miRNA-191对前列腺癌的增殖、迁移侵袭能力的影响及其机制*

doi:10.12047/j.cjap.6031.2020.133

中国应用生理学杂志 ›› 2020, Vol. 36 ›› Issue (6): 637-641.doi: 10.12047/j.cjap.6031.2020.133

miRNA-191对前列腺癌的增殖、迁移侵袭能力的影响及其机制^*

聂永莉^1△, 汤世敏¹, 彭芳², 许涛³, 毛永荣⁴

1.国药汉江医院肿瘤科, 湖北丹江口 442700;
2.长江大学机能学部, 湖北荆州 434023;
3.十堰市人民医院肿瘤科, 湖北十堰 442000;
4.湖北省肿瘤医院病理科, 湖北武汉 430079

收稿日期:2020-03-06 修回日期:2020-11-09 出版日期:2020-11-28 发布日期:2021-03-15
通讯作者: ^△Tel: 13986873582; E-mail: nieyongli88@163.com
基金资助:
^*湖北省十堰市科学技术局科学技术研究与开发项目(16Y46)

miRNA-191 promotes proliferation, migration and invasion of prostate cancer by targeting PLCD1

NIE Yong-li^1△, TANG Shi-min¹, PENG Fang², XU Tao³, MAO Yong-rong⁴

1. Department of Oncology, Sinopharm Hanjiang Hospital, Danjiangkou 442700;
2. Department of Functionology, Yangtze University, Jingzhou 434023;
3. Department of Oncology, Renmin Hospital, Hubei University of Medicine, Shiyan 442000;
4. Department of Pathology, Hubei Cancer Hospital, Wuhan 430079, China

Received:2020-03-06 Revised:2020-11-09 Online:2020-11-28 Published:2021-03-15

摘要/Abstract

摘要： 目的:观察miRNA-191对前列腺癌的增殖、迁移和侵袭能力的影响,并探讨其机制。方法:分别检测4种人前列癌细胞系(PC-3、DU-145、LNCa P、22RU1)及人正常前列腺细胞RWPE-2中miRNA-191的表达水平,并选择前列腺癌细胞系PC-3作为实验对象。将PC-3细胞分为3组:空白对照组(不转染)、miRNA-191 NC组(Inhibitor NC转染PC-3细胞)、miRNA-191 Inhibitor组(miRNA-191 Inhibitor转染PC-3细胞),每组设置3个复孔。采用RT-qPCR法检测PC-3细胞miRNA-191和PLCD1的mRNA表达水平;采用CCK8法检测PC-3细胞增殖水平;采用划痕实验和侵袭实验分别检测PC-3细胞迁移能力和侵袭能力;通过Targetscan靶基因预测网站,筛选PLCD1作为miRNA-191的靶向蛋白,并用双荧光素酶靶标实验验证;采用Western blot法检测PC-3细胞PLCD1的蛋白表达。结果:与RWPE-2细胞相比,人前列癌细胞中miNRA-191的表达水平显著升高(P＜0.05),且miRNA-191的表达水平在PC-3中较其他3种细胞系显著上调(P＜0.05)。抑制miRNA-191的表达水平后,PLCD1表达水平显著升高,PC-3细胞增殖能力受到抑制,迁移和侵袭能力较空白对照组和miRNA-191 NC组显著降低(P＜0.05)。双荧光素酶报告基因实验结果显示,PLCD1基因是miRNA-191的靶基因。结论:miRNA-191通过靶向PLCD1促进前列腺癌PC-3细胞的增殖、迁移和侵袭能力。

关键词: 前列腺癌, miRNA-191, PLCD1, 增殖, 迁移, 侵袭

Abstract: Objective: To investigate the effects of miRNA-191 on the proliferation, migration and invasion of prostate cancer, and to explore its mechanism. Methods: The expression levels of miRNA-191 in four human prostate cancer cell lines (PC-3, DU-145, LNCa P, 22RU1) and human normal prostate cell line RWPE-2 were detected, and prostate cancer cell line PC-3 was selected as the experimental object. PC-3 cells were divided into three groups: blank control group (no transfection), miRNA-191 NC group (PC-3 cells transfected with Inhibitor NC) and miRNA-191 Inhibitor group (PC-3 cells transfected with miRNA-191 Inhibitor), and each group was provided with three multiple pores. The expression levels of miRNA-191 and PLCD1 were detected by RT-PCR. The cell proliferation was detected by CCK8 assay. Scratch test and invasive test were used to detect cell migration and invasive ability. Through Targetscan target gene prediction website, PLCD1 was screened as the target protein of miRNA-191, and verified by double luciferase target experiment.Western blot assay was used to detect the expression of PLCD1 in cells of each group. Results: Compared with RWPE-2 cells, the expression level of miRNA-191 in human prostate cancer cells was significantly higher (P ＜0.05), and the expression level of miRNA191 in PC-3 was significantly higher than that in other three cell lines (P＜0.05). After inhibiting the expression of miRNA-191, the expression levels of PLCD1 was significantly higher while PC-3 cells' proliferation ability was inhibited, and their migration and invasion ability were significantly lower than those of blank control group and miRNA-191 NC group (P＜ 0.05). The results of double luciferase reporter gene assay showed that PLCD1 gene was a target gene of miRNA-191. Conclusion: miRNA-191 promote the proliferation, migration and invasion of prostate cancer PC-3 cells by targeting PLCD1.

Key words: prostate cancer, miRNA-191, PLCD1, proliferation, migration, invasion

中图分类号:

R737.25

聂永莉, 汤世敏, 彭芳, 许涛, 毛永荣. miRNA-191对前列腺癌的增殖、迁移侵袭能力的影响及其机制^*[J]. 中国应用生理学杂志, 2020, 36(6): 637-641.

NIE Yong-li, TANG Shi-min, PENG Fang, XU Tao, MAO Yong-rong. miRNA-191 promotes proliferation, migration and invasion of prostate cancer by targeting PLCD1[J]. CJAP, 2020, 36(6): 637-641.

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miRNA-191对前列腺癌的增殖、迁移侵袭能力的影响及其机制^*

miRNA-191 promotes proliferation, migration and invasion of prostate cancer by targeting PLCD1

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