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CJAP ›› 2018, Vol. 34 ›› Issue (5): 385-388.doi: 10.12047/j.cjap.5668.2018.088

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Effects of TGF-β1 on the expression and secretion of cytokines induced by Aβ1-42 in hippocampal neuron and microglia co-cultures

FANG Xiao-xia1,2, ZHOU Yi1, SUN Gao-lin1, QIU Yi-hua1, PENG Yu-ping1   

  1. 1. Department of Physiology, School of Medicine, Nantong University, Nantong 226001, China;
    2. Function Laboratory, School of Medicine, Nantong University, Nantong 226001, China
  • Received:2018-01-05 Revised:2018-08-02 Online:2018-09-28 Published:2019-02-21
  • Supported by:
    国家自然科学基金项目(31371182);江苏省高等学校自然科学研究面上项目(18KJD310003);江苏省教育厅研究生科研与实践创新计划项目(KYCX18_2399)

Abstract: Objective: To investigate the neuroprotective effects of transforming growth factor beta 1(TGF-β1) on the expression and secretion of cytokines induced by Aβ1-42 in hippocampal neurons and microglial co-cultures. Methods: Hippocampal neurons and microglia obtained from SD rat were co-cultured. TGF-β1 was applied on day 5 after the neurons and microglia co-cultures were incubated at the concentrations of 5 or 20 ng/ml, Aβ1-42 was added 1 h following TGF-β1 application at a concentration of 5 μmol/L. They were incubated for 72 h and then assessed for further studies. Western blot analyses were employed to examine the expression of inducible nitric oxide synthase (iNOS); Real-time PCR and ELISA were used to detect the mRNA expression and secretion of tumor necrosis factor-α (TNF-α), interleukin-1β (IL-1β) and insulin-like growth factor-1 (IGF-1). Results: In the hippocampal neuron-microglia co-cultures, Aβ1-42 induced upregulation of iNOS, TNF-α and IL-1β, downregulation of IGF-1. TGF-β1 pretreatment ameliorated the pro-inflammatory effects caused by Aβ1-42. Conclusion: TGF-β1 significantly inhibits the increase in inflammatory cytokines and the decrease in neurotrophic factor which are caused by Aβ1-42-induced microglia activation.

Key words: TGF-β1, 1-42, microglia, hippocampal neurons, cytokines

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