一次性力竭运动过程中大鼠“黑质-丘脑-皮层”通路神经元相干性及递质动态变化

doi:10.12047/j.cjap.5435.2017.051

中国应用生理学杂志 ›› 2017, Vol. 33 ›› Issue (3): 204-211.doi: 10.12047/j.cjap.5435.2017.051

一次性力竭运动过程中大鼠“黑质-丘脑-皮层”通路神经元相干性及递质动态变化

胡琰茹^1,2, 刘晓莉¹, 乔德才¹

1. 北京师范大学体育与运动学院, 北京 100875;
2. 中南大学体育教研部, 湖南长沙 410083

收稿日期:2016-03-07 修回日期:2016-03-07 出版日期:2017-05-28 发布日期:2018-06-20
通讯作者: 乔德才,Tel:010-58802227,E-mail:decaiq@bnu.edu.cn E-mail:decaiq@bnu.edu.cn
基金资助:
国家自然科学基金资助项目（31171138，31571221）；湖南省体育科学学会重点资助项目（2015HN016）

Dynamic changes of ‘substantianigra-ventralislateralis-cortex’ pathway neural activity coherence and neurotransmitters in rat during exhausting exercise

HU Yan-ru^1,2, LIU Xiao-li¹, QIAO De-cai¹

1. College of Physical Education and Sports, Beijing Normal University, Beijing 100875;
2. Department of Physical Education, Central South University, Changsha 410083, China

Received:2016-03-07 Revised:2016-03-07 Online:2017-05-28 Published:2018-06-20
Supported by:
国家自然科学基金资助项目（31171138，31571221）；湖南省体育科学学会重点资助项目（2015HN016）

摘要/Abstract

摘要： 目的：通过观察一次性力竭运动过程中大鼠"黑质-丘脑-皮层"通路核团间神经元电活动的相干性及各核团神经递质谷氨酸（Glu）和γ-氨基丁酸（GABA）的浓度，探讨运动疲劳发生过程中大鼠"黑质-丘脑-皮层"通路神经元电活动的可能机制。方法：40只雄性Wistar大鼠，随机分为神经元电活动实时测定组、黑质网状部（SNr）神经元胞外神经递质实时测定组、丘脑腹外侧核（VL）神经元胞外神经递质实时测定组及皮层辅助运动区（SMA）神经元胞外神经递质实时测定组（n=10）。大鼠提供3级递增负荷跑台运动方案进行一次性力竭运动，通过自身对照，观察神经元电活动实时测定组大鼠SNr、VL及SMA神经元细胞在一次性力竭运动前、中、后神经元电活动的动态变化，同步、动态观察神经元胞外神经递质实时测定组大鼠一次性力竭运动前、中、后大鼠SNr、VL及SMA胞外Glu和GABA的浓度及其比值变化。结果：黑质网状部、丘脑腹外侧核局部场电与皮层脑电在力竭运动过程的不同阶段脑区之间神经元电活动，在0~30 Hz范围内均存在显著相干性。与安静状态相比较，自主运动期，大鼠黑质网状部神经元胞外Glu浓度、Glu/GABA比值均显著下降（P < 0.05，P < 0.01），GABA浓度则显著升高（P < 0.05，P < 0.01），而丘脑腹外侧核及皮层辅助运动区神经元胞外Glu浓度、Glu/GABA比值均显著升高（P < 0.05，P < 0.01），GABA浓度则显著下降（P < 0.05，P < 0.01）；疲劳初期和力竭期，大鼠黑质网状部神经元胞外Glu浓度、Glu/GABA比值均显著升高（P < 0.05，P < 0.01），GABA浓度则显著下降（P < 0.05，P < 0.01），而丘脑腹外侧核及皮层辅助运动区神经元胞外Glu浓度、Glu/GABA比值均显著下降（P < 0.05，P < 0.01），GABA浓度则显著升高（P < 0.05，P <0.01）。结论：大鼠在一次性力竭运动过程中"黑质-丘脑-皮层"通路各核团之间存在神经网络联系，该通路各核团神经递质Glu、GABA浓度的改变是导致其神经元电活动变化的因素之一。

