不同剂量玛咖对力竭运动致大鼠运动性低血糖的保护作用

doi:10.12047/j.cjap.5605.2018.054

中国应用生理学杂志 ›› 2018, Vol. 34 ›› Issue (3): 229-233.doi: 10.12047/j.cjap.5605.2018.054

不同剂量玛咖对力竭运动致大鼠运动性低血糖的保护作用

王彦英^1,2, 曹建民², 周海涛³, 张静³, 牛衍龙², 王灿², 李林蔚², 李思琪³

1. 北京物资学院, 北京 101149;
2. 北京体育大学, 北京 100084;
3. 北京联合大学生物化学工程学院, 北京 100023

收稿日期:2017-05-26 修回日期:2018-01-02 出版日期:2018-05-28 发布日期:2018-09-08
通讯作者: 周海涛,Tel:86-013611383040;E-maill:zsettle@sina.com E-mail:zsettle@sina.com
基金资助:
北京市朝阳区协同创新项目（CYXC1508）；北京联合大学"启明星"大学生科技创新项目（201611417022）

Protective effect of different dosage of Lepidium meyennii on exhaustive exercise-induced hypoglycemia in rats

WANG Yan-ying^1,2, CAO Jian-min², ZHOU Hai-tao³, ZHANG Jing³, NIU Yan-long², WANG Can², LI Lin-wei², LI Si-qi³

1. Beijing Wuzi University, Beijing 101149;
2. Beijing Sport University, Beijing 100084;
3. Biochemical Engineering College of Beijing Union University, Beijing 100023, China

Received:2017-05-26 Revised:2018-01-02 Online:2018-05-28 Published:2018-09-08
Supported by:
北京市朝阳区协同创新项目（CYXC1508）；北京联合大学"启明星"大学生科技创新项目（201611417022）

摘要/Abstract

摘要： 目的：探讨不同剂量玛咖对力竭运动致大鼠运动性低血糖的保护作用。方法：采用递增负荷力竭游泳训练的方法建立运动性低血糖动物模型。55只42 d龄雄性Wistar大鼠随机分为5组：①静止对照组（C组），②运动对照组（M组），③~⑤运动+低、中、高剂量玛咖组（LM I、LM Ⅱ，LM Ⅲ组），每组10只（剔除不符合实验要求的大鼠5只）。每天灌胃（ig）1次，LM各组灌胃玛咖的剂量为0.2，0.4，1.2 g/kg体重，灌胃体积为5 ml/kg体重，C、M组灌胃等体积生理盐水。运动大鼠采用递增负荷力竭游泳训练42 d，42 d力竭游泳训练后，测定体重、力竭游泳时间，取血、肝脏及深层股四头肌检测相关生化指标。结果：与C组比较，M组体重、血糖水平、肌糖原与肝糖原含量、肝细胞磷酸烯醇式丙酮酸羧激酶（phosphoenol pyruvate carboxy kinase，PEPCK）表达、肝细胞阳性染色累计吸光度均值等均明显降低（P<0.05或P<0.01）；力竭游泳时间无明显差异；血乳酸含量明显升高（P<0.01）。与M组比较，LM各组体重、血糖水平、肌糖原与肝糖原含量、肝细胞PEPCK表达、肝细胞阳性染色累计吸光度均值等均明显升高（P<0.05或P<0.01），力竭游泳时间明显延长（P<0.01），血乳酸含量明显降低（P<0.01）。与LM I组比较，LMⅢ组体重无明显差异，LM Ⅲ组血糖水平、肌糖原与肝糖原含量、肝细胞PEPCK表达、肝细胞阳性染色累计吸光度均值等均明显升高（P<0.05），力竭游泳时间明显延长，血乳酸含量明显降低（P<0.05）。结论：高剂量玛咖可有效抑制和延缓长时间、大负荷运动导致的运动性低血糖和运动性疲劳的发生与发展，其机制可能与优化肌糖原和肝糖原的储备量及上调糖异生限速酶PEPCK的表达，提高PEPCK活性，促进糖异生有关。

