Effects of Huaihuasan and baitouweng formular on rats with acute radiation proctitis and its mechanism

doi:10.12047/j.cjap.6052.2021.016

Abstract

Abstract: Objective: To investigate the effects of huaihuasan and baitouweng formular on acute radiation proctitis (ARP) in rats. Methods: Forty clean grade SD rats were randomly divided into control group, model group, mesalazine group and formula group. Except the control group, all the other three groups received 6 MV-20 GY dose of X-ray irradiation in the pelvic cavity, and the rat model of acute radiation proctitis was established. The rats were given daily gavage intervention with the corresponding drugs after mold formation. According to the adult clinical equivalent dose (body surface area), the control group and the model group were given 10 ml/kg saline daily, the Mesalazine group was treated with Mesalazine solution at the dose of 0.27 g/kg, and the Formular group was given (0.91 g/kg) respectively for 14 days. All rats were killed on the 14th day after administration. To evaluate the general situation of the rats, HE staining was used to observe the pathological changes in the rectal tissues of the rats. The contents of intercellular adhesion molecule-1 (ICAM-1) and vascular adhesion molecule-1 (VCAM-1) were detected by Elisa, and the expression of NF-κb P65 in the tissues were detected by Western blot. Results: Compared with the model group, in the mesalazine group and the formular group, the clinical symptoms and intestinal mucosal healing of rats were improved significantly, the expression level of NF-κB P65 protein in the rectal tissues and the serum contents of ICAM-1 and VCAM-1 were decreased with statistically significant differences (P＜0.05). Conclusion: Huaihuasan and baitouweng formular can reduce NF-κB, ICAM-1 and VCAM-1 to improve symptoms and rectal mucosal injury in ARP rats.

Key words: Huaihuasan, Baitouweng formular, acute radiation proctitis, rat, NF-κB, ICAM-1, VCAM-1

CLC Number:

R73-3

ZHAO Yang, ZHANG Yu-shuang, WANG Yu-xiang, ZHANG Ming, SHI Hui-juan, LI Jing. Effects of Huaihuasan and baitouweng formular on rats with acute radiation proctitis and its mechanism[J]. CJAP, 2021, 37(3): 313-317.

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