The effects of aerobic exercise on ERK1/2 activity in skeletal muscle of type 2 diabetic rats

doi:10.12047/j.cjap.5448.2017.008

Abstract

Abstract: Objective: To observe the effects of aerobic exercise on activity of extracellular signal-regulated kinase (ERK1/2) in skeletal muscle of type 2 diabetic rats and explore the prevention and control mechanism of aerobic exercise on type 2 diabetes.Methods: Seventy five SD rats were randomly divided into:normal control group(CON) including 15 rats was fed with normal diet, diabetes control group 1(DC1), diabetes exercise group 1(DE1), diabetes control group 2(DC2), diabetes exercise group 2(DE2). Diabetes model group were fed with high-fatty and high-sugar diet. The diabetes model rats were fed with high-fatty and high-sugar diet for 8 weeks,.Diabetes group 2 rats were injected intraperitoneal streptozotocin(STZ)to induce type 2 diabetes. At the early stage of last swimming week, diabetes exercise group1 and diabetes control group 1 were injected with STZ (35 mg/kg) at the same time, After three days, if the level of blood glucose was ≥ 16.7mmol/L, the model was successful. After 8 week-interventions, all the rats were killed, the serum levels of insulin and the expression of ERK1/2 protein in skeletal muscle were determined.Results: ①Compared with the normal control group, the levels of total cholesterol(TC), triglyceride(TG), low density lipoprotein cholesterin(LDL-C), free fatty acid(FFA), fasting blood-glucose(FBG), fasting insulin(FIN) and insulin resistance index(HOMA-IR) were increased significantly in diabetes control group(P<0.05 or 0.01). However, the expression of ERK1/2 phosphorylation protein was decreased obviously in diabetes control group. The content of ERK1/2 protein was decreased obviously in diabetes control group 2 had (P<0.05). 2.After eight weeks' swimming, compared with the diabetes control group, the levels of TC,TG, FFA, LDL-C, FBG, FIN and HOMA-IR were decreased significantly in diabetes exercise group(P<0.05 or 0.01). At the same time, the expression of ERK1/2 phosphorylation protein was increased obviously in diabetes exercise group(P<0.05).Conclusion: Long-term aerobic exercise can improve the skeletal muscle ERK1/2 phosphorylation and insulin resistance of type 2 diabetic rats, thereby lowering blood glucose. It is probably one of the mechanisms to improve glucose metabolism disorders and insulin sensitivity.

Key words: aerobic exercise, insulin resistance, type 2 diabetes, ERK1/2

LI Yan-hui, YI Xue-jie. The effects of aerobic exercise on ERK1/2 activity in skeletal muscle of type 2 diabetic rats[J]. CJAP, 2017, 33(1): 33-37.

References

[1] 李锐. Ⅱ型糖尿病患者运动疗法的研究进展[J]. 哈尔滨体育学院学报, 2014, 32(6):92-96.
[2] 周静, 高晟, 江立新, 等. 中药及其有效成分治疗胰岛素抵抗的分子机制研究进展[J]. 天津药学, 2015, 27(3):57-61.
[3] Cartee GD. Mechanisms for greater insulin-stimulated glucose uptake in normal and insulin-resistant skeletal muscle after a-cute exercise[J]. Am J Physiol Endocrinol Metab, 2015, 309(12):E949-959.
[4] Cusi K, Maezono K, Osman A, et al. Insulin resistance dif-fe rentially affects the PI3-Kinase and MAP Kinase-mediated signaling in human muscle[J]. J Clin Invest, 2000, 105(3):311-320.
[5] 吴军发, 吴毅, 胡永善, 等. 运动改善原发2型糖尿病大鼠血糖和血脂代谢紊乱的实验研究[J]. 中华物理医学与康复杂志, 2002, 24(9):536-538.
[6] 马泽兵, 胡晨, 罗强, 等. 胰岛素抵抗及其运动干预的研究进展[J]. 辽宁体育科技, 2014, 36(3):44-47.
[7] Kyriakis JM, Avruch J. Mam malian mitogen-activated pro-tein kinases signal transduction pathways activated by stress and inflammation[J]. Physiol Rev, 2001, 81(2):807-869.
[8] Ren ZQ, Zhang PB, Zhang XZ, et al. Duodenal-jejunal ex-clusion improves insulin resistance in type 2 diabetic rats by upregulating the hepatic insulin signaling pathway[J]. Nutri-tion, 2015, 31(5):733-739.
[9] 马连环, 刘建. ERK1/2的研究进展[J]. 国外医学(生理、病理科学与临床分册), 2005, 25(3):279-282.
[10] Sakamoto K, Goodyear LJ. Invited review:Intracellular sig-naling in contracting skeletal muscle[J]. J Appl Physiol, 2002, 93(1):369-383.
[11] Gorin Y, Ricono JM, Wagner B, et al. Angiotensin -in-duced ERK1/2 activation and protein synthesis are redox-de-pendent in glomerular mesangial cells[J]. Biochem J, 2004, 381(Pt1):231-239.
[12] 吴毅. 运动疗法改善糖尿病大鼠糖代谢异常作用机制的实验研究[D]. 上海, 复旦大学:2007.
[13] Chen R, Li X, Lu S, et al. Role of extracelluar signal-regu-lated kinases 1 and 2 p38 mitogen-activated protein kinase pathways in regulating replication of Peniclilium marneffein in human macrophages[J]. Microbes Inf ect, 2014, 16(5):401-408.
[14] 王小娟, 李建华, 边仁秀. 有氧运动对2型糖尿病大鼠腓肠肌氧化应激及MAPKs信号通路的影响[J]. 中国运动医学杂志, 2012, 31(9):800-805.
[15] Goodyear LJ, Chang PY, Sherwood DJ, et al. Effects of ex-ercise and insulin on mitogen-activated protein kinase signal-ing pathways in rat skeletal muscle[J]. Am J Physiol, 1996, 271(2Pt1):E403-408.
[16] 曹师承. 运动对大鼠骨骼肌PI3K/AKt/mTor与MAPK 信号转导途径的影响[D]. 沈阳, 中国医科大学:2007.