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CJAP ›› 2018, Vol. 34 ›› Issue (3): 258-262.doi: 10.12047/j.cjap.5669.2018.061

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Effects of nano-carbon black particles combined with cold exposure on oxidative stress of lung in mice

ZHAO Dan-dan1,2, GUO Zhan2, LI Xi2, ZHANG Yong-qiang2, ZHANG Li2, ZHU Wu-fu1, ZHENG Peng-wu1, YANG Dan-feng2   

  1. 1. School of Pharmacy, Jiangxi Science and Technology Normal University, Nanchang 330013;
    2. Institute of Environmental and Operational Medicine, Institute of Military Medicine, Academy of Military Sciences, Tianjin 30050, China
  • Received:2018-01-08 Revised:2018-02-14 Online:2018-05-28 Published:2018-09-08
  • Supported by:
    国家自然科学基金资助(21707165),BWS16J0101

Abstract: Objective: To study the effects of nano-carbon black particles combined with cold exposure on lung tissue and its oxidative stress in mice.Methods: Seventy-two healthy male C57BL/6 mice were randomly divided into six groups:control group (Ctrl), cold exposure group (C), low dose exposure group (L), low dose exposure combined with cold exposure group (LC), high dose exposure group (H), high dose exposure combined with cold exposure group (HC). The suspensions of nanoscale carbon black particles were intratracheal instilled to mice once time, the exposure doses were 0.45 mg/ml (L) and 4.05 mg/ml (H). The mice in cold group and in nano-carbon black particles exposure combined with cold exposure groups had been intermittently exposed to 4℃ for 20 days (4 h/day). Then the mice were weighed and the lung were drawn. The activities of SOD (superoxide dismutase), GSH-Px (glutathione peroxidase) and the content of MDA (malondialdehyde) in lung were determined. The lung pathological slices were prepared to observe the structural changes of the lung.Results: All cold exposed mice were significantly lighter in body weight than mice in all non-cold-exposed groups(P<0.05),the body weight of the mice in control group and in simple nano-carbon black particles exposure group began to increase significantly after the 14th day of the experiment(P<0.05), the body weight of the mice in the C group, LC group and HC group tended to be stable after the 14th day of the experiment. The results of HE pathological examination showed that the alveolar spaces of mice in L group, LC group, H group and HC group were all deposited with black granules. The alveolar structure was disrupted, the arrangement was in disorder, and a large number of inflammatory cells infiltrated in lung of HC group. Compared with the control group,SOD activity of the other groups was significantly decreased(P<0.05). The GSH-Px activity of the high-dose exposure group and the high-dose exposure combined with cold exposure group was significantly lower than that of the control group (P<0.01). The MDA content in H group, LC group and HC group was significantly higher than that of the control group(P<0.01). The two factor variance analysis showed that as the administrated dose increased,SOD activity and GSH-Px activity were decreased significantly(P<0.05), and as the decrease of temperature, the MDA content of lung tissue was significantly increased(P<0.05). There was no interaction effect between 4℃ intermittent cold exposure and nano-carbon black particles exposure on the activity of SOD, GSH-Px and the content of MDA in lung tissue.Conclusion: Nano-carbon black particles combined with cold exposure might aggravate lung inflammation and increase the level of oxidative stress in mice.

Key words: cold exposure, nano-carbon black particles, mouse, lung, oxidative stress

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