不同浓度桦木酸对人胃癌MGC-803细胞自噬的影响

doi:10.12047/j.cjap.6054.2021.001

中国应用生理学杂志 ›› 2021, Vol. 37 ›› Issue (3): 254-256.doi: 10.12047/j.cjap.6054.2021.001

不同浓度桦木酸对人胃癌MGC-803细胞自噬的影响

王琪¹, 邵淑丽^1,2Δ, 何孟奇¹, 黄鑫^1,2, 张伟伟^1,2, 张珍珠^1,2

1. 齐齐哈尔大学生命科学与农林学院, 黑龙江齐齐哈尔 161006;
2. 抗性基因工程与寒地生物多样性保护黑龙江省重点实验室, 黑龙江齐齐哈尔 161006

出版日期:2021-05-28 发布日期:2021-08-09
通讯作者: ^△Tel: 13836267458; E-mail: shshl32@163.com
基金资助:
^*齐齐哈尔大学黑龙江省教育厅基本业务专项重点项目(135109104);黑龙江省高教强省优势特色学科—玉米“粮头食尾”重点专项(LTSW201737);黑龙江省省属高等学校基本科研业务费科研项目(植物性食品加工技术特色学科专项)(YSTSXK201809)

Effects of betulinic acid at different concentrations on autophagy of human gastric cancer MGC-803 cells

WANG Qi¹, SHAO Shu-li ^1,2Δ, HE Meng-qi¹, HUANG Xin ^1,2, ZHANG Wei-wei ^1,2, ZHANG Zhen-zhu^1,2

1. College of Life Science and Agriculture and Forestry, Qiqihar University, Qiqihaer 161000;
2. Heilongjiang Provincial Key Laboratory of Resistance Gene Engineering and Protection of Biodiversity in Cold Areas, Qiqihar University, Qiqihaer 161006, China

Online:2021-05-28 Published:2021-08-09

摘要/Abstract

摘要： 目的: 本研究利用不同浓度桦木酸处理人胃癌MGC-803细胞,以探究其对细胞自噬的影响。方法: 将人胃癌MGC-803细胞分为4组,每组3个复孔,对照组不加桦木酸处理,其余三组分别加入终浓度为10、20、30 mg/L桦木酸。桦木酸处理细胞48 h后,qRT-PCR检测桦木酸对人胃癌MGC-803细胞自噬相关基因mRNA表达的影响。Western blot检测药物处理细胞自噬相关基因的蛋白表达。利用免疫荧光检测药物处理后MGC-803细胞内LC3蛋白的胞内定位及表达。结果: 与对照组相比,在10～30 mg/L浓度范围内,桦木酸处理的人胃癌MGC-803细胞LC3、Beclin-1 mRNA的表达明显升高(P＜0.05),Beclin-1、LC3-Ⅱ蛋白的表达显著升高(P＜0.05),LC3-Ⅰ蛋白的表达明显降低(P＜0.05),其中30 mg/L处理组表现最佳。此外,桦木酸还可诱导MGC-803细胞LC3蛋白在细胞质内形成点状聚集。结论: 在10～30 mg/L浓度范围内,桦木酸能诱导人胃癌MGC-803细胞发生自噬。

关键词: 桦木酸, 人胃癌MGC-803, 自噬

Abstract: Objective: In this study, human gastric cancer MGC-803 cells were treated with betulinic acid at different concentrations to investigate its effect on cell autophagy. Methods: The human gastric cancer MGC-803 cells were divided into 4 groups, each group was set with 3 replicate. The control group was not treated with betulinic acid, the other three groups were added with final concentration of 10,20,30 mg/L betulinic acid, respectively. Cells were treated with betulinic acid for 48 h,qRT-PCR was applied to detect the effect of betulinic acid on mRNA expressions of autophagy-related genes in human gastric cancer MGC-803 cells. Western blot was performed to determine the protein expressions of cell autophagy-related genes after drug treatment. Immunofluorescence was used to detect the localization and expression of LC3 protein in MGC-803 cells after drug treatment. Results: Compared with the control group,in the concentration range of 10~30 mg/L, the mRNA expression of LC3 and Beclin-1 in human gastric cancer MGC-803 cells treated with betulinic acid were increased significantly, the expressions of Beclin-1 and LC3-Ⅱ protein were also increased significantly, while the expression of LC3-Ⅰ protein was decreased significantly. Among them, betulinic acid at the concentration of 30 mg/L showed the best effects. In addition, betulinic acid induced the LC3 protein in MGC-803 cells to form spot aggregates in the cytoplasm. Conclusion: At the concentrations of 10~30 mg/L, betulinic acid can induce autophagy in human gastric cancer MGC-803 cells.

Key words: betulinic acid, human gastric cancer MGC-803, autophagy

中图分类号:

Q291

王琪, 邵淑丽, 何孟奇, 黄鑫, 张伟伟, 张珍珠. 不同浓度桦木酸对人胃癌MGC-803细胞自噬的影响[J]. 中国应用生理学杂志, 2021, 37(3): 254-256.

WANG Qi, SHAO Shu-li, HE Meng-qi, HUANG Xin, ZHANG Wei-wei, ZHANG Zhen-zhu. Effects of betulinic acid at different concentrations on autophagy of human gastric cancer MGC-803 cells[J]. CJAP, 2021, 37(3): 254-256.

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不同浓度桦木酸对人胃癌MGC-803细胞自噬的影响

Effects of betulinic acid at different concentrations on autophagy of human gastric cancer MGC-803 cells

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