Effect of aerobic exercise on energy metabolism of skeletal muscle mitochondria in aging rats

doi:10.12047/j.cjap.5631.2018.055

Abstract

Abstract: Objective: To investigate the effect of aerobic exercise on energy metabolism of skeletal muscle mitochondria in aging rats.Methods: Twenty female Wistar rats aging to 12-month old were randomly divided into two groups:aged control group (AC,n=10)and aged exercise group (AE,n=10), and another 10 female Wistar rats aging to 2-month old were set as the young control group(YC,n=10). The rats in YC and AC groups were reared normally, the rats in AE group were given treadmill exercise 6 days a week for 12 weeks (slope 5°, speed 15.2 m/min, 15 min on the 1st day, 30 min on the 2nd day, 45 min on the 3th day and beyond). After 12 weeks, all the rats were decapitated, the gastrocnemius samples were taken, the mitochondria were extracted by differential centrifugation and used for measuring the activities of superoxide dismutase(SOD), glutathione peroxidase(GSH-Px), tricarboxylic acid cycling rate-limiting enzymes citrate synthase(CS), isocitrate dehydrogenase(ICD) and α-ketoglutarate dehydrogenase(α-KGDHC), respiratory chain complexs(RCC)Ⅰ~Ⅳ and the content of malondialdehyde(MDA).Results: ①Compared with YC group, the SOD activity and the MDA content were significantly increased (P<0.05),the activities of CS and α-KGDHC were significantly decreased (P<0.05),the activities of RCCⅠ, RCCⅡ and RCC Ⅳ were significantly decreased (P<0.05), and the activity of RCCⅢ was significantly increased (P<0.05) in skeletal muscle mitochondria of AC group; the activities of SOD and GSH-Px and the MDA content were significantly increased (P<0.01), the activities of CS, ICD and α-KGDHC were significantly increased (P<0.01), the activities of RCCⅠ~Ⅳ were significantly increased (P<0.01) in skeletal muscle mitochondria of AE group.②Compared with AC group, the activities of SOD and GSH-Px were significantly increased (P<0.05), MDA content was significantly decreased (P<0.05), the activities of CS, ICD, α-KGDHC and RCCⅠ~Ⅳwere significantly increased (P<0.01) in AE group.Conclusion: Aerobic exercise can improve the antioxidant capacity of skeletal muscle mitochondria in aging rats, decrease the level of lipid peroxidation, improve the function of tricarboxylic acid cycle and respiratory chain, promote mitochondrial energy metabolism, and slow down the degenerative changes in mitochondria during aging.

Key words: aging, rat, aerobic exercise, mitochondria, free radical metabolism

LI Jie, TAN Wen-bo, WANG Yan. Effect of aerobic exercise on energy metabolism of skeletal muscle mitochondria in aging rats[J]. CJAP, 2018, 34(3): 234-237.

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