Abstract: Objective: To study the effects of adiponin receptor agonist (AdipoRon) on renal injury in type 2 diabetic mice. Methods: The experiment was carried out on 40 SPF C57/BL6 male mice and they were randomly divided into normal control group (n=10) and experimental group (n=30). Mice in experimental group were given with high sugar and high fat feed in combination with only an intraperitoneal injection of small dose of streptozotocin to build the model of type 2 diabetes (T2DM), which were randomly divided into three groups, model control group (DM), low dose AdipoRon group (DM + L) and high dose AdipoRon group (DM+H)(n=10). Then the change of blood glucose was detected. The serum levels of insulin receptor (INSR), insulin receptor substrate-1 (IRS-1) and tumor necrosis factor-α (TNF-α) in mice were measured by ELASA. Pathological changes of renal tissues were observed with a light microscope after HE staining. The expressions of pancreatic duodenal homebox-1 (PDX-1) and insulin mRNA in renal tissues were detected by RT-PCR. The content of phosphated insulin receptor substrate-1 (p-IRS-1) protein in the kidney was determined by Western blot. Results: Compared with DM mice, blood glucose and TNF-α levels in DM + H mice and DM + L mice were significantly reduced (P<0.05), while the expressions of INSR,IRS-1 and the content of p-IRS-1 were increased markedly(P<0.05), and the expressions of PDX-1 and insulin mRNA in renal tissue were increased significantly(P<0.05, P<0.01). Conclusion: Mice treated with AdipoRon have lower blood glucose and TNF-α levels, and higher protein expression levels of INSR, IRS-1, and higher mRNA expression levels of PDX-1 and insulin, and the content of p-IRS-1. All of these indicate that AdipoRon has a certain effects on renal injury in type 2 diabetic mice.

Key words: AdipoRon, type 2 diabetes, kidney, insulin receptor, mice