Effects of Genipin on the expression of uncoupling protein 1 in brown  adipose and white adipose tissues in mice

doi:10.12047/j.cjap.5692.2019.018

Abstract

Abstract: Objective:To investigate the effects of genipin on promoting brown adipose tissue activation and white adipose tissue browning.Methods: The male C57BL/6J mice were divided into three groups: normal control group, genipin group and cold-stimulus group.Genipin group were treated consecutively with genipin at a dose of 15 mg/kg once a day for 9 days, normal control group were treated with the saline.The mice with cold-stimulus were exposed to 4℃ environment for 5 days.Daily food amount and body weight were measured.Morphological changes were observed in the subscapular region, inguinal region and epididymis around the adipose tissue.The expression of uncoupling protein 1 (UCP1) was determined by real-time PCR and Western blot respectively.Results: The wet weight of white fat in genipin-treated mice was decreased by 16% , and 28% in that of cold-stimulus mice, compared with the normal control group (P＜0.05).After treatments of genipin and cold-stimulus, the color of white adipose tissues was darker, and the size of lipid droplets in adipocytes was smaller, whereas the number was increased.Compared with the normal control group, UCP1 expression was increased obviously in fat tissues, including the subcutaneous and visceral white adipose tissues, and brown adipose tissue after treated with genipin and cold-stimulus (P＜0.05).Conclusion: Genipin promoted activation of brown adipose tissue and browning of white adipose tissue by upregulating UCP1 expression, which could contribute to the loss of body weight against obesity.

Key words: genipin, cold-stimulus, brown adipose tissue, white adipose tissue, uncoupling protein 1, mice

SHEN Na-na, GONG De-zheng, ZOU Feng-kai, YAN Yu, GUAN Li-li, ZOU Yuan. Effects of Genipin on the expression of uncoupling protein 1 in brown adipose and white adipose tissues in mice[J]. CJAP, 2019, 35(1): 74-78.

References

[1] Veses AM, Martínez-Gómez D, Gómez-Martínez S, et al. Physical fitness, overweight and the risk of eating disorders in adolescents [J]. Pediatr Obes, 2014, 9(1): 1-9.
[2] Cinti S. The adipose organ at a glance [J]. Dis Model Mech, 2012, 5(5): 588-594.
[3] 许冬明, 李显国. 高脂饮食及运动对2型糖尿病大鼠脂联素、瘦素及血糖水平的影响[J]. 中国应用生理学杂志, 2016, 32(4): 298-300.
[4] Calvani R, Leeuwenburgh C, Marzetti E.Brown adipose tissue and the cold war against obesity [J]. Diabetes, 2014, 63(12): 3998-4000.
[5] Kim SJ, Kim JK, Lee DU, et al. Genipin protects lipopolysaccharide-induced apoptotic liver damage in D-galactosamine-sensitized mice [J]. Eur J Pharmacol, 2010, 635(1-3): 188-193.
[6] Rajanbabu V, Galam L, Fukumoto J, et al. Genipin suppresses NLRP3 inflammasome activation through uncoupling protein-2 [J]. Cell Immunol, 2015, 297 (1): 40-45.
[7] Guan L, Feng H, Gong D, et al. Genipin ameliorates age-related insulin resistance through inhibiting hepatic oxidative stress and mitochondrial dysfunction [J]. Exp Gerontol, 2013, 48(12): 1387-1394.
[8] Mahgoub E, Kumaraswamy SM, Kader KH, et al. Genipin attenuates cisplatin-induced nephrotoxicity by counteracting oxidative stress, inflammation, and apoptosis [J]. Biomed Pharmacother, 2017, 93: 1083-1097.
[9] Dando I, Pacchiana R, Pozza ED, et al. UCP2 inhibition induces ROS/Akt/mTOR axis: Role of GAPDH nuclear translocation in genipin/everolimus anticancer synergism [J]. Free Radic Biol Med, 2017, 113: 176-189.
[10]吕艺, 杜桂仙. 对冷适应大鼠棕色脂肪和肝脏在非寒颤产热机制中作用的评价[J]. 中国应用生理学杂志, 1992, 8(3): 237-241.
[11]Langin, D Recruitment of brown fat and conversion of white into brown adipocytes: strategies to fight the metabolic complications of obesity[J]? Biochim Biophys Acta, 2010, 1801(3): 372-376.
[12]Sharp LZ, Shinoda K, Ohno H, et al. Human BAT possesses molecular signatures that resemble beige/brite cells[J]. Plos One, 2012, 7(11): e49452.
[13]Rosenwald M, Perdikari A, Rülicke T, et al. Bi-directional interconversion of brite and white adipocytes [J]. Nat Cell Biol, 2013, 15(6): 659-667.
[14]Bartelt A, Heeren J. Adipose tissue browning and metabolic health [J]. Nat Rev Endocrinol, 2014, 10(1): 24-36.
[15]Jia R, Luo XQ, Wang G, et al. Characterization of cold-induced remodelling reveals depot-specific differences across and within brown and white adipose tissues in mice [J]. Acta Physiol, 2016, 217(4): 311-324.
[16]Zhang Z, Zhang H, Li B, et al. Berberine activates thermogenesis in white and brown adipose tissue [J]. Nat Commun, 2014, 5: 5493.
[17]Qian SW, Tang Y, Li X, et al. BMP4-mediated brown fat-like changes in white adipose tissue alter glucose and energy homeostasis [J]. Proc Natl Acad Sci USA, 2013, 110(9): E798-807.
[18]Lim S, Honek J, Xue Y, et al. Cold-induced activation of brown adipose tissue and adipose angiogenesis in mice [J]. Nat Protoc, 2012, 7(3): 606-615.
[19]Elias I, Franckhauser S, Ferré T, et al. Adipose tissue overexpression of vascular endothelial growth factor protects against diet-induced obesity and insulin resistance [J]. Diabetes, 2012, 61(7): 1801-1813.
[20]Sun K, Wernstedt Asterholm I, Kusminski CM, et al. Dichotomous effects of VEGF-A on adipose tissue dysfunction [J]. Proc Natl Acad Sci USA, 2012, 109(15): 5874-5879.
[21]Seale P, Kajimura S, Yang W, et al. Transcriptional control of brown fat determination by PRDM16 [J]. Cell Metab, 2007, 6(1): 38-54.

