氢气对慢性间歇性低氧大鼠肝脏氧化应激损伤的改善作用

doi:10.12047/j.cjap.5484.2018.016

中国应用生理学杂志 ›› 2018, Vol. 34 ›› Issue (1): 61-64.doi: 10.12047/j.cjap.5484.2018.016

氢气对慢性间歇性低氧大鼠肝脏氧化应激损伤的改善作用

杨胜昌¹, 陈玲玲², 傅天¹, 李文雅¹, 吉恩生¹

1. 河北中医学院, 石家庄 050200;
2. 河北医科大学, 石家庄 050017

收稿日期:2016-11-04 修回日期:2017-06-21 出版日期:2018-01-28 发布日期:2018-06-19
通讯作者: 吉恩生,Tel:0311-89926098;E-mail:jesphy@126.com E-mail:jesphy@126.com
基金资助:
河北省教育厅高等学校科学技术重点研究项目资助（ZD2014043）；河北省高等学校自然科学研究青年基金项目（QN2016166）

Improvement of hydrogen on liver oxidative stress injury in chronic intermittent hypoxia rats

YANG Sheng-chang¹, CHEN Ling-ling², FU Tian¹, LI Wen-ya¹, JI En-sheng¹

1. Department of Physiology, Hebei University of Chinese Medicine, Shijiazhuang 050200;
2. Department of Physiology, Hebei Medical University, Shijiazhuang 050017, China

Received:2016-11-04 Revised:2017-06-21 Online:2018-01-28 Published:2018-06-19
Supported by:
河北省教育厅高等学校科学技术重点研究项目资助（ZD2014043）；河北省高等学校自然科学研究青年基金项目（QN2016166）

摘要/Abstract

摘要： 目的：研究氢气对慢性间歇性低氧大鼠肝脏损伤的改善作用。方法：24只雄性成年SD大鼠，随机分为3组（n=8）：常氧组（Norm）、慢性间歇性低氧组（CIH）、氢气+慢性间歇性低氧组（H₂+CIH）。Norm组暴露于空气中，CIH组与H₂+CIH组接受间歇性低氧处理5周，其中H₂+CIH组在间歇性低氧处理前给予1 h 67%浓度的氢气吸入。5周后比较各组大鼠血清氧化应激指标、炎症因子指标、肝酶水平、血脂水平，并在电镜下观察大鼠肝组织超微结构变化。结果：与Norm组相比，CIH组肝组织超微结构受损严重，谷丙转氨酶（ALT）、谷草转氨酶（AST）水平显著升高（P<0.05）；血清8-羟基脱氧鸟苷（8-OHdG）水平显著升高；超氧化物歧化酶（SOD）活性显著降低；白介素-6（IL-6）水平显著升高。与CIH组相比，H₂+CIH组肝组织超微结构损伤减轻，ALT、AST水平显著降低（P<0.05）；8-OHdG与IL-6水平显著降低，SOD活性显著升高。与Norm组相比，CIH组IL-1水平升高；血清TC、TG、LDL水平升高，但无统计学差异。HDL在各组之间无统计学差异。结论：氢气可以减轻慢性间歇性低氧对大鼠肝脏的损伤，有效降低氧化应激水平，保护肝细胞受损。

关键词: 氢气, 间歇性低氧, 肝脏, 氧化应激, 大鼠

Abstract: Objective: To explore the effects of hydrogen on liver injury in chronic intermittent hypoxia rats and the related oxidative stress mechanism.Methods: Twenty-four male adult SD rats were randomly divided into 3 groups(n=8):the normoxia group (Norm), the chronic intermittent hypoxia group (CIH), the chronic intermittent hypoxia and hydrogen group (H₂ + CIH). Rats in Norm group were exposed in air, those in the other 2 groups suffered from chronic intermittent hypoxia conditions for 5 weeks. Before the CIH treatment, rats in H₂+CIH group inhaled hydrogen gas at 67% concentration for 1 hour. The serum biochemical indicators of oxidative stress, pro-inflammatory cytokine, liver enzyme and blood lipid were inspected after five weeks treatment, the pathological changes of liver tissue were also observed in the transmission electron microscope.Results: Compared with Norm group, the microstructure of liver cells was severely injured, and the serum levels of glutamic-pyruvic transaminase(ALT),glutamic-oxalacetic transaminase (AST) were significantly higher in CIH group (P<0.05); the serum level of 8-hydroxy-2 deoxyguanosine(8-OHdG) and interleukin-6(IL-6) was significantly higher, the serum level of superoxide dismutase (SOD) was significantly lower. Compared with CIH group, the pathology of liver microstructure were significantly improved and the serum levels of ALT, AST were significantly lower in H₂+CIH group (P<0.05); the serum levels of 8-OHdG and IL-6 were significantly lower, the serum level of SOD was significantly higher. Compared with Norm group, the serum level of IL-1 was higher, the serum level of TC, TG, and low density lipoprotein(LDL) were lower, but there was no statistical difference with those in CIH group. There was no statistical difference in the serum level of high density lipoprotein (HDL)among the three groups.Conclusion: Pre-treatment with hydrogen could improve the liver injury caused by chronic intermittent hypoxia, and reducing oxidative stress level for protecting the liver cells damage.

Key words: hydrogen, chronic intermittent hypoxia, liver, oxidative stress, rat

杨胜昌, 陈玲玲, 傅天, 李文雅, 吉恩生. 氢气对慢性间歇性低氧大鼠肝脏氧化应激损伤的改善作用[J]. 中国应用生理学杂志, 2018, 34(1): 61-64.

YANG Sheng-chang, CHEN Ling-ling, FU Tian, LI Wen-ya, JI En-sheng. Improvement of hydrogen on liver oxidative stress injury in chronic intermittent hypoxia rats[J]. CJAP, 2018, 34(1): 61-64.

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氢气对慢性间歇性低氧大鼠肝脏氧化应激损伤的改善作用

Improvement of hydrogen on liver oxidative stress injury in chronic intermittent hypoxia rats

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