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CJAP ›› 2016, Vol. 32 ›› Issue (4): 305-309.doi: 10.13459/j.cnki.cjap.2016.04.005

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The effects of leptin on neuron apoptosis in mice with cerebral ischemia/reperfusion injury

SI Yi-ling, ZHANG Jin-ying, DENG Zi-hui, XUE Hui, YAN Guang-tao   

  1. Research Laboratory of Biochemistry, Basic Medical Institute, General Hospital of PLA, Beijing 100853, China
  • Received:2015-11-04 Revised:2016-04-05 Online:2016-07-28 Published:2018-06-20
  • Supported by:
    国家自然科学基金资助项目(30670821)

Abstract: Objective:To study the effect of leptin on neuron apoptosis in mice with cerebral ischemia injury. Methods:Seventy-five male Kuming mice were randomly divided into 3 groups:sham, model and leptin intervention group, respectively. Focal cerebral ischemia/reperfusion injury model in mice was established by middle cerebral artery occlusion. Leptin intervention group was injected with leptin (1μg/g weight, I. P.) at 0 min of ischemic injury. Neuron apoptosis was detected by TUNEL staining. The mRNA expression of apoptosis relative gene bcl-2 and caspase-3 were detected by RT-PCR. The protein expression of bcl-2 and caspase-3 were detected by immunohistochemistry. Results:In model group, most of the neurons in the central area of cerebral ischemia had necrosis obviously, and the amount of neuron apop-tosis was much higher than that in sham group (P<0.01). Compared with sham group, both expression of pro-apoptosis gene caspase-3 and anti-apoptosis gene bcl-2 increased significantly in model group (P<0.01). Compared with model group, the amount of neuron apoptosis and expression level of caspase-3 were decreased significantly (P<0.01), whereas the mRNA and protein expression of bcl-2 were increased sig-nificantly in leptin intervention group (P<0.01). Conclusion:Leptin could reduce neuron apoptosis through down-regulation the expression of caspase-3 and up-regulation the expression of bcl-2. It suggests that leptin could play a neuroprotective role in cerebral ischemia injury.

Key words: leptin, cerebral ischemia, neuron, apoptosis, neuroprotection, mice

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