关键词: 力竭运动, 大鼠, “黑质-丘脑-皮层”通路, 相干性, 神经递质

Abstract: Objective: To reveal the possible mechanism of changes of ‘substantianigra-ventralislateralis-cortex’ pathway neural activity during one bout of exhausting exercise through observing the neural activity coherence between different nucleus and the concentration of extra-cellular glutamate (Glu) and gamma-aminobutyric acid (GABA). Methods: Male Wistar rats were randomly divided into neural activity real-time observation group, substantianigra (SNr) extracellular neurotransmitters observation group, ventralislateralis (VL) extracellular neuro-transmitters observation group and supplementary motor area (SMA) extracellular neurotransmitters observation group, 10 rats in each group. For rats of neural activity real-time observation group, by using LFPs and ECoG recording technique, and self-comparison, we simultaneously recorded the dynamic changes of neural activity of rat SNr, VL and SMA during one bout of exhausting exercise. The dynamic changes of ex-tracellular Glu and GABA in rat SNr, VL and SMA were also observed through microdialysis combined high performance liquid chromatography (HPLC) technique and self-comparison method. Results: Based on the behavioral performance, the exhausting exercise process could be di-vided into 5 different stages, the rest condition, auto exercise period, early fatigue period, exhaustion condition and recovery period. The elec-trophysiological study results showed that, the coherence between neural activity in rat SNr, VL and SMA was significant between 0~30 Hz during all the procedure of exhausting exercise. Compared with the rest condition, the microdialysis study showed that the Glu concentrations and Glu/GABA ratio in SNr were decreased significantly during automatic exercise period (P < 0.05, P < 0.01), the GABA concentrations were increased significantly (P < 0.05, P < 0.01), while, in VL and cortex, the Glu concentrations and Glu/GABA ratio were increased significantly (P < 0.05, P < 0.01), the GABA concentrations were decreased significantly (P < 0.05, P < 0.01). Under early fatigue and ex-haustion conditions, compared with the rest condition,the Glu concentrations and Glu/GABA ratio in SNr were increased significantly (P < 0.05, P < 0.01), the GABA concentrations were decreased significantly (P < 0.05, P < 0.01), while the Glu concentrations and Glu/GABA ratio in VL and cortex were decreased significantly (P < 0.05, P < 0.01), the GABA concentrations were increased significantly (P < 0.05, P < 0.01). Conclusion: The neural net work communication between ‘substantianigra-ventralislateralis-cortex’ pathway exists, changes of Glu and GABA in the nucelus of the pathway are one of the factors resulting in the changes of neural activity.

Key words: exhausting exercise, rat, ‘substantianigra-ventralislateralis-cortex&rsquo, pathway, coherence, neurotransmitter

胡琰茹, 刘晓莉, 乔德才. 一次性力竭运动过程中大鼠“黑质-丘脑-皮层”通路神经元相干性及递质动态变化[J]. 中国应用生理学杂志, 2017, 33(3): 204-211.

HU Yan-ru, LIU Xiao-li, QIAO De-cai. Dynamic changes of ‘substantianigra-ventralislateralis-cortex’ pathway neural activity coherence and neurotransmitters in rat during exhausting exercise[J]. CJAP, 2017, 33(3): 204-211.