Effects of Genipin on the expression of uncoupling protein 1 in brown adipose and white adipose tissues in mice

PDF (PC)

Knowledge

Abstract

Cite this article

share this article

References

Related Articles 15

Recommended Articles

Metrics

Comments

[1]	SHAO Xiao-ting, HU Jing, ZHANG Xin-ying, ZHAO Bing-bing, LI Si-wei, GAO Ping, XU Chang-qing, WEI Can. Effect of exogenous spermine pretreatment on alleviating renal fibrosis in diabetic nephropathy mice and its related mechanism [J]. CJAP, 2020, 36(3): 207-210.
[2]	JIANG Hong-bo, DONG Jia-xing, QIN Yu-fei, LIU Jia-cong, JIANG Wan-ju, LI Ruo-nan, LIU Lan-ci, TIAN Yi-dan, XU Yu-ming, DU Ai-lin. Effects of NaHS on MBP and learning and memory in hippocampus of mice with spinocerebellar ataxia [J]. CJAP, 2020, 36(3): 235-239.
[3]	FANG Zhen, HU Xi-hou, LI Kang, HAN Jie, TIAN Lei, YAN Jun, ZHANG Wei, LAI Wen-qing, LIN Ben-cheng, LIU Xiao-hua, XI Zhu-ge. Inflammatory mechanism of hippocampal tissue injury induced by PM_2.5 in nasal drip in mice [J]. CJAP, 2020, 36(3): 240-244.
[4]	WANG Feng, ZHANG Hai-feng. Effects of liraglutide combined with vitamin D on non-alcoholic fatty liver induced by high fat in mice and its mechanism [J]. CJAP, 2020, 36(3): 261-264.
[5]	LUO Wei, AI Lei, WANG Bo-fa, WANG Li-ying, GAN Yan-ming, ZHOU Yue. Effects of mice macrophages on skeletal muscle cells under high glucose treatment [J]. CJAP, 2020, 36(2): 124-129.
[6]	ZHAO Yang, YANG Sheng-chang, YU Fu-yang, LI Wen-ya, JI En-sheng. Effects of Xiaotan Huayu Liqiao Formula on cognitive impairment in mice exposed to chronic intermittent hypoxia [J]. CJAP, 2020, 36(2): 143-147.
[7]	LUO Yi-shuang, ZHENG Xiu-ting, ZHANG Hao-yue, XU Li-ping, QIU Jia-peng, XU Gang-ming, LIU Ai-ming. The cholestatic fibrosis induced by α-naphthylisothiocyanate in mice and the inflammation pathway [J]. CJAP, 2020, 36(2): 152-156.
[8]	LUO Jin-ding, WU Di, LYU Hui-jie, HE Jian-qin, YANG Si-si, FENG Shui-dong, LING Hong-yan. Effects of dihydromyricetin on high fat diet induced obesity in mice and its mechanism [J]. CJAP, 2020, 36(1): 6-11.
[9]	LU Man-man, CHEN Jia-yao, NIU Ming-yu, WU Wei-dong. Effect of aerobic exercise on the expression of CLK2 protein in liver of mice fed with high fat diet [J]. CJAP, 2020, 36(1): 23-26.
[10]	HUI Yi, YAN Shu-guang, WANG Qian, LI Jing-tao, WEI Hai-liang, SHAN Yu-peng. Effects of 6-Shogaol on Notch signaling pathway in colonic epithelial cells of ulcerative colitis mice [J]. CJAP, 2020, 36(1): 90-93.
[11]	HE Feng-qin, XIANG Quan-li, QU Geng-chao, ZHOU Deng-xiang. Effects of Soy isoflavones on depression like behavior in female mice and mechanism of estrogen receptor β [J]. CJAP, 2019, 35(6): 486-490.
[12]	MA Shao-shuai, CHEN Hui-yu, LIANG Zhi-dong. Intervention effect of cassia seed decoction combined with 4 weeks swimming training on exercise induced fatigue in mice [J]. CJAP, 2019, 35(6): 522-524.
[13]	Maimaiti 穀isireyili, Aikebaier稟ili, Maimaitiaili稟izezi, Wubulikasimu稺ulamu, LI Yi-liang, Aziguli�Alimujiang, YAN Jing, Kelimu稟budureyimu. Effects of psychological stress on xanthine oxidase expression, activity and related markers in adipose tissue of mice [J]. CJAP, 2019, 35(6): 537-542.
[14]	TANG Yi-hao, YIN Cheng-zhe, LI Kai, LI Wei-xin, FAN Chun-fang. Protective effects of azithromycin on adriamycin-induced nephropathy with albumin overload in mice [J]. CJAP, 2019, 35(6): 548-550.
[15]	JIANG Pan-ruo, LOU En-zhe, ZHAO Zhi-xiang, XU Jiong-nan, ZHENG Xiao-fei, KE Rui-jun, CHEN Jia-yu. Inhibitory effect of celery polysaccharide on lung cancer in mice [J]. CJAP, 2019, 35(6): 551-554.