关键词: 玛咖, 力竭运动, 运动性低血糖, 限速酶, 磷酸烯醇式丙酮酸羧激酶, 大鼠

Abstract: Objective: To investigate the protective effects of different dosage of L. meyennii on hypoglycemia of rats induced by exhaustive exercise.Methods: The exercise-induced hypoglycemia animal model was established using the increasing load to exhaustion swimming training. Fifty-five male Wistar rats aged six-week were randomly divided into five groups:①control group (C group), ②exercise control group (M group), ③~⑤exercise+ low dose or medium dose or high dose L. meyennii group (LM I, LM Ⅱ, LM Ⅲ group), 10 rats in each group (5 rats were excluded). L. meyennii was given to LM groups once a day, the dosage was 0.2,0.4,1.2 g/kg body weight respectively, the volume was 5 ml/kg body weight, C and M group were given the same volume of normal saline intragastrically. The exercise rats were drilled in an exhaustive swimming training for 42 d with an increasing load. After 42 d exhaustive training, the body weight, exhaustive swimming time were determined, and related biochemical indicators were detected from samples of plasma, liver and deep strands of quadriceps.Results: The body weight, the level of blood glucose, muscle glycogen, hepatic glycogen, expression of PEPCK in hepatocytes and hepatocyte positive staining accumulated absorbance of M group were lower than those of C group (P<0.05 or P<0.01), there was no obvious difference between C and M groups in the time of exhaustive swimming. Compared with the C group, the content of blood lactate of M group was increased significantly (P<0.01). Compared with M group, body weight, the level of blood glucose, muscle glycogen, hepatic glycogen, expression of PEPCK in hepatocytes and hepatocyte positive staining accumulated absorbance of LM groups were obviously increased (P<0.05 or P<0.01), the time of exhaustive swimming was prolonged (P<0.01), and the content of blood lactate was decreased significantly(P<0.01). There was no difference between LM Ⅰ and LM Ⅲ group in body weight. Compared with LM I group, the level of blood glucose, muscle glycogen, hepatic glycogen, expression of PEPCK in hepatocytes and hepatocyte positive staining accumulated absorbance of LM Ⅲ group was obviously increased (P<0.05), the time of exhaustive swimming of LM Ⅲ group was prolonged, and the content of blood lactate of LM Ⅲ group decreased significantly (P<0.05).Conclusion: High dosage of L. meyennii may effectively suppress and delay the occurrence and development of exercise-induced hypoglycemia and fatigue induced by the long-time and intensive training, and the mechanism may be related to optimize of muscle glycogen and hepatic glycogen reserve in one hand. It is also related to up-regulates the limited enzyme of PEPCK expression and activity in the other hand. Finally it promotes the impact of gluconeogenesis.

Key words: Lepidium meyennii, exhaustive exercise, exercise-induced hypoglycemia, rate-limiting enzyme, phosphoenolpyruvate carboxykinase, rat

王彦英, 曹建民, 周海涛, 张静, 牛衍龙, 王灿, 李林蔚, 李思琪. 不同剂量玛咖对力竭运动致大鼠运动性低血糖的保护作用[J]. 中国应用生理学杂志, 2018, 34(3): 229-233.

WANG Yan-ying, CAO Jian-min, ZHOU Hai-tao, ZHANG Jing, NIU Yan-long, WANG Can, LI Lin-wei, LI Si-qi. Protective effect of different dosage of Lepidium meyennii on exhaustive exercise-induced hypoglycemia in rats[J]. CJAP, 2018, 34(3): 229-233.

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不同剂量玛咖对力竭运动致大鼠运动性低血糖的保护作用

Protective effect of different dosage of Lepidium meyennii on exhaustive exercise-induced hypoglycemia in rats

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