参考文献

[1] Middleton FA, Strick PL. Basal ganglia and cerebellar loops:motor and cognitive circuits[J]. Brain Res Brain Res Rev, 2000, 31(2-3):236-250.
[2] Nybo L, Secherb NH. Cerebral perturbations provoked by prolonged exercise[J]. Prog Neurobiol, 2004, 72(4):223-261.
[3] 胡琰茹, 刘晓莉, 乔德才. 大鼠在力竭运动过程中"黑质-丘脑-皮层"通路的调控作用研究[J]. 上海体育学院学报, 2013, 37(5):57-62.
[4] Meeusen R, Watson P, Dvorak J. The brain and fatigue:new opportunities for nutritional interventions[J]. J Sports Sci, 2006, 24(7):773-782.
[5] 王大磊, 刘晓莉, 乔德才. 大鼠力竭运动中丘脑底核和皮层神经元电活动的变化[J]. 中国应用生理学杂志, 2011, 27(4):427-431.
[6] 杨东升, 刘晓莉, 乔德才. 力竭运动及恢复期大鼠纹状体5-HT、DA及其代谢物浓度的动态变化研究[J]. 中国应用生理学杂志, 2011, 27(4):432-436.
[7] Mora F, Segovia G, Del Arco A. Glutamate-dopamine-GABA interactions in the aging basal ganglia[J]. Brain Res Rev, 2008, 58(2):340-353.
[8] Paxinos G, Watson C. The rat brain in stereotaxic coordinates[M]. 3rd Edition, San Diego:Academic, 1997:26-30,36-37,39-43.
[9] Taib NOB, Manto M. Effects of trains of high-frequency stimulation of the premotor/supplementary motor area on con-ditioned corticomotor responses in hemicerebellectomized rats[J]. Exp Neurol, 2008, 212(1):157-165.
[10] 刘晓莉, 罗勇, 乔德才. 大鼠一次性力竭跑台运动模型的建立及动态评价[J]. 中国实验动物学报, 2012, 20(3):25-28.
[11] 胡琰茹, 刘晓莉, 乔德才. 力竭运动大鼠苍白球内侧部神经递质浓度及相应受体表达变化研究[J]. 北京体育大学学报, 2015, 38(1):57-62,79.
[12] Wang D, Liu X, Qiao D. Modulatory effect of subthalamic nucleus on the development of fatigue during exhausting exer-cise:an in vivo electrophysiological and microdialysis study in rats[J]. J Sports Sci Med, 2012, 11(2):286-293.
[13] Hu Y, Liu X, Qiao D. Increased extracellular dopamine and 5-hydroxytryptamine levels contribute to enhanced subthalamic nucleus neural activity during exhausting exercise[J]. Biol Sport, 2015, 32(3):187-192.
[14] Colgin LL, Denninger T, Fyhn M, et al. Frequency of gam-ma oscillation routes flow of information in the hippocampus[J]. Nature, 2009, 462(7271):353-357.
[15] Del percio C, Iacoboni M, Lizio R, et al. Functional coupling of parietal alpha rhythms is enhanced in athletes before visuomotor performance:a coherence electroencephalographic study[J]. Neuroscience, 2011, 175(4):198-211.
[16] Fell J, Axmacher N. The role of phase synchronization in memory processes[J]. Nat Rev Neurosci, 2011, 12(2):105-118.
[17] Blomstrand E. A role for branched-chain amino acids in re-ducing central fatigue[J]. J Nutr, 2006, 136(2):544-547.
[18] Sophie S, Marco L, Francesco M, et al. In vivo neurochemi-cal effects of the NR2B selective NMDA receptor antagonist CR 3394in 6-hydmxydopamine lesioned rats[J]. Eur J Pharmacol, 2008, 584(2/3):297-305.
[19] Galeffi F, Bianchi L, Bolam JP, et al. The effect of 6-hy-droxydopamine lesions on the release of amino acids in the di-rect and indirect pathways of the basal ganglia:a dual micro-dialysis probe analysis[J]. Eur J Neurosci, 2003, 18:856-868.
[20] José A, Obeso, María C. Rodríguez-Oroz, Manuel Rodríguez, et al. The Basal ganglia and disorders of movement:patho-physiological mechanisms[J]. News PhysiolSci, 2002, 17(2):51-55.
[21] Dostrovsky JO, Levy R, Wu JP, et al. Microstimulation-in-duced inhibition of neuronal firing in human globuspallidus[J]. J Neurophysiol, 2000, 84(1):570-604.

一次性力竭运动过程中大鼠“黑质-丘脑-皮层”通路神经元相干性及递质动态变化

Dynamic changes of ‘substantianigra-ventralislateralis-cortex’ pathway neural activity coherence and neurotransmitters in rat during exhausting exercise

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