The effects of prenatal stress on the astrocytes after cerebral ischemia/reperfusion injury in adult offspring rats

doi:10.13459/j.cnki.cjap.2016.04.004

CJAP ›› 2016, Vol. 32 ›› Issue (4): 301-304.doi: 10.13459/j.cnki.cjap.2016.04.004

Previous Articles Next Articles

The effects of prenatal stress on the astrocytes after cerebral ischemia/reperfusion injury in adult offspring rats

WANG Ling-xing, HUANG Hong-hong, CHEN Ya-fang, CAI Hong-chao, QIAN Jia-qiang

Department of Neurology, the Second Affiliated Hospital of Fujian Medical University, Quanzhou 362000, China

Received:2015-11-24 Revised:2016-04-04 Online:2016-07-28 Published:2018-06-20
Supported by:
福建省自然科学基金项目（2015J01449）；福建省教育厅项目（JB13067）；泉州市技术研究与开发项目（2012Z35）；院苗圃基金（2012MP65）

Abstract

Abstract: Objective:To investigate the effects of prenatal stress on astrocytes after ischemia/reperfusion of cerebral middle artery in adult offspring rats. Methods:Pregnant rats were randomly assigned to prenatal stress treatment group, which was exposed to restraint three times daily in the last week of pregnancy, and no prenatal stress treatment group. Adult male offspring rats were subjected to transient focal cerebral ischemia by middle cerebral artery occlusion (MCAO). There were three groups:prenatal stress+sham group, MCAO group and prenatal stress+MCAO group (n=10). After 5 days of reperfusion, the infarct size was evaluated. The morphology of astrocytes, co-local-ization of erythropoietin-producing hepatocellular receptor A4 (EphA4) and glial fibrillary acidic protein (GFAP) were detected by double im-munofluorescent staining. And the protein expressions of EphA4, GFAP and Neurocan in peri-ischemic regions were detected by Western blot. Results:The infarct size and the expression of EphA4, GFAP and Neurocan were significantly increased in prenatal stress+MCAO group compared with MCAO group (all P<0.05). And the morphological changes of GFAP-positive astrocytes and co-localization of EphA4/GFAP were more obvious in prenatal stress+MCAO group compared with MCAO group. Conclusion:Prenatal stress may upregulate the expression of EphA4 on astrocytes in the offspring rats after cerebral ischemia/reperfusion, which promotes the reactivity of astrocyte and increases the ex-pression of neurocan.

Key words: prenatal stress, offspring rats, cerebral ischemia, astrocyte

WANG Ling-xing, HUANG Hong-hong, CHEN Ya-fang, CAI Hong-chao, QIAN Jia-qiang. The effects of prenatal stress on the astrocytes after cerebral ischemia/reperfusion injury in adult offspring rats[J]. CJAP, 2016, 32(4): 301-304.

References

[1] Rolls A, Shechter R, Schwartz M. The bright side of the glial scar in CNS repair[J]. Nat Rev Neurosci, 2009, 10(3):235-241.
[2] Teshigawara K, Kuboyama T, Shigyo M, et al. A novel compound, denosomin, ameliorates spinal cord injury via ax-onal growth associated with astrocyte-secreted vimentin[J]. Br J Pharmacol, 2013, 168(4):903-919.
[3] Shen LH, Li Y, Gao Q, et al. Down-regulation of neurocan expression in reactive astrocytes promotes axonal regeneration and facilitates the neurorestorative effects of bone marrow stromal cells in the ischemic rat brain[J]. Glia, 2008, 56(16):1747-1754.
[4] 王凌星, 黄红红, 陈雅芳, 等. 产前应激对成年子代大鼠缺血性卒中后细胞凋亡的影响[J]. 中国应用生理学杂志, 2015, 31(5):427-430.
[5] Coulthard MG, Morgan M, Woodruff TM, et al. Eph/Ephrin signaling in injury and inflammation[J]. Am J Pathol, 2012, 181(5):1493-1503.
[6] Goldshmit Y, Spanevello MD, Tajouri S, et al. Epha4 blockers promote axonal regeneration and functional recovery fo llowing spinal cord injury in mice[J]. PLoS One, 2011, 6(9):e24636.
[7] Parmentier-Batteur S, Finger EN, Krishnan R, et al. Atten-uation of scratch-induced reactive astrogliosis by novel Epha4 kinase inhibitors[J]. J Neurochem, 2011, 118(6):1016-1031.
[8] Igosheva N, Taylor PD, Poston L, et al. Prenatal stress in the rat results in increased blood pressure responsiveness to stress and enhanced arterial reactivity to neuropeptide Y in adulthood[J]. J Physiol, 2007, 582(Pt 2):665-674.
[9] Longa EZ, Weinstein PR, Carlson S, et al. Reversible mid-dle cerebral artery occlusion without craniectomy in rats[J]. Stroke, 1989, 20(1):84-91.
[10] Swanson RA, Morton MT, Tsao-Wu G, et al. A semiauto-mated method for measuring brain infarct volume[J]. J Cereb Blood Flow Metab, 1990, 10(2):290-293.
[11] 尚宇, 吕红梅, 顾佩菲. 异丙酚对局灶性脑缺血/再灌注星形胶质细胞GFAP表达的影响[J]. 中国应用生理学杂志, 2009, 25(1):16-17.
[12] Frugier T, Conquest A, McLean C, et al. Expression and activation of Epha4 in the human brain after traumatic injury[J]. J Neuropathol Exp Neurol, 2012, 71(3):242-250.
[13] Goldshmit Y, Bourne J. Upregulation of Epha4 on astrocytes potentially mediates astrocytic gliosis after cortical lesion in the marmoset monkey[J]. J Neurotrauma, 2010, 27(7):1321-1332.
[14] Sofroniew MV. Molecular dissection of reactive astrogliosis and glial scar formation[J]. Trends Neurosci, 2009, 32(12):638-647.
[15] Asher RA, Morgenstern DA, Fidler PS, et al. Neurocan is upregulated in injured brain and in cytokine-treated astrocytes[J]. J Neurosci, 2000, 20(7):2427-2438.
[16] Deguchi K, Takaishi M, Hayashi T, et al. Expression of neurocan after transient middle cerebral artery occlusion in adult rat brain[J]. Brain Res, 2005, 1037(1-2):194-199.

The effects of prenatal stress on the astrocytes after cerebral ischemia/reperfusion injury in adult offspring rats

PDF (PC)

Knowledge

Abstract

Cite this article

share this article

References

Related Articles 4

Recommended Articles

Metrics

Comments

[1]	YANG Mei-li, HONG Shan-yan, HUANG Hong-hong, LYU Guo-rong, WANG Ling-xing. The effects of prenatal radiation of mobile phones on white matter in cerebellum of rat offspring [J]. CJAP, 2020, 36(1): 77-81.
[2]	DONG Yuan-yuan, WANG Lin, CHU Xu, CUI Shuai, KONG Qing-xia. Altered expressions of SphK1 and S1PR2 in hippocampus of epileptic rats [J]. CJAP, 2019, 35(4): 308-311.
[3]	WANG Ling-xing, HUANG Hong-hong, LV Guo-rong. cts of prenatal exposure of 850-1900MHz mobile phone on the expression of PCNA and DCX in dentate gyrus of offspring rats [J]. CJAP, 2018, 34(1): 35-38.
[4]	SI Yi-ling, ZHANG Jin-ying, DENG Zi-hui, XUE Hui, YAN Guang-tao. The effects of leptin on neuron apoptosis in mice with cerebral ischemia/reperfusion injury [J]. CJAP, 2016, 32(4): 305-309.