   

  1. 1. Department of Pharmacology, Wannan Medical College, Wuhu 241002, China;
    2. Department of Nursing, Wannan Medical College, Wuhu 241002, China;
    3. Department of Ultrasonography, Yijishan Hospital, Wuhu 241002, China;
    4. Department of Pathophysiology, Wannan Medical College, Wuhu 241002, China
  • Received:2016-12-09 Revised:2017-07-03 Online:2017-09-28 Published:2018-06-19
  • Supported by:
    皖南医学院校级重点科研项目培育基金(WK2017Z04)

摘要: 目的:观察葛根素对人非小细胞肺癌A549细胞生长的抑制作用及其机制。方法:体外培养人非小细胞肺癌细胞(A549),不同浓度(60 μg/ml,120 μg/ml,240 μg/ml)葛根素处理24 h后;采用CCK-8法观察葛根素对细胞的增值抑制作用;吖啶橙(AO)/溴化乙锭(EB)双染法及AnnexinⅤ-PI双染流式细胞术检测药物作用前后A549细胞的形态学变化及凋亡状况;Western blot法检测Apelin/APJ蛋白水平的变化。结果:CCK-8法检测结果说明葛根素能抑制A549细胞的增值,具有浓度和时间依赖关系;流式细胞术进一步证实葛根素具有诱导细胞凋亡的作用,与A549细胞组比较,葛根素各处理组Apelin/APJ蛋白水平均有不同程度下调。结论:葛根素可能通过调节Apelin/APJ蛋白的表达诱导A549细胞凋亡。

关键词: 葛根素, A549, Apelin/APJ, 凋亡

Abstract: Objective: To explore the effect of puerarin induced apoptosis in human non-small cell lung canner A549 cells and its possible mechanisms.Methods: A549 cells were cultivated in vitro. When 80%~90% confluence was reached, they were treated with puerarin in different concentration (60、120、240 μg/ml). The effects on cell apoptpsis were examined by CCK-8 colorimetry. Characteristic morphological changes of apoptosis were observed by AO/EB staining. The apoptosis of A549 cells were detected by flow cytometry analysis. The proteins level of Apelin and APJ were tested by Western blot.Results: CCK-8 test showed that puerarin could inhibit the proliferation of A549 cells in a time and concentration dependent manner. Flow cytometry analysis indicated that puerarin could make A549 cells apoptosis. Compared with the A549 cells group, the protein expression of Apelin/APJ was decreased in puerarin groups.Conclusion: Puerarin induces apoptosis in A549 cells, and its mechanism may through the regulation of Apelin/APJ expression.

Key words: puerarin, A-549, Apelin/APJ, apoptosis

版权所有 © 2015 《中国应用生理学杂志》编辑部
京ICP备16058274号-1
地址:天津市和平区大理道1号,邮编:300050  电话:022-23909086  E-mail:editor@cjap.ac.cn
本系统由北京玛格泰克科技发展有限公司设计开发 技术支持:support@magtech.com.cn