氧糖剥夺对人脐静脉内皮细胞通透性的影响

doi:10.12047/j.cjap.5407.2017.027

中国应用生理学杂志 ›› 2017, Vol. 33 ›› Issue (2): 105-108.doi: 10.12047/j.cjap.5407.2017.027

氧糖剥夺对人脐静脉内皮细胞通透性的影响

韩雪¹, 黄欣², 朱玲玲², 范明^1,2

1. 首都医科大学神经生物学系, 北京 100069;
2. 军事医学科学院基础医学研究所认知科学研究室, 北京 100850

收稿日期:2016-03-29 修回日期:2016-11-25 出版日期:2017-03-28 发布日期:2018-06-20
通讯作者: 范明,Tel:010-66931315,E-mail:fanmingchina@126.com;朱玲玲,E-mail:linglingzhu@hotmail.com E-mail:fanmingchina@126.com;linglingzhu@hotmail.com
基金资助:
范明,Tel:010-66931315,E-mail:fanmingchina@126.com;朱玲玲,E-mail:linglingzhu@hotmail.com

Effects of oxygen-glucose deprivation on the permeability of human umbilical vein endothelial cells

HAN Xue¹, HUANG Xin², ZHU Ling-ling², FAN Ming^1,2

1. Department of Neurobiology, Capital Medical University, Beijing 100069;
2. Department of Cognitive Science, Beijing Institute of Basic Medical Science, Beijing 100850, China

Received:2016-03-29 Revised:2016-11-25 Online:2017-03-28 Published:2018-06-20
Contact: 范明,Tel:010-66931315,E-mail:fanmingchina@126.com;朱玲玲,E-mail:linglingzhu@hotmail.com E-mail:fanmingchina@126.com;linglingzhu@hotmail.com
Supported by:
范明,Tel:010-66931315,E-mail:fanmingchina@126.com;朱玲玲,E-mail:linglingzhu@hotmail.com

摘要/Abstract

摘要： 目的：建立体外氧糖剥夺模型模拟脑缺血缺氧损伤，探讨氧糖剥夺对人脐静脉内皮细胞（HUVECS）屏障功能的影响。方法：细胞培养至完全融合后换成无糖培养基置于低氧手套箱（0.3% O₂）分别处理0.5 h、1 h、2 h和4 h后，利用CCk-8法检测细胞存活，利用跨内皮细胞电阻（trans-endothelial electrical resistant，TEER）方法检测HU-VECs细胞通透性的变化，以及Western blot检测紧密连接相关蛋白的表达。结果：氧糖剥夺处理0.5 h、1 h、2 h和4h后，HUVECs细胞存活率逐渐下降，TEER值逐渐降低，紧密连接蛋白Occludin、VE-cadherin的表达明显降低。结论：氧糖剥夺破坏内皮细胞间的紧密连接功能，增加HUVECs细胞的通透性，导致细胞的存活率明显降低。

关键词: 氧糖剥夺, 紧密连接, 通透性, HUVEC

Abstract: Objective: To investigate the effects of oxygen-glucose deprivation (OGD) on the functions of blood-brain barrier in vitro, we established an OGD model to mimic cerebral ischemic injury in human umbilical vein endothelial cells (HUVECs). Methods: Cells were cul-tured in DMEM without glucose under hypoxic conditions (0.3% O₂) for different time point. The survival rate of HUVECs was detected by CCK-8 assay. The permeability of HUVECs was measured by epithelial volt-ohm meter. The expression of tight junction protein was detected by Western blot analysis. Results: In OGD group, the survival rate of HUVECs was obviously decreased. The permeability of HUVECs also decreased in OGD-treated cells. And the expression of tight junction protein was reduced after OGD treatment. Conclusion: Our data indicate that OGD treatment disrupt the barrier function of tight junctions and increase permeability of HUVECs and finally accelerated cell death.

Key words: oxygen-glucose deprivation, tight junction, permeability, HUVEC

韩雪, 黄欣, 朱玲玲, 范明. 氧糖剥夺对人脐静脉内皮细胞通透性的影响[J]. 中国应用生理学杂志, 2017, 33(2): 105-108.

HAN Xue, HUANG Xin, ZHU Ling-ling, FAN Ming. Effects of oxygen-glucose deprivation on the permeability of human umbilical vein endothelial cells[J]. CJAP, 2017, 33(2): 105-108.

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氧糖剥夺对人脐静脉内皮细胞通透性的影响

Effects of oxygen-glucose deprivation on the permeability of human umbilical vein endothelial cells

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